Validity of photo-oxidative stress markers and stress-related phytohormones as predictive proxies of mortality risk in the perennial herb Plantago lanceolata

Morales, Manuel Poblano; Roach, Deborah A.; Quarles, Brandie M.; Cotado, Alba; Salguero‐Gómez, Roberto; Dwyer, John M.; Munné‐Bosch, Sergi

doi:10.1016/j.envexpbot.2021.104598

Cited by 11 publications

(3 citation statements)

References 59 publications

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“…In the present study, it was found the changes in AgMYB5 expression and β-carotene content were closely related in celery, and ectopic expression of AgMYB5 in A. thaliana promoted β-carotene accumulation and up-regulated β-carotene synthesis-related genes in a water-deprived environment, which confirmed the involvement of AgMYB5 in β-carotene biosynthesis under drought stress. At the same time, β-carotene, an excellent natural antioxidant, confers stress resistance to plants by scavenging excess ROS [ 52 ]. In Dioscorea esculenta , the over-production of β-carotene led to a stronger DPPH radical-scavenging capacity and a higher photosynthetic efficiency [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

AgMYB5, an MYB transcription factor from celery, enhanced β-carotene synthesis and promoted drought tolerance in transgenic Arabidopsis

Sun

Zhu

et al. 2023

BMC Plant Biol

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Background Water shortage caused by global warming seriously affects the yield and quality of vegetable crops. β-carotene, the lipid-soluble natural product with important pharmacological value, is abundant in celery. Transcription factor MYB family extensively disperses in plants and plays regulatory roles in carotenoid metabolism and water scarcity response. Results Here, the AgMYB5 gene encoding 196 amino acids was amplified from celery cv. ‘Jinnanshiqin’. In celery, the expression of AgMYB5 exhibited transactivation activity, tissue specificity, and drought-condition responsiveness. Further analysis proved that ectopic expression of AgMYB5 increased β-carotene content and promoted drought tolerance in transgenic Arabidopsis thaliana. Moreover, AgMYB5 expression promoted β-carotene biosynthesis by triggering the expression of AtCRTISO and AtLCYB, which in turn increased antioxidant enzyme activities, and led to the decreased contents of H2O2 and MDA, and the inhibition of O2− generation. Meanwhile, β-carotene accumulation promoted endogenous ABA biosynthesis of transgenic Arabidopsis, which resulted in ABA-induced stomatal closing and delayed water loss. In addition, ectopic expression of AgMYB5 increased expression levels of AtERD1, AtP5CS1, AtRD22, and AtRD29. Conclusions The findings indicated that AgMYB5 up-regulated β-carotene biosynthesis and drought tolerance of Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

AgMYB5, an MYB transcription factor from celery, enhanced β-carotene synthesis and promoted drought tolerance in transgenic Arabidopsis

Sun

Zhu

et al. 2023

BMC Plant Biol

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“…ROS regulate the trade-off between growth and defence, where a central transcriptional network regulates the growth and defence pathways to balance the fate of the plant. Therefore, stresses can trigger oxidative stress, and thus, limit vegetative growth and reproduction capacity [21]. On the basis of MDA content during different stages of plant development, it is evident that the adverse effects are accompanied by structural alterations in the membrane [22] which were subsequently followed by cellular leakage of electrolytes and amino acids, diversion of electron flow toward alternate pathways [23], alterations in protoplasmic streaming and re-distribution of intracellular calcium ions.…”

Section: Discussionmentioning

confidence: 99%

Morphological Characterization and Germination-Based Screening for Cold Stress Response of Vigna radiata L.

Manasa

Panigrahy

Panigrahi

et al. 2022

IJPSS

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Aims: Morphological characterization and germination based screening for cold stress response of Vigna radiata, was performed to identify the cold resistant genotypes. Methodology: Augmented design experiment was performed to characterize 204 mungbean genotypes under field condition based on 24 DUS characterization. A germination-based screening at 10°C was performed in a total of 204 germplasms in a plant growth chamber to identify the cold responsive genotypes. Seeds were soaked in water for 6h in the dark under normal temperature and imposed cold stress (100C) for 2, 4, 6, 8 days and further transferred to control temperature (220C) for recovery. Results: On the basis of the 24 DUS characterizations of 204 mungbean genotypes are classified into three major clusters. Highest number of genotypes in cluster I (113 genotypes), followed by 81 genotypes in cluster II and least number (10) of genotypes in cluster III. Based on cold stress study and biplot analysis showed that germination percentage, germination rate index, Timson’s index, mean germination time and coefficient of velocity of germination to be more influential and categorized into three groups having 11 genotypes are resistant, 135 genotypes are susceptible, and 58 genotypes are intermediate. Biochemical analysis showed that the tolerant genotype having increased chlorophyll, carotenoid and MDA content and oxidative enzyme activity as compared with susceptible and intermediate genotypes under cold stress. Conclusion: The present study identified 11 genotypes having cold resistant and may be used for breeding program.

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“…These metabolites can significantly lower ROS and prevent cell damage, complementing the enzymatic antioxidant systems [41]. Together with photosynthetic pigments and osmolytes, they have been successfully used as drought stress biomarkers [10,18,42,43]. The build-up of osmolytes (or compatible solutes) in the cytosol is a typical response in plants to compensate for osmotic imbalance.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Drought Responses in Two Tropaeolum Species Used in Landscaping through Morphological and Biochemical Markers

Mircea

Calone²,

Shakya

et al. 2023

Life

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One of the most important challenges horticultural crops confront is drought, particularly in regions such as the Mediterranean basin, where water supplies are usually limited and will become even scarcer due to global warming. Therefore, the selection and diversification of stress-tolerant cultivars are becoming priorities of contemporary ornamental horticulture. This study explored the impact of water stress on two Tropaeolum species frequently used in landscaping. Young plants obtained by seed germination were exposed to moderate water stress (half the water used in the control treatments) and severe water stress (complete withholding of irrigation) for 30 days. Plant responses to these stress treatments were evaluated by determining several growth parameters and biochemical stress markers. The latter were analysed by spectrophotometric methods and, in some cases, by non-destructive measurements using an optical sensor. The statistical analysis of the results indicated that although the stress responses were similar in these two closely related species, T. minus performed better under control and intermediate water stress conditions but was more susceptible to severe water stress. On the other hand, T. majus had a stronger potential for adaptation to soil water scarcity, which may be associated with its reported expansion and naturalisation in different regions of the world. The variations in proline and malondialdehyde concentrations were the most reliable biochemical indicators of water stress effects. The present study also showed a close relationship between the patterns of variation of flavonoid and chlorophyll contents obtained by sensor-based and spectrophotometric methods.

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Validity of photo-oxidative stress markers and stress-related phytohormones as predictive proxies of mortality risk in the perennial herb Plantago lanceolata

Cited by 11 publications

References 59 publications

AgMYB5, an MYB transcription factor from celery, enhanced β-carotene synthesis and promoted drought tolerance in transgenic Arabidopsis

AgMYB5, an MYB transcription factor from celery, enhanced β-carotene synthesis and promoted drought tolerance in transgenic Arabidopsis

Morphological Characterization and Germination-Based Screening for Cold Stress Response of Vigna radiata L.

Evaluation of Drought Responses in Two Tropaeolum Species Used in Landscaping through Morphological and Biochemical Markers

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