Vancomycin and creatinine determination in dried blood spots: Analytical validation and clinical assessment

Zavascki, Alexandre Prehn; Matos, Douglas Marinho de; Silveira, Francine; Peralta, Talitha; Landgraf, Natalia Gonçalves; Wink, Priscila Lamb; Silva, Anne Caroline Cezimbra da; Andriguetti, Nadine Bordin; Lisboa, Letícia Loss; Antunes, Marina Venzon; Linden, Rafael

doi:10.1016/j.jchromb.2019.121897

Cited by 19 publications

(9 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some difficulties associated with DBS analysis include the variation in the physio-pathological state of the patients, which affects the haematocrit [15] . Predicting plasma concentrations from DBS samples is complicated by the variation in haematocrit that can lead to varying spot sizes, which could cause bias [41] .…”

Section: Resultsmentioning

confidence: 99%

“…Traditional sampling methods are often resource-intensive at both the collection and storage chain stages [11] , [12] . Quantifying analytes in dried blood spots (DBS) has several advantages when compared to traditional sampling methods, being particularly useful in settings of resource scarcity [13] , [14] , [15] . DBS sample preparation can be performed without the use of specialized laboratory apparatus, such as centrifuges, and, therefore, untrained staff, or even the patients themselves, can prepare the samples [11] , [14] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Validation of a quantitative multiplex LC-MS/MS assay of carvedilol, enalaprilat, and perindoprilat in dried blood spots from heart failure patients and its cross validation with a plasma assay

Joubert

Merwe

et al. 2023

Journal of Mass Spectrometry and Advances in the Clinical Lab

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Validation of a quantitative multiplex LC-MS/MS assay of carvedilol, enalaprilat, and perindoprilat in dried blood spots from heart failure patients and its cross validation with a plasma assay

Joubert

Merwe

et al. 2023

Journal of Mass Spectrometry and Advances in the Clinical Lab

View full text Add to dashboard Cite

“…21 The concentrations of vancomycin in capillary DBS and plasma samples collected from 29 patients receiving vancomycin therapy were evaluated in a previous study. 26 The study demonstrated that the concentrations of vancomycin in the plasma could not be accurately predicted by the DBS strategy, probably because of the variability in the partitioning between the plasma and the red blood cells or the interactions with the substrate of the DBS paper. The comparisons between the DBS and plasma concentrations in these studies were useful in establishing the DBS-plasma correlation, and the DBS method was shown to be promising when corrected for HCT.…”

Section: Clinical Applicationmentioning

confidence: 93%

Microsampling Assays for Pharmacokinetic Analysis and Therapeutic Drug Monitoring of Antimicrobial Drugs in Children: A Critical Review

Moorthy

Vedar

Downes

et al. 2021

Therapeutic Drug Monitoring

View full text Add to dashboard Cite

Background: With the increasing prevalence of multidrug resistant organisms, therapeutic drug monitoring (TDM) has become a common tool for assuring the safety and efficacy of antimicrobial drugs at higher doses. Microsampling techniques, including dried blood spotting (DBS) and volumetric absorptive microsampling (VAMS), are attractive tools for TDM and pediatric clinical research. For microsampling techniques to be a useful tool for TDM, it is necessary to establish the blood-plasma correlation and the therapeutic window of antimicrobial drugs in the blood.Methods: DBS involves the collection of small volumes of blood (30-50 mL per spot) on a filter paper, whereas VAMS allows the accurate and precise collection of a fixed volume of blood (10-30 mL) with microsampling devices. One of the major advantages of VAMS is that it reduces or eliminates the volumetric blood hematocrit (HCT) bias associated with DBS. Liquid chromatography with tandem mass spectrometry is a powerful tool for the accurate quantification of antimicrobial drugs from small volumes of blood specimens.Results: This review summarizes the recent liquid chromatography with tandem mass spectrometry assays that have used DBS and VAMS approaches for quantifying antimicrobial drugs. Sample collection, extraction, validation outcomes, including the interassay and intra-assay accuracy and precision, recovery, stability, and matrix effect, as well as the clinical application of these assays and their potential as tools of TDM are discussed herein. Conclusions:Microsampling techniques, such as VAMS, provide an alternative approach to traditional plasma sample collection for TDM.

show abstract

“…Barco et al published an analytical method for the simultaneous determination of 14 antibiotics (including VAN, MEM, and LZN) in plasma ( 11 ) but did not address microsampling. Several methods utilize DBS or CMS samples to separately determine the three antibiotics described in the literature ( 12 – 15 ). From a clinical standpoint, small-volume sampling and simultaneous determination of the three antibiotics could be useful in pediatric ICUs.…”

Section: Introductionmentioning

confidence: 99%

Applicability of vancomycin, meropenem, and linezolid in capillary microsamples vs. dried blood spots: A pilot study for microsampling in critically ill children

Xiaoyong

Wang

et al. 2023

Front. Pediatr.

View full text Add to dashboard Cite

IntroductionTherapeutic drug monitoring (TDM) has been shown to be clinically beneficial for critically ill patients. However, this is a burden for neonates or children with small circulating blood volumes. Here, we aimed to develop and validate a microsampling TDM platform (including dried blood spots (DBS) and capillary microsamples (CMS)) for the simultaneous quantification of vancomycin, meropenem, and linezolid.MethodsPaired DBS and CMS samples were obtained from an intensive care unit (ICU) to evaluate its clinical application. Estimated plasma concentrations (EPC) were calculated from DBS concentrations. Agreement between methods was evaluated using Deming regression and Bland-Altman difference plots.ResultsThe microsampling methods validation showed excellent reliability and compatibility with the analysis of the sample matrix and hematocrit range of the studied population. The DBS and CMS accuracy and precision results were within accepted ranges and samples were stable at room temperature for at least 2 days and 8 h, respectively. Hematocrit had no impact on CMS, but sightly impacted DBS measurements. The CMS and DBS antibiotic concentrations correlated well (r > 0.98). The drug concentration ratio in DBS samples to that in CMS was 1.39 for vancomycin, 1.34 for meropenem, and 0.94 for linezolid. The EPC calculated from the DBS using individual hematocrit ranges presented comparable absolute values for vancomycin (slope: 1.06) and meropenem (slope: 1.04), with a mean of 98% and 99% of the measured CMS concentrations, respectively.DiscussionThis study provides a microsampling TDM platform validated for clinical use for a rapid quantification of three antibiotics and is suitable for real-time TDM-guided personalization of antimicrobial treatment in critically ill children.

show abstract

Vancomycin and creatinine determination in dried blood spots: Analytical validation and clinical assessment

Cited by 19 publications

References 23 publications

Validation of a quantitative multiplex LC-MS/MS assay of carvedilol, enalaprilat, and perindoprilat in dried blood spots from heart failure patients and its cross validation with a plasma assay

Validation of a quantitative multiplex LC-MS/MS assay of carvedilol, enalaprilat, and perindoprilat in dried blood spots from heart failure patients and its cross validation with a plasma assay

Microsampling Assays for Pharmacokinetic Analysis and Therapeutic Drug Monitoring of Antimicrobial Drugs in Children: A Critical Review

Applicability of vancomycin, meropenem, and linezolid in capillary microsamples vs. dried blood spots: A pilot study for microsampling in critically ill children

Contact Info

Product

Resources

About