Vancomycin-dendrimer based multivalent magnetic separation nanoplatforms combined with multiplex quantitative PCR assay for detecting pathogenic bacteria in human blood

Feng, Xiaoyan; Meng, Xiangyu; Xiao, Fangbin; Aguilar, Zoraida P.; Xu, Hengyi

doi:10.1016/j.talanta.2020.121953

Cited by 21 publications

(17 citation statements)

References 35 publications

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“…The results suggest that the use of van-PDA-MNP eliminated the non-speci c adsorption of blood cells and the formation of aggregates in the blood, which resulted in successful removal of the PCR inhibitory compounds and improved LOD. As previously reported [23,32], van and lectin conjugated with MNPs were used to magnetically separate bacteria from blood. As the conventionally used MNPs interact with blood cells and form aggregates, they have included an extra step for treating whole blood to remove the blood cells through natural sedimentation [23] or centrifugation after they have lysed red blood cells [32].…”

Section: Preconcentration Of Different Species Of Bacteria With Van-p...mentioning

confidence: 99%

“…As previously reported [23,32], van and lectin conjugated with MNPs were used to magnetically separate bacteria from blood. As the conventionally used MNPs interact with blood cells and form aggregates, they have included an extra step for treating whole blood to remove the blood cells through natural sedimentation [23] or centrifugation after they have lysed red blood cells [32]. Because of the ability of the PDA coating to prevent non-speci c adsorption of blood cells and aggregation, van-PDA-MNPs can be used to capture and preconcentrate target bacteria from whole blood without the need for additional pretreatment steps.…”

Section: Preconcentration Of Different Species Of Bacteria With Van-p...mentioning

confidence: 99%

“…To isolate a broad range of bacterial species, antibiotics can be conjugated to MNPs instead of speciesspeci c antibodies [23,24]. Among antibiotics, van has been conjugated to MNPs to isolate Grampositive bacteria [24].…”

Section: Introductionmentioning

confidence: 99%

“…Van is a bacteriostatic antibiotic that inhibits bacterial cell wall synthesis by binding to the D-alanyl-D-alanyl terminus of the peptidoglycan layer of Gram-positive bacteria through hydrogen bonding [25,26]. Therefore, it has great potential for use in the separation of Gram-positive bacteria [23,24]. For example, van-MNPs were used to separate Gram-positive bacteria such as Staphylococcus aureus from whole blood and improved the sensitivity of quantitative PCR (qPCR) to about 5 colony forming units (CFU) per 1 mL of blood.…”

Section: Introductionmentioning

confidence: 99%

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Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood

Abafogi

Wu²,

Lee

et al. 2022

Preprint

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Sepsis is caused mainly by infection in the blood with a broad range of bacterial species. It can be diagnosed by molecular diagnostics once compounds in the blood that interfere with molecular diagnostics are removed. However, this removal relies on ultracentrifugation. Immunomagnetic separation (IMS), which typically uses antibody-conjugated silica-coated magnetic nanoparticles (Ab-SiO2-MNPs), has been widely applied to isolate specific pathogens in various types of samples, such as food and environmental samples. However, its direct use in blood samples containing bacteria is limited due to the aggregation of SiO2-MNPs in the blood and inability to isolate multiple species of bacteria causing sepsis. Results: In this study, we report the synthesis of vancomycin-conjugated polydopamine-coated (van-PDA-MNPs) enabling preconcentration of multiple bacterial species from blood without aggregation. The presence of PDA and van on MNPs was verified using transmission electron microscopy, X-ray photoelectron spectroscopy and energy disruptive spectroscopy. Unlike van-SiO2-MNPs, van-PDA-MNPs did not aggregate in the blood. Van-PDA-MNPs were able to preconcentrate several species of Gram-positive bacteria in the blood, lowering the limit of detection (LOD) to 10 colony forming units/mL by polymerase chain reaction (PCR) and quantitative PCR. This is 10 times more sensitive than the LOD obtained by PCR and qPCR with the use of van-SiO2-MNPs. Conclusion: These results suggest that PDA-MNPs do not aggregate in blood and can be conjugated with receptors, thereby improving the sensitivity of molecular diagnostics for bacteria in blood samples.

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Section: Preconcentration Of Different Species Of Bacteria With Van-p...mentioning

confidence: 99%

Section: Preconcentration Of Different Species Of Bacteria With Van-p...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood

Abafogi

Wu²,

Lee

et al. 2022

Preprint

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show abstract

“…Low concentrations of bacteria in the environment can cause people to suffer from possible diseases, but these concentrations are often far beyond the detection range of instruments. Previous microbiology methods, including bacterial culture [ 9 ] or multiplex polymerase chain reaction (PCR) assay [ 10 , 11 ], immunology-based methods [ 12 , 13 ], have the following problems: long detection time, vulnerability to microbial contamination, limited laboratories of professional inspection institutions, and the inability to enrich and detect bacteria on-site in time [ 14 ]. Therefore, efficient enrichment of bacteria is a necessary and urgent matter, and the commonly used nanocomposite for electrostatic adsorption of bacteria are polyethersulfone nanofibrous membranes [ 15 ], flexible graphene oxide (GO) microsheets [ 16 ], and so on, but the production conditions of these materials are harsh, and the production process is complex.…”

Section: Introductionmentioning

confidence: 99%

Fe3O4@PDA@PEI Core-Shell Microspheres as a Novel Magnetic Sorbent for the Rapid and Broad-Spectrum Separation of Bacteria in Liquid Phase

Zhang¹,

Du²,

Wu³

et al. 2022

Materials

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Bacterial infection is a significant cause of morbidity and mortality to humans worldwide. Thus, a method for nonspecific, sensitive, and rapid enrichment of such bacteria is essential for bacteria detection and treatment. This study demonstrates a self-made core-shell Fe3O4@Polydopamine@Polyethyleneimine magnetic beads (Fe3O4@PDA@PEI MBs) with a high density positive charge-based magnetic separation scheme for the broad-spectrum rapid enrichment of microorganisms in the liquid phase. MBs with a high-density positive charge have a strong electrostatic attraction to most microorganisms in nature. Our scheme is as follows: (1) wrapping dopamine (DA) on the iron oxide through self-polymerization and wrapping PEI on the outermost shell layer in a mode of crosslinking with the PDA; (2) subsequently, the Fe3O4@PDA@PEI MBs were used to concentrate microorganisms from the sample solution; (3) performing magnetic separation and calculating the adsorption efficiency. The as-prepared Fe3O4@PDA@PEI MBs composite was carefully characterized by zeta potential analysis, Value stream-mapping (VSM), transmission electron microscopy (TEM), and Fourier transforms infrared spectrometry (FT-IR). In this study, both gram-positive and gram-negative bacteria could be captured in three minutes through electrostatic interaction. Furthermore, the adsorption efficiency on gram-negative (>98%) is higher than that on gram-positive (>95%), allowing for a simple, rapid assay to enrich organisms in resource-limited settings.

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Establishment of a Fast Diagnostic Method for Sepsis Pathogens Based on M1 Bead Enrichment

Zheng

Chen

et al. 2023

Curr Microbiol

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Vancomycin-dendrimer based multivalent magnetic separation nanoplatforms combined with multiplex quantitative PCR assay for detecting pathogenic bacteria in human blood

Cited by 21 publications

References 35 publications

Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood

Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood

Fe3O4@PDA@PEI Core-Shell Microspheres as a Novel Magnetic Sorbent for the Rapid and Broad-Spectrum Separation of Bacteria in Liquid Phase

Establishment of a Fast Diagnostic Method for Sepsis Pathogens Based on M1 Bead Enrichment

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