Value of transferring embryos that show no evidence of fertilization at the time of fertilization assessment

Li, Ming; Lin, Shengli; Yuan, Chen; Zhu, Junjie; Liu, Ping; Qiao, Jie

doi:10.1016/j.fertnstert.2015.05.016

Cited by 24 publications

(41 citation statements)

References 18 publications

(32 reference statements)

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“…In the present study, when 0PN was obtained by ICSI, the clinical outcome was nearly the same as that for 2PN, but when 0PN was obtained by IVF, the clinical outcome was worse than that for 2PN. Li et al, previously compared the implantation rates of frozen‐thawed 0PN‐derived blastocysts obtained by different fertilization methods; they found a difference, albeit not significant, between IVF and ICSI, at 35.59% (21 out of 59) and 45.45% (15 out of 33), respectively. The results of the present study were similar.…”

Section: Discussionsupporting

confidence: 83%

“…Noyes et al, reported that the diploidy rate in 0PN‐derived embryos on day 3 was only 3%, and the blastocyst stage was not reached even when 0PN culture was extended. All embryos included in the present study were frozen when they reached the blastocyst stage, and were therefore different from the embryos studied by Noyes et al, Previous reports have indicated that blastocyst culture is effective for selecting embryos with normal chromosomes among 1PN‐ and 0PN‐derived embryos, and it is probable that in the present study, 0PN‐derived embryos with aberrant chromosomes were eliminated. Li et al, found that the implantation rate was lower for 0PN‐derived embryos than for 2PN‐derived embryos when frozen‐thawed cleavage‐phase transfer was performed.…”

Section: Discussionmentioning

confidence: 58%

“…Li et al, found that the implantation rate was lower for 0PN‐derived embryos than for 2PN‐derived embryos when frozen‐thawed cleavage‐phase transfer was performed. However, the implantation rates were nearly the same with blastocyst transfer. Liao et al, reported a significantly higher diploidy rate for 1PN‐derived blastocysts (74.6%) than for 1PN with halted cleavage (31.6%).…”

Section: Discussionmentioning

confidence: 94%

“…Chromosomal analysis has shown that some 1PN‐ and 0PN‐derived embryos are diploid and have normal chromosomal structure. Furthermore, births of healthy infants resulting from the transfer of such blastocysts have been reported.…”

Section: Introductionmentioning

confidence: 99%

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Clinical outcomes of transfer of frozen and thawed single blastocysts derived from nonpronuclear and monopronuclear zygotes

Hondo¹,

Arichi²,

Muramatsu³

et al. 2019

Reprod Medicine & Biology

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Purpose In assisted reproductive technology, normal zygotes are bipronuclear (2PN) during fertilization confirmation; however, sometimes, nonpronuclear zygotes (0PN) and monopronuclear zygotes (1PN) are found during routine observations. Methods To elucidate the clinical usefulness of in vitro‐fertilized embryos, we investigated the rates of clinical pregnancy, live birth, miscarriage, and congenital abnormality after transfer of frozen‐thawed 1PN‐ and 0PN‐derived single blastocysts at Denentoshi Ladies Clinic, Kanagawa, Japan. Results The rates of pregnancy and live birth for 1PN‐derived blastocysts obtained by conventional in vitro fertilization were 37.5% and 27.1%, respectively, which was not significantly different from those for 2PN‐derived blastocysts; however, the rates for 0PN‐derived blastocysts were significantly lower. The pregnancy and live birth rates for 0PN‐derived embryos obtained by intracytoplasmic sperm injection (ICSI) were 45.7% and 34.8%, respectively, which was not significantly different from those for 2PN‐derived blastocysts; however, the rates for 1PN‐derived blastocysts were significantly lower (4.0% for both) than those for 2PN‐ and 0PN‐derived blastocysts. No congenital abnormalities were found in infants resulting from transfer of 0PN‐ or 1PN‐derived blastocysts. Conclusions Both 1PN‐ and 0PN‐derived blastocysts can be used for embryo transfer; however, care should be taken in making decisions about 1PN‐derived blastocysts, especially if they are obtained by ICSI.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Clinical outcomes of transfer of frozen and thawed single blastocysts derived from nonpronuclear and monopronuclear zygotes

