Background: Applying of ventilator bundle of care according to evidence-based practice guidelines are expected to improve weaning, provide quality of care, improve patient outcomes, and reduce the risk of complications with ventilated patients. Aim: The researchers wanted to assess how effective a bundle of care was at weaning mechanically ventilated patients in the intensive care unit and cardiac care unit. Subjects and Method:The study used a quasi-experimental research design. A purposive sample of 60 mechanically ventilated patients at both insurance hospitals (Al-Salam) in Port Said governorate and health ministry hospital (Sherbin central) in Dakahlia governorate's critical care unit and cardiac care unit. Tools: three tools were used, first tool includes personal characteristics of the patients and, patient's medical history, second tool Is Ventilator bundle compliance checklist and the third one is Burns' Wean Assessment Program (BWAP) scores to assess and track weaning progresses of the mechanical ventilated patients. Results: This study revealed the improvement in weaning with ventilator bundle of care though out the ventilation days. Additionally, there was a statistically significant correlation between the studied patients' total Burns' weaning assessment and compliance with ventilator bundle of the studied patients with p-value <0.01. Conclusion: It may be concluded that mechanically ventilated patients who comply with the ventilator bundle of care have higher weaning ratings and spend less time on the ventilator. Consequently, the application of a ventilator bundle care is necessary for enhance weaning from mechanical ventilator in ICU and CCU patients.Recommendation: This study recommended applying the ventilator bundle practices for all mechanically ventilated patients with special focus on nursing performance. In addition, using a standardized approach to determine readiness for weaning which can aid in the management of mechanically ventilated patients. Moreover, the study's generalizability could be ensured by replication on a larger probability sample from different geographical areas in Egypt.