Valorizing municipal organic waste to produce biodiesel, biogas, organic fertilizer, and value-added chemicals: an integrated biorefinery approach

Sousa, Maria Helena de; Silva, Alice Sabrina Ferreira da; Correia, Raphael Chaves; Leite, Nathalia Pereira; Bueno, Claudio Eduardo Gonçalves; Pinheiro, Ricardo Luís dos Santos; Santana, Juliana Silva de; Silva, Juliana Luna da; Sales, Aldo Torres; Souza, Camila Claudino de; Aquino, Kátia Aparecida da Silva; Souza, Rafael Barros de; Pinheiro, Irapuan Oliveira; Guerrero, Jorge Recarte Henriquez; Schuler, Alexandre Ricardo Pereira; Sampaio, Everardo Valadares de Sá Barretto; Dutra, Emmanuel Damilano; Menezes, Rômulo Simões Cézar

doi:10.1007/s13399-020-01252-5

Cited by 18 publications

(7 citation statements)

References 64 publications

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“…Chemicals, microorganism, culture media and crude enzyme extracts production Raw glycerol was obtained by separation of biodiesel after chemical transesteri cation of waste cooking oil and sodium metoxide [35]. It was sterilized and directly supplied as carbon source in a basal medium for fungal growth.…”

Section: Methodsmentioning

confidence: 99%

Production of a β-glucosidase-Rich Cocktail from Talaromyces Amestolkiae using Raw Glycerol: Its Role for Lignocellulose Wastes Valorization

Méndez-Líter

Eugenio

Hakalin

et al. 2021

Preprint

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Background: As β-glucosidases represent the major bottleneck for industrial degradation of plant biomass, great efforts are being devoted both to discover novel and robust versions of these enzymes, as well as to develop efficient and inexpensive ways to produce them. In this work, raw glycerol from chemical production of biodiesel was tested as carbon source for the fungus Talaromyces amestolkiae with the aim of producing enzyme cocktails rich in this activity. Results: When using raw glycerol as sole carbon source, approximately 11 U/mL β-glucosidase were detected in these cultures, constituting the major cellulolytic activity. Besides, it was detected that the enzymatic production started when glycerol was completely depleted, which implicates that it was produced under carbon starvation stimuli. Proteomic analysis of the produced crudes revealed BGL-3 as the most abundant protein and the main b-glucosidase. This enzymatic cocktail was successfully used to supplement a basal commercial cellulolytic cocktail (Celluclast 1.5L) for saccharification of different pretreated wheat straw, and improving the yield that the commercial preparation can reach alone.Conclusions: This study corroborates that even hardly exploitable industrial wastes, such as glycerol, can be used by Talaromyces amestolkiae as carbon sources to produce very valuable enzymatic preparations for the production of biofuels and other bioproducts in a framework of circular economy.

show abstract

Section: Methodsmentioning

confidence: 99%

Production of a β-glucosidase-Rich Cocktail from Talaromyces Amestolkiae using Raw Glycerol: Its Role for Lignocellulose Wastes Valorization

Méndez-Líter

Eugenio

Hakalin

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Raw glycerol was obtained by separation of biodiesel after chemical transesterification of waste cooking oil and sodium metoxide [12]. It was directly supplied as carbon source in a basal medium for fungal growth.…”

Section: Chemicals Microorganisms Culture Media and Crude Enzyme Extract Productionmentioning

confidence: 99%

Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization

Méndez-Líter

Eugenio

Hakalin

et al. 2021

JoF

View full text Add to dashboard Cite

As β-glucosidases represent the major bottleneck for the industrial degradation of plant biomass, great efforts are being devoted to discover both novel and robust versions of these enzymes, as well as to develop efficient and inexpensive ways to produce them. In this work, raw glycerol from chemical production of biodiesel was tested as carbon source for the fungus Talaromyces amestolkiae with the aim of producing enzyme β-glucosidase-enriched cocktails. Approximately 11 U/mL β-glucosidase was detected in these cultures, constituting the major cellulolytic activity. Proteomic analysis showed BGL-3 as the most abundant protein and the main β-glucosidase. This crude enzyme was successfully used to supplement a basal commercial cellulolytic cocktail (Celluclast 1.5 L) for saccharification of pretreated wheat straw, corroborating that even hardly exploitable industrial wastes, such as glycerol, can be used as secondary raw materials to produce valuable enzymatic preparations in a framework of the circular economy.

show abstract

“…Raw glycerol was obtained by separation of biodiesel after chemical transesterification of waste cooking oil and sodium metoxide [35]. It was sterilized and directly supplied as carbon source in a basal medium for fungal growth.…”

Section: Chemicals Microorganism Culture Media and Crude Enzyme Extracts Productionmentioning

confidence: 99%

Production of a β-Glucosidase-Rich Cocktail From Talaromyces Amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Wastes Valorization

Méndez-Líter¹,

Eugenio²,

Hakalin³

et al. 2021

Preprint

View full text Add to dashboard Cite

As β-glucosidases represent the major bottleneck for industrial degradation of plant biomass, great efforts are being devoted both to discover novel and robust versions of these enzymes, as well as to develop efficient and inexpensive ways to produce them. In this work, raw glycerol from chemical production of biodiesel was tested as carbon source for the fungus Talaromyces amestolkiae with the aim of producing enzyme cocktails rich in this activity. Approximately 11 U/mL β-glucosidase were detected in these cultures, constituting the major cellulolytic activity. Proteomic analysis revealed BGL-3 as the most abundant protein and the main β-glucosidase. This enzyme crude was successfully used to supplement a basal commercial cellulolytic cocktail (Cellu-clast 1.5L) for saccharification of pretreated wheat straw, corroborating that even hardly exploitable industrial wastes, such as glycerol, can be used as secondary raw materials to produce valuable enzymatic preparations in a framework of circular economy

show abstract

Valorizing municipal organic waste to produce biodiesel, biogas, organic fertilizer, and value-added chemicals: an integrated biorefinery approach

Cited by 18 publications

References 64 publications

Production of a β-glucosidase-Rich Cocktail from Talaromyces Amestolkiae using Raw Glycerol: Its Role for Lignocellulose Wastes Valorization

Production of a β-glucosidase-Rich Cocktail from Talaromyces Amestolkiae using Raw Glycerol: Its Role for Lignocellulose Wastes Valorization

Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization

Production of a β-Glucosidase-Rich Cocktail From Talaromyces Amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Wastes Valorization

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