Hondo¹,

Arichi²,

Muramatsu³

et al. 2019

Reprod Medicine & Biology

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“…However, non-and/or mono-pronuclear embryos could be transferred for patients lacking bi-pronuclear embryos, especially those with poor ovarian response. The value of non-bi-pronuclear embryo transfer has been discussed in a recent article by Li et al [2015]. Until now, there are no standard criteria for selecting embryos with the highest developmental potential in patients without normal bi-pronuclear embryos.…”

Section: Introductionmentioning

confidence: 99%

Good quality blastocyst from non-/mono-pronuclear zygote may be used for transfer during IVF

Yin

Hao

Zhang

et al. 2016

Systems Biology in Reproductive Medicine

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Although healthy infants have developed from non-and mono-pronuclear zygotes, the transfer of embryos from non-and mono-pronuclear zygotes is not recommended because there are no proper selection criteria. In the present study, we discuss how to select non-and mono-pronuclear embryos with the highest developmental potential at 19-20 hours post-insemination. We found that the percentage of blastocysts with normal chromosome constitution in non-pronuclear zygotes was slightly higher than in mono-pronuclear zygotes. Non-and mono-pronuclear embryos that were at the 4-cell stage on D2 and/or at the 6-to 8-cell stage on D3 had higher incidence rates of blastocysts with normal chromosome constitutions. We also found higher incidences of blastocysts with normal chromosome constitution on D6 than on D5. The results suggest that if high quality non-and mono-pronuclear zygotes develop to the 4-cell stage on D2 and the 6-to 8-cell stages on D3, along with high quality D6 blastocysts, the incidence of blastocysts with normal chromosome constitution is higher. ARTICLE HISTORY

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Clinical outcomes of frozen–thawed blastocysts from zygotes with no or one pronucleus for in vitro fertilization and intracytoplasmic sperm injection cycles

Jin

Xue

Liu

et al. 2023

Arch Gynecol Obstet

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Purpose To investigate the clinical outcomes of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles using frozen–thawed blastocyst transfers derived from zygotes with no (0PN) or one pronucleus (1PN). Methods This retrospective study included 7084 0PN, 2238 1PN, and 72,266 two pronuclear (2PN) embryos cultured to the blastocyst stage from 19,631 IVF and 12,377 ICSI cycles between March 2018 and December 2021. Developmental potential and clinical outcomes of 0PN, 1PN, and 2PN embryos were analyzed. A total of 290 0PN-, 92 1PN-, and 1906 2PN-derived single frozen–thawed blastocyst transfers were performed. Chromosome euploid rates of 0PN-, 1PN-, and 2PN-derived blastocysts were analyzed by next-generation sequencing. Euploid 0PN- and 1PN-derived blastocysts underwent subsequent Infinium Asian Screening Array gene chip analysis to detect ploidy alterations. Results Available blastocyst rates of 0PN and 1PN embryos were significantly lower than those of 2PN embryos in both IVF and ICSI cycles. Single 0PN and 1PN blastocysts transferred in frozen–thawed cycles resulted in a similar clinical pregnancy rate, miscarriage rate, live birth rate, and neonatal outcome to 2PN blastocysts in IVF and ICSI cycles. Genetic analysis showed that euploid rates of 0PN- and 1PN-derived blastocysts used for ICSI cycles were similar to that of 2PN-derived blastocysts. Conclusion Our study indicated that 0PN- and 1PN-derived blastocysts resulted in similar clinical outcomes to 2PN-derived blastocysts. The 0PN- and 1PN-derived blastocysts from ICSI cycles can be transferred as well as those from IVF cycles when the number of 2PN-derived blastocysts is insufficient.

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Value of transferring embryos that show no evidence of fertilization at the time of fertilization assessment

Cited by 24 publications

References 18 publications

Clinical outcomes of transfer of frozen and thawed single blastocysts derived from nonpronuclear and monopronuclear zygotes

Clinical outcomes of transfer of frozen and thawed single blastocysts derived from nonpronuclear and monopronuclear zygotes

Good quality blastocyst from non-/mono-pronuclear zygote may be used for transfer during IVF

Clinical outcomes of frozen–thawed blastocysts from zygotes with no or one pronucleus for in vitro fertilization and intracytoplasmic sperm injection cycles

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