Value of the Innovated Technique Agarose Cell Block in Improving the Sensitivity of Urine Cytology in Cases of Bladder Carcinoma

Mansy, Soheir S.; Abbas, Mahmoud A; Yehia, Hoda; Abdelrazik, Samia M; Ghanem, Lobna Y; Amin, Tarek

doi:10.1080/01913120600854269

Cited by 12 publications

(11 citation statements)

References 16 publications

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“…The cooled, solidified agar lump is easily falling out of the tube when turned upside down, if not formalin or saline can be injected under it with a small needle. The lump then floats to the top of the tube [22, 23]. Heat artifacts from molten agarose heating processing affect morphology [24].…”

Section: The Agarose Methodsmentioning

confidence: 99%

Cell Block in Cytological Diagnostics: Review of Preparatory Techniques

Krogerus

Kholová

2018

Acta Cytologica

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Objective: The cell block (CB) technique refers to the processing of sediments, blood clots, or grossly visible tissue fragments from cytological specimens into paraffin blocks that can be cut and stained by the same methods used for histopathology. The technique brings additional tissue architectural information. CB can be used for ancillary techniques such as immunocytochemistry and molecular techniques. Study Design: We reviewed the literature on the various preparatory techniques of CBs. Results: There is a wide range of preparatory techniques for CBs and no golden standard for CBs exists: tens of methods are used in various institutions. The majority of the methods are modified in house techniques with a few commercially available kits. The techniques most commonly used are the plasma/thrombin method, the agar method, and commercially available Histogel- and Cellient CB-methods. Dissatisfaction with the cellular yield of the CBs is common. Conclusions: In the CBs, the cytological material is preserved for future use, which is a tremendous advantage in the era of targeted therapy and biobanking. The CB is thus central to the future of cytology: more can be done with less material and with less invasiveness to the patient.

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Section: The Agarose Methodsmentioning

confidence: 99%

Cell Block in Cytological Diagnostics: Review of Preparatory Techniques

Krogerus

Kholová

2018

Acta Cytologica

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“…CB provides more pathological information, when combined with histological examination such as hematoxylin and eosin (H&E) staining and immunostaining of serial sections compared with each method alone. Several studies have reported that CB improves diagnostic efficacy, and the implementation of immunocytochemistry is remarkably useful for the discovery of non-morphological markers [6], [7]. However, very few studies have evaluated and summarized the performance of these 3 methods in the examination of EUS-FNA samples.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Efficacy of Cell Block Immunohistochemistry, Smear Cytology, and Liquid-Based Cytology in Endoscopic Ultrasound-Guided Fine-Needle Aspiration of Pancreatic Lesions: A Single-Institution Experience

Qin

Zhou

et al. 2014

PLoS ONE

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BackgroundThe diagnostic efficiency of endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) cytology varies widely depending on the treatment method of the specimens. The present study aimed to evaluate the diagnostic efficacy of cell block (CB) immunohistochemistry, smear cytology (SC), and liquid-based cytology (LBC) in patients with pancreatic lesions without consulting an on-site cytopathologist.MethodsThis study prospectively enrolled 72 patients with pancreatic lesions. The EUS-FNA specimens were examined by SC, LBC, and CB immunohistochemistry. The diagnostic efficacy of the 3 methods was then compared. Patients’ final diagnosis was confirmed by surgical resection specimens, diagnostic imaging, and clinical follow-up.ResultsOur results included 60 malignant and 12 benign pancreatic lesions. The diagnostic sensitivity (90%), negative predictive value (66.7%), and accuracy (91.7%) of CB immunohistochemistry were significantly higher than those of SC (70.0%, 30.0%, and 75.0%, respectively) and LBC (73.3%, 31.6%, and 77.8%, respectively) (all P<0.05). The combination of CB and SC, or CB and LBC, did not significantly increase the efficacy compared to CB immunohistochemistry alone.ConclusionOur findings suggest that in the absence of an on-site cytopathologist, CB immunohistochemistry on EUS-FNA specimens offers a higher diagnostic efficacy in patients with pancreatic lesions than does SC and LBC.

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“…Our criteria for calling cell lines as SCLC were as follows: (I) pathological diagnosis of SCLC of the original diagnostic material; (II) cell culture with the growth properties characteristic of SCLC; (III) morphological features of classic or variant SCLC by examination of formalin fixed, paraffin embedded agarose cell blocks (20) or cell line derived xenografts (CCXs); and (IV) loss of Rb protein expression by Western blotting (21), or inactivating mutations of the RB1 gene by sequencing. As rare SCLC tumors lack inactivation of RB1 (17), in one case we identified a cell line with intact Rb protein expression as SCLC.…”

Section: Identification Of Sclc Cell Linesmentioning

confidence: 99%

Small cell lung cancer tumors and preclinical models display heterogeneity of neuroendocrine phenotypes

Zhang

Girard²,

Zhang

et al. 2018

Transl. Lung Cancer Res.

198

250

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Background: Small cell lung cancer (SCLC) is a deadly, high grade neuroendocrine (NE) tumor without recognized morphologic heterogeneity. However, over 30 years ago we described a SCLC subtype with "variant" morphology which did not express some NE markers and exhibited more aggressive growth. Methods:To quantitate NE properties of SCLCs, we developed a 50-gene expression-based NE score that could be applied to human SCLC tumors and cell lines, and genetically engineered mouse (GEM) models. We identified high and low NE subtypes of SCLC in all of our sample types, and characterized their properties. Results:We found that 16% of human SCLC tumors and 10% of SCLC cell lines were of the low NE

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Value of the Innovated Technique Agarose Cell Block in Improving the Sensitivity of Urine Cytology in Cases of Bladder Carcinoma

Cited by 12 publications

References 16 publications

Cell Block in Cytological Diagnostics: Review of Preparatory Techniques

Cell Block in Cytological Diagnostics: Review of Preparatory Techniques

Diagnostic Efficacy of Cell Block Immunohistochemistry, Smear Cytology, and Liquid-Based Cytology in Endoscopic Ultrasound-Guided Fine-Needle Aspiration of Pancreatic Lesions: A Single-Institution Experience

Small cell lung cancer tumors and preclinical models display heterogeneity of neuroendocrine phenotypes

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