Value of Molecular Epidemiologic Analysis in a Nosocomial Methicillin-Resistant Staphylococcus aureus Outbreak

Fang, Ferric C.; McClelland, Michael; Guiney, Donald G.; Jackson, Marguerite M.; Hartstein, Alan I.; Morthland, Virginia H.; Davis, Charles E.; McPherson, Diana C.; Welsh, John

doi:10.1001/jama.1993.03510110063033

Cited by 35 publications

(14 citation statements)

References 38 publications

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“…Whenever possible, isolates should be analyzed by a genetic typing system, such as pulsed-field gel electrophoresis of genomic DNA with or without hybridization studies using gene probes, or electrophoresis of PCR amplified sequences [55][56][57][58][59][60][61][62]. In the following section we describe only a few of the most frequently used older and newer typing methods for methicillin-resistant staphylococci.…”

Section: Epidemiological Utility Of Typing Systemsmentioning

confidence: 99%

Epidemiology of Methicillin-Resistant <i>Staphylococcus aureus</i>

Flückiger

Widmer

1999

Chemotherapy

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Section: Epidemiological Utility Of Typing Systemsmentioning

confidence: 99%

Epidemiology of Methicillin-Resistant <i>Staphylococcus aureus</i>

Flückiger

Widmer

1999

Chemotherapy

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“…To enhance the reliability of such molecular laboratory results, the combined use of various genotyping methods appears to be effective [14,15]. An outbreak has been defined as infectious disease derived from the same pathogen, while an outbreak that originates from strains that are indistinguishable from each other by typing methods but for which no direct linkage can be demonstrated is called an ''endemic outbreak'' [16].…”

Section: Introductionmentioning

confidence: 99%

“…The goal of these laboratory tests is to provide firm evidence that isolates, which are epidemiologically related during an outbreak of the infection, are also genetically related and thus represent the same strain. To enhance the reliability of such molecular laboratory results, the combined use of various genotyping methods appears to be effective [14,15]. An outbreak has been defined as infectious disease derived from the same pathogen, while an outbreak that originates from strains that are indistinguishable from each other by typing methods but for which no direct linkage can be demonstrated is called an ''endemic outbreak'' [16].…”

Section: Introductionmentioning

confidence: 99%

Coincidental outbreak of methicillin‐resistant Staphylococcus aureus in a hematopoietic stem cell transplantation unit

Imataki¹,

Makimoto²,

Kato

et al. 2006

American J Hematol

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common nosocomial pathogens among hospital-acquired infections, and immunocompromised patients are highly susceptive to infection. The molecular typing of isolated strains is a common method for tracing an outbreak of MRSA, but experience with this approach is still limited in the hematopoietic stem cell transplantation (HSCT) ward.Methods: We experienced 6 cases of MRSA infection/colonization in our 26-bed HSCT ward during a 4-week period. This unusual outbreak strongly suggested that the same MRSA strain was involved despite strict isolation and aseptic patient care. Clarification of the transmission pattern was critical, and we applied pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) assays for evaluation.Results and conclusion: In four of the six cases, the pattern of bands examined by PFGE and AFLP analyses supported the idea that direct person-to-person transmission was very unlikely and the outbreak was coincidental. This experience highlights the clinical value of molecular typing methods for the clinical epidemiological assessment of MRSA outbreak.

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“…In addition to these, 16 staphylococcal isolates having both mecR1 and mecI were examined for the presence of IS431 downstream of mecA or mecI. S. aureus isolates were classified by coagulase type, coagulase gene type (13), and protein A type (15), and S. haemolyticus was discriminated by use of an arbitrarily primed PCR (AP-PCR) with ERIC2 and M13R primers (6,22).…”

mentioning

confidence: 99%

Genomic Rearrangement of the mec Regulator Region Mediated by Insertion of IS 431 in Methicillin-Resistant Staphylococci

Kobayashi

Alam

Urasawa

2001

Antimicrob Agents Chemother

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Genomic diversification of the mec regulator region mediated by IS431 was investigated for clinical isolates of methicillin-resistant staphylococci. A single rearranged form of the mecR1 gene due to IS431 insertion was detected in the three staphylococcal species, while another type of mecR1 truncation with IS431 and an IS431 located downstream of mecI were found only in Staphylococcus haemolyticus. Genetic differentiation of IS431 and staphylococcal isolates suggested transmission of mecDNA with IS431-mediated rearrangement among different staphylococcal species.

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Value of Molecular Epidemiologic Analysis in a Nosocomial Methicillin-Resistant Staphylococcus aureus Outbreak

Cited by 35 publications

References 38 publications

Epidemiology of Methicillin-Resistant <i>Staphylococcus aureus</i>

Epidemiology of Methicillin-Resistant <i>Staphylococcus aureus</i>

Coincidental outbreak of methicillin‐resistant Staphylococcus aureus in a hematopoietic stem cell transplantation unit

Genomic Rearrangement of the mec Regulator Region Mediated by Insertion of IS 431 in Methicillin-Resistant Staphylococci

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