Valine promotes milk synthesis by regulating PKM2 nuclear accumulation and histone H3 acetylation through the TAS1R1-mTOR-DDX39B signaling pathway

Cao, Yu; Hu, Guiqiu; Long, Xiaoyu; Li, Feng; Wang, Jiaxin; Sun, Mingyang; Xie, Yachun; Ge, Yusong; Guo, Wenjin; Liu, Juxiong; Fu, Shoupeng

doi:10.1016/j.ijbiomac.2023.127786

Cited by 1 publication

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Heilongjiang Bayi Agricultural University's Animal Nutrition and Metabolic Disease Laboratory and Animal Population Common Disease Monitoring Laboratory donated mammary tissue of dairy cows. 22 Cell Culture and Treatment. The tissues of the mammary gland were collected for the isolation of BMECs.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…After cooling to room temperature, the sections were washed twice with PBS for 5 min each. 22 After cleaning, the segments were exposed to a peroxidase inhibitor for 10 min at ambient temperature. After blocking, the sections were treated with 5% goat serum for 2 h at room temperature.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Subsequently, the sections were deparaffinized, rehydrated, and subjected to antigen retrieval using citrate buffer. After cooling to room temperature, the sections were washed twice with PBS for 5 min each . After cleaning, the segments were exposed to a peroxidase inhibitor for 10 min at ambient temperature.…”

Section: Methodsmentioning

confidence: 99%

“…Heilongjiang Bayi Agricultural University’s Animal Nutrition and Metabolic Disease Laboratory and Animal Population Common Disease Monitoring Laboratory donated mammary tissue of dairy cows …”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Stigmasterol Activates the mTOR Signaling Pathway by Inhibiting ORP5 Ubiquitination to Promote Milk Synthesis in Bovine Mammary Epithelial Cells

Sun,

Cao,

Cheng

et al. 2024

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Stigmasterol (ST), a phytosterol found in food, has various biological activities. However, the effect of ST on milk synthesis in dairy cows remains unclear. Therefore, bovine primary mammary epithelial cells (BMECs) were isolated, cultured, and treated with ST to determine the effect of ST on milk synthesis. The study revealed that 10 μM ST significantly increased milk synthesis in BMECs by activating the mammalian target of rapamycin (mTOR) signaling pathway. Further investigation revealed that this activation depends on the regulatory role of oxysterol binding protein 5 (ORP5). ST induces the translocation of ORP5 from the cytoplasm to the lysosome, interacts with the mTOR, recruits mTOR to target the lysosomal surface, and promotes the activation of the mTOR signaling pathway. Moreover, ST was found to increase ORP5 protein levels by inhibiting its degradation via the ubiquitin-proteasome pathway. Specifically, the E3 ubiquitin ligase membrane-associated cycle-CH-type finger 4 (MARCH4) promotes the ubiquitination and subsequent degradation of ORP5. ST mitigates the interaction between MARCH4 and ORP5, thereby enhancing the structural stability of ORP5 and reducing its ubiquitination. In summary, ST stabilizes ORP5 by inhibiting the interaction between MARCH4 and ORP5, thereby activating mTOR signaling pathway and enhancing milk synthesis.

show abstract

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations