Valorization of sugar beet molasses for the production of biohydrogen and 5-aminolevulinic acid by Rhodobacter sphaeroides O.U.001 in a biorefinery concept

Kars, Gökhan; Alparslan, Ümmühan

doi:10.1016/j.ijhydene.2013.09.050

Cited by 32 publications

(37 citation statements)

References 28 publications

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“…It is the viscous dark brown by-product of the refining of sugar beets and contains 50% (w/w) sugar, mainly sucrose. Sugar-containing medium with sucrose concentration of 28 g/L was used based on our previous results [ 11 ] that in this medium, 5-ALA (35 mg/g dry cell weight (DCW)) and H 2 (1.01 L/L culture) can be obtained using molasses. Fifty-five milliliter glass bioreactors with a final culture volume of 50 mL were used.…”

Section: Methodsmentioning

confidence: 99%

“…In addition to 2 mmol/L of glutamate added as a nitrogen source, K 2 HPO 4 , MgSO 4 ·7H 2 O, CaCl 2 ·2H 2 O, FeSO 4 , MoO 4 ·2H 2 O and vitamin solution (Thiamine, Niacin, Biotin) were included in the culture medium as described earlier. [ 11 ] After 10% inoculation, the anaerobic cultures were incubated under the conditions defined above. During the bioprocess (circa the 90th hour), levulinic acid (1.74 g/L, 15 mmol/L) was added into the cultures to enhance 5-ALA production, as recommended by Choi et al.…”

Section: Methodsmentioning

confidence: 99%

“…The evolved gas was collected in water-filled graduated tubes during the bioprocess and then the purity was determined at the end of the bioprocesses by gas chromatography (Shimadzu GC-2010 Plus, Japan) as described previously. [ 11 ] The amount of hydrogen accumulation was given as the liters of hydrogen produced per liter of culture (L H 2 /L culture) and as the yield (mol H 2 /mol sucrose), which was calculated by taking the temperature to be 29 ºC and the pressure to be 101.3 kPa (i.e. 1 atm).…”

Section: Methodsmentioning

confidence: 99%

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Heterologous expression of glutamyl-tRNA reductase gene inRhodobacter sphaeroidesO.U.001 to enhance 5-aminolevulinic acid production

Kars

Alparslan

2014

Biotechnology & Biotechnological Equipment

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The pathways for synthesis of 5-aminolevulinic acid (5-ALA) use either succinyl-CoA and glycine (C-4 pathway), or glutamate (C-5 pathway). Although Rhodobacter sphaeroides synthesizes 5-ALA through the C-4 pathway, it also has the genes coding for the enzymes of the C-5 pathway, except for glutamyl-tRNA reductase. The glutamyl-tRNA reductase gene was cloned from Rhodospirillum rubrum and expressed in R. sphaeroides; thus, the C-5 pathway was enabled to function upon assembling all the required genes. Consequently, a new and unique bacterial strain producing more 5-ALA was developed. Biohydrogen was also produced in the same bioprocess within a biorefinery approach using sugar beet molasses as substrate. The amount of 5-ALA produced by the modified strain was 25.9 mg/g dry cell weight (DCW), whereas the wild-type strain produced 12.4 mg/g DCW. In addition, the amount of H2 generated by the modified and wild-type cells, respectively, was 0.92 L/L culture and 1.05 L/L culture.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Heterologous expression of glutamyl-tRNA reductase gene inRhodobacter sphaeroidesO.U.001 to enhance 5-aminolevulinic acid production

Kars

Alparslan

2014

Biotechnology & Biotechnological Equipment

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“…As the process of fermentation involves the production of several intermediates [100], as do many other metabolic processes, many recent advances in sugar valorisation utilised fermentation to other target molecules, particularly platform molecules-those identified as being a set of basic chemical building blocks for synthesis into a wider range of molecules. Notable ones include lactic acid [101,102], succinic acid, and levulinic acid [103]-structures shown in Fig. 2-although more will be covered throughout this review.…”

Section: Sugarsmentioning

confidence: 99%

Valorisation of Biowastes for the Production of Green Materials Using Chemical Methods

Dugmore

Clark

Bustamante

et al. 2017

Topics in Current Chemistry Collections

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With crude oil reserves dwindling, the hunt for a sustainable alternative feedstock for fuels and materials for our society continues to expand. The biorefinery concept has enjoyed both a surge in popularity and also vocal opposition to the idea of diverting food-grade land and crops for this purpose. The idea of using the inevitable wastes arising from biomass processing, particularly farming and food production, is, therefore, gaining more attention as the feedstock for the biorefinery. For the three main components of biomass-carbohydrates, lipids, and proteins-there are longestablished processes for using some of these by-products. However, the recent advances in chemical technologies are expanding both the feedstocks available for processing and the products that be obtained. Herein, this review presents some of the more recent developments in processing these molecules for green materials, as well as case studies that bring these technologies and materials together into final products for applied usage.

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“…Özgür and Peksel 10 realized photobiological hydrogen production on barley straw dark‐fermented effluent. Kars and Alparslan 11 presented new findings on the enhanced accumulation of biohydrogen. Cai et al 12 optimized the culture conditions to increase the production of hydrogen in batch cultivations using butyrate as the carbon source.…”

Section: Introductionmentioning

confidence: 99%

Hydrogen Production by a Photosynthetic Bacterium: Some Analytical Solutions

Rasi¹,

Rajendran²

2015

Chem Eng & Technol

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The mathematical model for hydrogen production by the photosynthetic bacterium Rhodobacter capsulatus in a batch photobioreactor is discussed. An analytical method -the homotopy perturbation method -is presented to solve the nonlinear differential equations that describe the biomass formation, substrate utilization, and hydrogen production with respect to time. Approximate analytical expressions for the concentrations of biomass, substrate and hydrogen are derived for various values of the relevant parameters. The analytical results are compared with experimental data. In addition, the sensitivity of the kinetic parameters is also analyzed. The time required to achieve the maximum growth of biomass and complete degradation of the substrate is reported.

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Valorization of sugar beet molasses for the production of biohydrogen and 5-aminolevulinic acid by Rhodobacter sphaeroides O.U.001 in a biorefinery concept

Cited by 32 publications

References 28 publications

Heterologous expression of glutamyl-tRNA reductase gene inRhodobacter sphaeroidesO.U.001 to enhance 5-aminolevulinic acid production

Heterologous expression of glutamyl-tRNA reductase gene inRhodobacter sphaeroidesO.U.001 to enhance 5-aminolevulinic acid production

Valorisation of Biowastes for the Production of Green Materials Using Chemical Methods

Hydrogen Production by a Photosynthetic Bacterium: Some Analytical Solutions

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