Valorization of olive stones for xylitol and ethanol production from dilute acid pretreatment via enzymatic hydrolysis and fermentation by Pachysolen tannophilus

Saleh, Marwa; Cuevas, Manuel Bruña; García, J.; Sánchez, Sebastián

doi:10.1016/j.bej.2014.06.023

Cited by 44 publications

(41 citation statements)

References 32 publications

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“…According to RMS outputs, 20.4 g D-xylose can be obtained from 100 g olive stones (87.2% D-xylose extraction) under the most favorable conditions (50 g solid loading, 0.016 M H 2 SO 4 ). This yield is comparable to those achieved by Saleh et al (2014) and Cuevas et al (2013). Saleh et al (2014) by the synergistic action of hemicellulases and β-glucosidases on pre-treated olive stones by autohydrolysis.…”

Section: Hydrolysate Composition: Regression and Model Analysissupporting

confidence: 79%

“…The preliminary maximum D-xylose yield achieved by dilute sulphuric acid hydrolysis (18.1%) was a promising result, since the potential yield in raw material was 23.4% (77.4% D-xylose extraction). Nevertheless, this result was lower than the maximum D-xylose extraction (89.7%) from olive stones by dilute sulphuric acid hydrolysis reported by Saleh et al (2014). What is more, full D-xylose extraction without degradation has been reported by Cuevas et al (2013) by optimizing the enzymatic hydrolysis (using a mixture of hemicellulases and β-glucosidases) of pretreated olive stones by autohydrolysis.…”

Section: Characterization Of Hydrolysatescontrasting

confidence: 43%

“…Cuevas et al (2013) achieved full D-xylose recovery from hemicellulose by means of enzymatic hydrolysis preceded by autohydrolysis. By contrast, Saleh et al (2014) aimed for D-xylose recovery in one single step without the need of resorting to the use of enzymes, which enhances the feasibility of the process. By using sulphuric acid as a hydrolytic agent, these authors (Saleh et al, 2014) recovered 89.7% of the available D-xylose in olive stones.…”

Section: Introductionmentioning

confidence: 99%

“…Furfural production has also been assayed with dilute acid hydrolysis as it is an important renewable, non-petroleum based, chemical feedstock (Montané et al, 2002). Only Cuevas et al (2013) and Saleh et al (2014) have previously attempted the recovery of D-xylose from olive stones. Cuevas et al (2013) achieved full D-xylose recovery from hemicellulose by means of enzymatic hydrolysis preceded by autohydrolysis.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, these authors Saleh et al, 2014) assayed a relatively low solid/liquid ratio in the reactor (1/6). The use of higher olive stone loadings has not been assayed.…”

Section: Introductionmentioning

confidence: 99%

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Influence of solid loading on D-xylose production through dilute sulphuric acid hydrolysis of olive stones

Cuevas¹,

Saleh²,

García‐Martín³

et al. 2015

Grasas y Aceites

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SUMMARY:The selective hydrolysis of hemicellulose from olive stones was attempted in order to achieve a maximum D-xylose yield. For this aim, batch hydrolysis was conducted under different operating conditions of temperature, acid concentration and solid loading. Firstly, distilled water, sulphuric acid and nitric acid were assessed as hydrolytic agents at different temperatures (200, 205, 210 and 220 °C) and at a fixed acid concentration (0.025 M). Sulphuric acid and 200 °C were selected for the subsequent dilute acid hydrolysis optimization based on the obtained D-xylose yields. The combined influence of solid loading (from 29.3 to 170.7 g olive stones into 300 mL acid solution) and sulphuric acid concentration (0.006-0.034 M) on the release of D-xylose was then estimated by response surface methodology. According to a statistical analysis, both parameters had significant interaction effects on D-xylose production. The results illustrated that the higher the solid loading, the higher the required acid concentration. The decrease in the solid/liquid ratio in the reactor had a positive effect on D-xylose extraction and on the amount of acid used. The optimum solid loading and sulphuric acid concentration were determined to be 50 g (solid/liquid ratio 1/6) and 0.016 M, respectively. Under these conditions, the predicted D-xylose yield (expressed as g of sugar per 100 g of dry matter fed) was 20.4 (87.2% of maximum attainable). KEYWORDS: D-xylose; Dilute acid hydrolysis; Hemicellulose; Olive stones; Response surface methodologyRESUMEN: Influencia de la carga de sólido en la producción de D-xilosa mediante hidrólisis del hueso de aceituna con ácido sulfúrico diluido. Se ha desarrollado una hidrólisis selectiva de la fracción hemicelulósica del hueso de aceituna con el fin de obtener el máximo rendimiento de D-xilosa. Para ello las hidrólisis se llevaron a cabo en un reactor discontinuo a distintas condiciones de temperatura, concentración de ácido y carga de sólidos. En primer lugar se evaluó la capacidad hidrolítica del agua destilada y de los ácidos nítrico y sulfúrico a distintas temperaturas (200, 205, 210 y 220°C) manteniendo fija la concentración de ácido (0,025 M). A partir de los rendimientos en D-xilosa obtenidos se seleccionó el ácido sulfúrico y una temperatura de 200 °C para la posterior optimización del proceso. La influencia conjunta de la carga de sólidos (desde 29,3 a 170,7 g de hueso de aceituna en 300 mL de disolución ácida) y de la concentración de ácido sulfúrico (0,006-0,034 M) sobre los rendimientos de D-xilosa fue determinada mediante metodología de superficie de respuesta. Según el análisis estadístico ambos parámetros tuvieron un efecto interactivo significativo en la producción de D-xilosa. Los resultados mostraron que se requiere mayor concentración de ácido a mayor carga de sólidos. La disminución de la relación sólido/líquido en el reactor tuvo un efecto positivo en la extracción de D-xilosa y en la cantidad de ácido utilizado. Las condiciones óptimas fueron 50 g de sólidos (relación ...

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Section: Hydrolysate Composition: Regression and Model Analysissupporting

confidence: 79%

Section: Characterization Of Hydrolysatescontrasting

confidence: 43%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, these authors Saleh et al, 2014) assayed a relatively low solid/liquid ratio in the reactor (1/6). The use of higher olive stone loadings has not been assayed.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Influence of solid loading on D-xylose production through dilute sulphuric acid hydrolysis of olive stones

Cuevas¹,

Saleh²,

García‐Martín³

et al. 2015

Grasas y Aceites

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Fermentation of hemicellulose liquor from Brewer's spent grain using Scheffersomyces stipitis and Pachysolen tannophilus for production of 2G ethanol and xylitol

Silva

Borges

Maione

et al. 2019

Biofuels Bioprod Bioref

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Beer production generates brewer's spent grain as a byproduct. This biomass contains lignin, cellulose, and hemicellulose, and can be used for the production of second‐generation (2G) ethanol and xylitol. For this to be economically viable, fermentable fractions must be used, allowing the conversion of cellulose and hemicellulose to ethanol. This study evaluated the fermentation of hemicellulose liquor from brewer's spent grain (HLBS) in aerobic and oxygen‐ limited conditions using Scheffersomyces stipitis and Pachysolen tannophilus. Results were compared with data obtained in a complex medium (YPX) and a minimal medium (MMX). In aerobic conditions, for S. stipitis in the HLBS medium, μ max = 0.12 h−1, Y ethanol/xylose = 0.14 and selectivity for ethanol over xylitol (S ethanol/xylitol) 3.4 gethanol g−1 xylitol were obtained. Under oxygen‐limited conditions, Y ethanol/xylose = 0.22 and S ethanol/xylitol = 1.17 gethanol g−1 xylitol were obtained. For P. tannophilus, under aerobic conditions, the HLBS medium gave μ max = 0.08 h−1, and Y ethanol/xylose = 0.005, with selectivity for ethanol over xylitol (S ethanol/xylitol) of 0.12 gethanol g−1 xylitol. In oxygen‐limited conditions, Y ethanol/xylose = 0.09 and S ethanol/xylitol = 0.20 gethanol g−1 xylitol were obtained. This is because the metabolism of these aerobic yeasts directs the carbon flow to cell growth, while with oxygen limitation it is directed to metabolite production. The HLBS medium was promising for production in the context of a biorefinery. When comparing the two yeasts, S. stipitis presented better ethanol production and P. tannophilus better xylitol production under the conditions that were studied. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd

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Bioactive Phytochemicals from Olive (Olea europaea) Processing By-products

Delgado

Chammem

Issaoui

et al. 2022

Reference Series in Phytochemistry

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Valorization of olive stones for xylitol and ethanol production from dilute acid pretreatment via enzymatic hydrolysis and fermentation by Pachysolen tannophilus

Cited by 44 publications

References 32 publications

Influence of solid loading on D-xylose production through dilute sulphuric acid hydrolysis of olive stones

Influence of solid loading on D-xylose production through dilute sulphuric acid hydrolysis of olive stones

Fermentation of hemicellulose liquor from Brewer's spent grain using Scheffersomyces stipitis and Pachysolen tannophilus for production of 2G ethanol and xylitol

Bioactive Phytochemicals from Olive (Olea europaea) Processing By-products

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