Validity of aqueocentesis as a component of anterior uveitis investigation in dogs and cats

Linn-Pearl, Rose N.; Powell, Roger M.; Newman, Hayley A.; Gould, David

doi:10.1111/vop.12245

Cited by 24 publications

(36 citation statements)

References 10 publications

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“…It is specifically useful in aiding diagnosis of lymphoma . Most other intraocular tumors, either primary or secondary, do not seem to exfoliate into the aqueous humor with rare reports of mast cell tumors and large cell carcinomas . In the present case, cytologic examination of aqueous humor allowed a presumptive diagnosis of carcinoma.…”

Section: Discussionmentioning

confidence: 60%

“…Cytologic examination of aqueous humor has been reported to have low diagnostic utility, but it may be useful for diagnosing neoplastic uveitis . It is specifically useful in aiding diagnosis of lymphoma . Most other intraocular tumors, either primary or secondary, do not seem to exfoliate into the aqueous humor with rare reports of mast cell tumors and large cell carcinomas .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

What is your diagnosis? Aqueous humor from a dog

Ferreira¹,

Scurrell²,

Bass³

et al. 2019

Veterinary Clinical Pathol

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

What is your diagnosis? Aqueous humor from a dog

Ferreira¹,

Scurrell²,

Bass³

et al. 2019

Veterinary Clinical Pathol

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“…В ветеринарной офтальмологии, согласно литературным данным, показаниями для ППК являются: гифема, травма передней капсулы хрусталика с последующим увеитом, эндотелиальный отек роговицы, передний увеит, эндофтальмит, отек хориоидеи и геморрагии [7,14]. Также пункция ПК и взятие ВГЖ показаны в диагностических целях: для анализа цитологического материала, определения титра антител, постановки ПЦР и других лабораторных исследований [18,23].…”

Section: Discussionunclassified

Safety and efficacy of anterior chamber paracentesis with simultaneous intracameral administration of 0.5% pilocarpine in dogs and cats with glaucoma of various etiologies

Бояринов¹,

Boyarinov

2019

Russian Veterinary Journal

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Purpose. In animals with glaucoma to determine the technique for conducting ACP with simultaneous administration of a 0.5 % pilocarpine solution, to identify the dynamics of IOP reduction, as well as effectiveness of this procedure both after and in the long term. Based on analysis of data obtained, highlight the complications of this procedure. Materials and methods. Study included 37 animals (39 eyes): 24 dogs and 13 cats with glaucoma, which, according to anamnesis, developed no more than 10 days without any preliminary ocular hypotensive therapy. All animals underwent ACP without general anesthesia or sedation. Immediately before procedure, animals were instilled with a local anesthetic in conjunctival sac (0.5% proxymethacaine). ACP was performed under 6-fold magnification using a 27G headlamp with 9 hours for right eye and 3 hours for left eye. A puncture was carried out at periphery of cornea 1 mm from limbus. Volume of aspirated AH was no more than 2 drops. After that, needle was removed from the AC. Next step was intracameral injection of 0.5% solution of pilocarpine through hole of paracentesis. Volume of injected solution did not exceed 0.3 ml. After paracentesis and administration of pilocarpine solution, subconjunctival administration of dexamethasone solution in combination with gentamicin and novocaine was performed. Immediately after ACP, antihypertensive therapy was prescribed from a combination of eye drops: synthetic analogues of prostaglandins F2α (travoprost 0.004%) in combination with non-selective beta-blockers (timolol maleate 0.5%), as well as carbonic anhydrase inhibitors (brinzolamide 1%) every 12 hours. Results. Average IOP was in animals before ACP 60.26 ± 16.32 mmHg; immediately after procedure ― 10.15 ± 3.23 mm Hg; 1 hour after ACP ― 13.28 ± 3.09 mm Hg; after 7 days ― 17.61 ± 6.03. Immediately after intracameral administration of a 0.5% pilocarpine solution, we observed a rapid and pronounced narrowing of the pupil within 1…2 minutes. Myosis was noted for at least 1 hour after procedure. There were no adverse reactions from administration of pilocarpine solution in AC. Among complications after immediately 1 hour after the ACP, we encountered aqueous flare in 12 eyes (31% of cases) and moderate hyphema in 5 eyes (13% of cases). 7 days after procedure, no complications were observed. Conclusion. This study shows that ACP with simultaneous intracameral administration of 0.5% pilocarpine solution is effective in dogs and cats without any significant side effects. These results allow us to offer ACP as a procedure for an emergency reduction in IOP, especially in acute attacks of glaucoma in dogs and cats.

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“…As aqueous humor was collected postmortem in the present study, a 22 ‐ G needle was used for paracentesis to increase the amount of sample material and facilitate the collection. If performed antemortem, 27‐ to 29‐G needles are usually used to reduce the risk of ocular damage . The use of the larger needle could have resulted in improved cell retrieval and reduced cell disruption which might be a problem in antemortem samples.…”

Section: Discussionmentioning

confidence: 99%

“…Aqueous humor is commonly obtained in cats with uveitis for cytology but is often nondiagnostic in cats with infectious uveitis . A mixed inflammatory cell population including macrophages is usually present within the anterior eye segment of cats suffering from uveitis secondary to FIP …”

Section: Introductionmentioning

confidence: 99%

Utility of an immunocytochemical assay using aqueous humor in the diagnosis of feline infectious peritonitis

Felten

Matiasek²,

Gruendl

et al. 2017

Veterinary Ophthalmology

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Objective In cats suffering from feline infectious peritonitis (FIP) without effusion, antemortem diagnosis is challenging. Uveitis is common in these cats. It was the aim of this study to evaluate sensitivity and specificity of an immunocytochemical assay (ICC) in aqueous humor of cats suspected of having FIP. Animals studied The study included 26 cats with immunohistochemically confirmed FIP and 12 control cats for which FIP was suspected due to similar clinical or laboratory changes, but which suffered from other diseases confirmed via histopathology. Procedures All aqueous humor samples were collected postmortem by paracentesis. ICC was carried out as avidin-biotin complex method. Sensitivity, specificity, and the overall accuracy including 95% confidence intervals (95% CI) were calculated. Results Immunocytochemistry was positive in 16 of 25 cats with FIP and 2 of 11 control cats (one cat with lymphoma, one with pulmonary adenocarcinoma). Aqueous humor samples of one cat with FIP and of one control cat were excluded from statistical analysis. Sensitivity was 64.0% (95% CI: 42.5-82.0); specificity 81.8% (95% CI: 48.2-97.7); and overall accuracy 69.4% (95% CI: 51.9-83.7). Conclusions As false-positive results occurred and specificity is most important in the diagnosis of FIP, the diagnostic utility of ICC in aqueous humor is limited. Further studies are required to clarify the origin of false-positive ICC results.

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Validity of aqueocentesis as a component of anterior uveitis investigation in dogs and cats

Abstract: Aqueocentesis is performed readily with minimal risk. The results were primarily useful in aiding a diagnosis of lymphoma in both dogs and cats.

Cited by 24 publications

References 10 publications

What is your diagnosis? Aqueous humor from a dog

What is your diagnosis? Aqueous humor from a dog

Safety and efficacy of anterior chamber paracentesis with simultaneous intracameral administration of 0.5% pilocarpine in dogs and cats with glaucoma of various etiologies

Utility of an immunocytochemical assay using aqueous humor in the diagnosis of feline infectious peritonitis

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