Validity of the microdialysis technique for experimental <i>in vivo</i> studies of myocardial energy metabolism

Kavianipour, Mohammad; Wikström, Gerhard; Ronquist, Gunnar; Waldenström, Anders

doi:10.1046/j.1365-201x.2003.01145.x

Cited by 15 publications

(21 citation statements)

References 21 publications

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“…All animals underwent a 45-min index ischaemic insult, as described previously (Kavianipour et al, 2003). Preconditioning was carried out using four episodes of 10 min of ischaemia, induced by drawing the snare and occluding the LAD branch, each followed by 20 min of reperfusion.…”

Section: Experimental Protocolmentioning

confidence: 99%

Ischaemic preconditioning reduces myocardial calcium overload in coronary‐occluded pig hearts shown by continuous in vivo assessment using microdialysis

Waldenström

Ronquist

Åberg

et al. 2011

Clin Physio Funct Imaging

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During ischaemia, ATP depletion leads to insufficient fuelling for Na(+) /K(+) ATPase, decreased electrochemical potential and increased influx of calcium ions. This study demonstrated a means to assess the effects of ischaemic preconditioning (IP) on the free intracellular Ca(2+) pool during prolonged ischaemia. In a porcine myocardial ischaemia model, microdialysis (MD) was used for sampling of metabolic and injury markers in IP and non-IP (control) groups. (45) Ca(2+) was delivered in microperfusate locally to ischaemic myocardium, with distribution and uptake assessed by (45) Ca(2+) recovery in microdialysate. Cardiomyocytes in vitro were exposed to a Ca(2+) ionophore and tested for (45) Ca(2+) uptake. An accentuated myocardial calcium ion influx (observed as an increased microdialysate (45) Ca(2+) recovery in the extracellular milieu) was noted in control pigs compared with IP pigs during ischaemia. Suspended cardiomyocytes preincubated with a Ca(2+) ionophore to increase the intracellular calcium ion pool and subsequently incubated with (45) Ca(2+) , displayed lower (45) Ca(2+) uptake in cells compared with control cells not exposed to the ionophore, corroborating the idea of a strong relationship between degree of intracellular calcium overload and microdialysate (45) Ca(2+) recovery. The ischaemic insult was differentially verified by metabolic and injury markers. We introduce an in vivo method for serial assessment of myocardial calcium overload during ischaemia, using a MD technique and (45) Ca(2+) inclusion. IP leads to relatively less calcium overload as assessed by this new method, and we interpret this to mean that reduction in calcium overload is an important part of the IP protective effect.

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Section: Experimental Protocolmentioning

confidence: 99%

Ischaemic preconditioning reduces myocardial calcium overload in coronary‐occluded pig hearts shown by continuous in vivo assessment using microdialysis

Waldenström

Ronquist

Åberg

et al. 2011

Clin Physio Funct Imaging

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“…Microdialysis sampling can be performed in organs which move, and this technique has been used to study metabolic aspects of the beating heart (Kavianipour et al. , 2003; Waldenström et al.…”

Section: Introductionmentioning

confidence: 99%

“…There is risk for damage to heart tissue as well as damage to the probe when sharply inserting the microdialysis probe (Mantovani et al. , 2002; Kavianipour et al. , 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Microdialysis facilitates serial biochemical sampling through a probe which lies within the substance of an organ. Serial sample collection is based on passive diffusion through a semi-permeable membrane placed at the end of the sampling probe (Ungerstedt, 1991;Chaurasia, 1999;Stahl et al, 2002;Kavianipour et al, 2003;Waldenström et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Microdialysis sampling can be performed in organs which move, and this technique has been used to study metabolic aspects of the beating heart (Kavianipour et al, 2003;Waldenström et al, 2010). How and exactly where the membrane is situated is not easy to determine since it lies in the myocardium and the membrane can no longer be visualized.…”

Section: Introductionmentioning

confidence: 99%

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Detection of myocardial ischaemia using surface microdialysis on the beating heart

Abrahamsson

Åberg

Johansson

et al. 2010

Clinical Physiology and Functional Imaging

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SummaryMicrodialysis (MD) can be used to study metabolism of the beating heart. We investigated whether microdialysis results obtained from epicardial (surface) sampling reflect acute changes in the same way as myocardial sampling from within the substance of the ventricular wall. In anaesthetized open-thorax pigs a coronary snare was placed. One microdialysis probe was placed with the sampling membrane intramyocardially (myocardial), and a second probe was placed with the sampling membrane epicardially (surface), both in the area which was made ischaemic. Ten minutes collection intervals were used for microdialysis samples. Samples from 19 pigs were analysed for lactate, glucose, pyruvate and glycerol during equilibration, baseline, ischaemia and reperfusion periods. For both probes (surface and myocardial), a total of 475 paired simultaneous samples were analysed. Results from analyses showed no differences in relative changes for glucose, lactate and glycerol during baseline, ischaemia and reperfusion. Surface microdialysis sampling is a new application of the microdialysis technique that shows promise and should be further studied.

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Continuous Monitoring of Organ Chemistry — a Paradigm Shift in Management of Intensive Care

Ungerstedt

Anaesthesia, Pain, Intensive Care and Emergency A.P.I.C.E.

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Validity of the microdialysis technique for experimental in vivo studies of myocardial energy metabolism

Cited by 15 publications

References 21 publications

Ischaemic preconditioning reduces myocardial calcium overload in coronary‐occluded pig hearts shown by continuous in vivo assessment using microdialysis

Ischaemic preconditioning reduces myocardial calcium overload in coronary‐occluded pig hearts shown by continuous in vivo assessment using microdialysis

Detection of myocardial ischaemia using surface microdialysis on the beating heart

Continuous Monitoring of Organ Chemistry — a Paradigm Shift in Management of Intensive Care

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