“…The main composition of RBPC was protein with 68.07 AE 0.54% dm, which can be labeled as protein concentrate due to the protein content of more than 60% [58]. In addition, two step statistical design (1) fractional factorial design 2 4-1 (independent variables: X 1 (pH: 8,9,10), X 2 (temperature: 25, 35, and 45°C), X 3 (stirring speed: 80, 100, and 120 rpm) and X4 (stirring time: 60, 120, and 180 min) and (2) central composite rotatable design with variables X 1 (temperature: 35, 38, 45, 52, and 55°C) and X 2 (stirring time: 120, 146, 210, 274, and 300 min) was used to extract rice bran protein [57]. However, with this experimental design provided only 48.53% of protein content at pH 10.0, 80 rpm, 300 min of stirring time, and 52°C, with an extraction yield of 34.51%.…”