Value of histopathology in vitiligo

Montes, Leopold F.; Abulafia, J; Wilborn, Walter H.; Hyde, Barbara M.; Montes, Carolina M.

doi:10.1046/j.1365-4362.2003.01743.x

Cited by 51 publications

(54 citation statements)

References 36 publications

(25 reference statements)

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“…Finally according to our data, we suggest an involvement of this dendritic subset in the pathogenesis of vitiligo. Previous studies [12,14,17] have demonstrated an increase of Langerhans cells in vitiligo skin without clearing the subset involved. We have already shown in another skin disease [16] the relevance of dendritic cells in the pathogenesis, defying their role consistent with the numerous subsets.…”

Section: Discussionmentioning

confidence: 94%

“…As a lot of studies have provided contradictory results about the quantitative variation of Langerhans cells in the vitiligo-affected skin [12] and no previous data exist concerning the immunophenotypical distributions of specific markers of dendritic cells, we focused our attention on defining the ultrastructural features of lesional, perilesional, and non-lesional skin from vitiligo patients with a similar clinical history concerning the lesions' extension, duration of the disease, and absence of autoimmunity. We observed an increase of dendritic cells in lesional and perilesional vitiligo skin, with ultrastructural alterations in their mitochondria especially in perilesional skin [17].…”

Section: Introductionmentioning

confidence: 99%

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Dendritic cells: ultrastructural and immunophenotypical changes upon nb-UVB in vitiligo skin

et al. 2010

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The role of dendritic cells in vitiligo is still unclear. Few studies have provided contradictory results about their quantitative variation and no data exist concerning their immunophenotypical distribution in diseased skin. The purpose of our study was to analyze the presence, the distribution, the immunophenotypical markers and the effects of nb-UVB therapy on dendritic cells in nonlesional, perilesional, and lesional vitiligo skin. Punchbiopsies of 6 mm were taken from lesional, perilesional, and non-lesional skin of 12 patients affected by nonsegmental vitiligo, treated with nb-UVB. An immunohistochemical and an ultrastructural analysis were performed. Immunohistochemical and ultrastructural analysis showed both quantitative and qualitative modifications of Langerhans cells. Nb-UVB therapy, one of the most effective treatments for the disease, was able to reduce the Langerhans cells number and to redistribute main dendritic subsets. This study underlines the importance of dendritic cells, Langerhans cells in particular, in non-segmental vitiligo, in its pathogenesis and in its better therapeutical approach.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Dendritic cells: ultrastructural and immunophenotypical changes upon nb-UVB in vitiligo skin

et al. 2010

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“…Outras alterações também podem ser observadas, como dermatite de interface com infiltrado linfocitário na derme superficial (13) . Estas alterações são mais proeminentes no vitiligo ativo do que no estável (1,6) .…”

Section: Discussionunclassified

“…Alguns autores acreditam que melanócitos permaneçam no vitiligo, mas sem atividade melanogênica (4,10,15,19) , porém Montes et al (13) , por microscopia eletrônica, e Le Poole et al (6) , por imuno-histoquímica, demonstraram ausência de melanócitos nas áreas despigmentadas do vitiligo. Em nosso estudo, o caso 3 mostra na pele lesada células localizadas na camada basal da epiderme lembrando melanócitos, porém não imunomarcadas pelo HMB-45 (Figura 2B) nem pelo Melan-A; acreditamos que essas células correspondam a melanócitos, porém apoptóticos, e por isso não apresentam imunomarcação.…”

Section: Discussionunclassified

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Imuno-histoquímica para o diagnóstico precoce de vitiligo

Nai

Miot²,

Miot³

et al. 2008

J. Bras. Patol. Med. Lab.

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O vitiligo é uma doença de pele freqüente que acomete 1% da população e é caracterizada por máculas despigmentadas conseqüentes à perda progressiva e localizada dos melanócitos da epiderme. Na maioria dos pacientes, o diagnóstico é feito por exame clínico. A biópsia da pele é realizada quando há necessidade de diagnóstico diferencial com doenças hipocromiantes. O diagnóstico histopatológico de vitiligo é difícil nos preparados corados por hematoxilina e eosina (HE). Há poucos estudos sobre a melhoria da qualidade diagnóstica no vitiligo. Objetivo: Avaliar a utilidade dos marcadores imunohistoquímicos proteína S-100, human melanoma black-45 (HMB-45) e Melan-A para o diagnóstico precoce em casos clinicamente suspeitos ou duvidosos de vitiligo. Material e métodos: Lâminas histológicas de biópsias de pele sã e lesada de 10 pacientes com suspeita clínica de vitiligo coradas pelos métodos de HE, proteína S-100, HMB-45 e Melan-A. Utilizou-se contracoloração com Giemsa como modificação técnica para diferenciar a melanina da imunomarcação. Resultados: Seis casos, com manifestação clínica recente, apresentaram infiltrado linfocitário, do tipo dermatite de interface, na pele lesada na HE. As colorações por S-100, HMB-45 e Melan-A marcaram os melanócitos da camada basal da pele sã, e a proteína S-100 evidenciou as células de Langerhans. Na pele lesada, os melanócitos estavam ausentes ou diminuídos quando comparados com a pele normal. A proteína S-100 demonstrou maior número de células de Langerhans, o que é característico das lesões de vitiligo. Conclusão: A imuno-histoquímica pode ser utilizada como método auxiliar no diagnóstico dos casos duvidosos de vitiligo. abstract resumo Vitiligo is a frequent skin disease that affects 1% of the population. It presents depigmented macules resulting from a gradual loss of melanocytes in the epidermis. In most cases, the diagnosis is made by clinical examination. Skin biopsies are performed when it is necessary to compare it with other hypomelanosis. Histopathological diagnosis of vitiligo is often difficult in hematoxylin-eosin (H&E

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Disorganisation of basement membrane zone architecture impairs melanocyte residence in vitiligo

Yang,

Kuroda

et al. 2024

The Journal of Pathology

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The basement membrane zone is the interface between the epidermis and dermis, and it is disrupted in several skin conditions. Here, we report the results of a comprehensive investigation into the structural and molecular factors of the basement membrane zone in vitiligo, a dermatological disorder characterised by depigmented patches on the skin. Using electron microscopy and immunofluorescence staining, we confirmed abnormal basement membrane zone morphology and disrupted basement membrane zone architecture in human vitiliginous skin. Furthermore, we identified elevated expression of matrix metalloproteinase 2 (MMP2) in human dermal fibroblasts as a key factor responsible for basement membrane zone matrix degradation. In our in vitro and ex vivo models, overexpression of MMP2 in fibroblasts led to basement membrane zone disruption and melanocyte disappearance. Importantly, we reveal that the loss of melanocytes in vitiligo is primarily linked to their weakened adhesion to the basement membrane, mediated by binding between integrin β1 and laminin and discoidin domain receptor 1 and collagen IV. Finally, inhibition of matrix metalloproteinase 2 expression reversed depigmentation in a mouse model of vitiligo. In conclusion, our research shows the importance of basement membrane zone integrity in melanocyte residence and offers new avenues for therapeutic interventions to address this challenging skin condition. © 2024 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.

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Value of histopathology in vitiligo

Cited by 51 publications

References 36 publications

Dendritic cells: ultrastructural and immunophenotypical changes upon nb-UVB in vitiligo skin

Dendritic cells: ultrastructural and immunophenotypical changes upon nb-UVB in vitiligo skin

Imuno-histoquímica para o diagnóstico precoce de vitiligo

Disorganisation of basement membrane zone architecture impairs melanocyte residence in vitiligo

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