Valorization of chicken feather waste using recombinant bacillus subtilis cells by solid-state fermentation for soluble proteins and serine alkaline protease production

El Salamony, Dina.H.; Salah Eldin Hassouna, Mohamed; Zaghloul, Taha.I.; Moustafa Abdallah, Hanan

doi:10.1016/j.biortech.2023.130110

Cited by 9 publications

(1 citation statement)

References 34 publications

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“…Pullulanase PulA was co-expressed to produce the recombinant strain B. subtilis PSH02, which achieved 80% trehalose conversion at 100 g/L maltodextrin [72]. Recombinant B. subtilis DB100, carrying a pUB110-derived plasmid with an alkaline serine protease with keratinolytic activity, has been used for solid-state fermentation (SSF) of feather waste, which is 91% β-keratin and thus a valuable by-product [73].…”

Section: Enzymesmentioning

confidence: 99%

Cloning Systems in Bacillus: Bioengineering of Metabolic Pathways for Valuable Recombinant Products

Arsov,

Armenova,

Gergov

et al. 2024

Fermentation

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Representatives of the genus Bacillus have been established as one of the most important industrial microorganisms in the last few decades. Genetically modified B. subtilis and, to a lesser extent, B. licheniformis, B. amyloliquefaciens, and B. megaterium have been used for the heterologous expression of numerous proteins (enzymes, vaccine components, growth factors), platform chemicals, and other organic compounds of industrial importance. Vectors designed to work in Bacillus spp. have dramatically increased in number and complexity. Today, they provide opportunities for genetic manipulation on every level, from point mutations to systems biology, that were impossible even ten years ago. The present review aims to describe concisely the latest developments in the shuttle, integrative, and CRISPR-Cas9 vectors in Bacillus spp. as well as their application for large-scale bioengineering with the prospect of producing valuable compounds on an industrial scale. Genetic manipulations of promoters and vectors, together with their impact on secretory and metabolic pathways, are discussed in detail.

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Section: Enzymesmentioning

confidence: 99%