Value of the mixed lymphocyte reaction in dogs as a genetic assay

Bijnen, A. B.; Dekkers-Bijma, Amelie M.; Vriesendorp, Huibert M.; Westbroek, D. L.

doi:10.1007/bf01561440

Cited by 9 publications

(2 citation statements)

References 12 publications

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“…For both groups of experimental animals, only dogs were selected that were unrelated and DLA-incompatible. MLC tests for monitoring of immune reactivity were performed in a similar manner as for DLA class TI typing [12]. The results were expressed as stimulation index (SI).…”

Section: Dla Matching and Mlc Monitoringmentioning

confidence: 99%

Induction of specific inhibition of alloreactivity in beagle dogs by intrathymic injection of donor splenocytes

Marquet

Bruin

IJzermans

1996

Transplant International

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The aim of this study was to investigate whether intrathymic injection (ITI) of donor splenocytes in dogs might lead to specific immunomodulation as assessed by mixed lymphocyte culture (MLC) tests. Two groups of five beagles each were used. Group 1 containedanimals that were 2 years old, group 2 consisted of animals that were 6 months old. One animal was splenectomized per experimental group and 1 x lo9 splenocytes were injected into the thymic lobes or thymic remnant of the four remaining dogs. On the day of IT1 the dogs were treated subcutaneously with a single dose of 2 ml/kg antilymphocyte serum (ALS). In group 1 the thymus of all dogs was found to be atrophic. IT1 in this group did not result in a decreased immunoreactivity but rather in an enhanced immune response. In group 2 the thymus was still clearly present and IT1 was easy to perform. IT1 induced a significant reduction of specific MLC reactivity at 1 week after treatment. The effect was transient and not significantly further diminished at week 2. These results indicate that IT1 is a technically feasible procedure in a preclinical animal model. It may induce temporary sensitization as well as immunosuppression, possibly depending on the age of the recipient.

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Section: Dla Matching and Mlc Monitoringmentioning

confidence: 99%

Induction of specific inhibition of alloreactivity in beagle dogs by intrathymic injection of donor splenocytes

Marquet

Bruin

IJzermans

1996

Transplant International

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“…Mongrels and beagles were typed for the conventional DLA-A, B, and C locus antigens using a panel of 60 to 90 allo-antisera in one-and two-stage microlymphocytoxicity assays [8]. Donor-host selection was further based on the outcome of mixed lymphocyte culture (MLC) tests, as described earlier [9]. Kidney transplantation was performed only in mongrel-beagle combinations that were MLC positive.…”

Section: Animalsmentioning

confidence: 99%

Abrogation of the beneficial blood transfusion effect in dogs by splenectomy

et al. 1984

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The role of the spleen in the manifestation of the transfusion phenomenon was studied in beagle dogs. Beagles were transfused 3 times with 100 ml blood from different mismatched mongrel dogs, at 4, 3, and 2 weeks before mongrel kidney transplantation or splenectomy. Seven transfused dogs were splenectomized 1 week before transplantation, 9 transfused animals were not splenectomized, and a group of 9 kidney allografted controls was neither transfused nor splenectomized. All recipients were given postoperative immunosuppression, which consisted of azathioprine and prednisolone.As with earlier demonstrations it was found that 3 pretransplant blood transfusions in the nonsplenectomized group led to a significant prolongation of kidney graft survival. All transfused animals survived for more than 3 weeks, a period in which more than 50% of the kidneys in the control group were rejected. Four out of 9 transfused dogs became long-term survivors. In the transfused-splenectomized group, 70% of the recipients rejected their kidney acutely within 3 weeks, and only one animal became a long-term survivor. The absence of a transfusion effect in most of the splenectomized dogs suggests that, under the experimental conditions employed, the spleen plays a crucial role for the induction and manifestation of the transfusion phenomenon.

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A comparative study on changes in hemostasis in orthotopic and auxiliary liver transplantation in pigs

Porte¹,

Blankensteijn

Knot³

et al. 1991

Transplant Int

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We compared blood loss and hemostasis in pigs which had undergone either orthotopic liver transplantation (OLT) (group A, n = 12) or auxiliary heterotopic partial liver transplantation (APLT) (group B, n = 11). Blood samples were taken at regular intervals during and after the operations. In both groups, nine animals survived longer than 24 h and data from these animals were used for analysis. Median (range) intraoperative blood loss was 825 ml (250-1500 ml) in OLT and 425 ml (300-750) in APLT (P less than 0.01). Routine clotting times, as the activated partial thromboplastin time, prothrombin time and thrombin time, showed no major intraoperative changes in either group. Fibrinogen levels decreased in both groups, but no significant difference was found between the two groups. The only significant difference between group A and B was a more sustained increase in fibrinolytic activity after graft recirculation in group A. Postoperatively, restoration of fibrinogen, antithrombin-III and alpha 2-antiplasmin levels was slightly faster in group B, resulting in significantly higher levels during the first day. We conclude that, in this animal model, APLT is associated with significantly lower blood loss and less severe fibrinolytic activity, than OLT. This difference might result from the lack of an anhepatic period and the reduced surgical trauma in auxiliary heterotopic liver transplantation.

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Value of the mixed lymphocyte reaction in dogs as a genetic assay

Cited by 9 publications

References 12 publications

Induction of specific inhibition of alloreactivity in beagle dogs by intrathymic injection of donor splenocytes

Induction of specific inhibition of alloreactivity in beagle dogs by intrathymic injection of donor splenocytes

Abrogation of the beneficial blood transfusion effect in dogs by splenectomy

A comparative study on changes in hemostasis in orthotopic and auxiliary liver transplantation in pigs

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