Validation Study of the “Onestep ABAcard® PSA Test” Kit for RCMP Casework

Kearsey, J.; Louie, H.; Poon, Hiron

doi:10.1080/00085030.2001.10757518

Cited by 19 publications

(15 citation statements)

References 18 publications

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“…PSA is detectable in vaginal discharge for up to 48 hours after acts of unprotected vaginal intercourse and therefore, can be used as a biological indicator of unprotected sex (5,20,21). Before interviewing consenting participants, trained lab technicians collected vaginal secretions and administered rapid PSA tests at the interview sites.…”

Section: Methodsmentioning

confidence: 99%

“…Before interviewing consenting participants, trained lab technicians collected vaginal secretions and administered rapid PSA tests at the interview sites. Presence of PSA was tested using the rapid ABAcard p30 test (Abacus Diagnostics, West Hill, CA), which has been shown to be reliable, sensitive, specific (20,21) and comparable to the quantitative PSA assay(5). Extraction of specimens from fresh vaginal swabs was performed in the extraction buffer provided with the testing kit and swabs were soaked for at least 5 minutes.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Biomarker validation of self-reported sex among middle-aged female sex workers in China

Guida

Fukunaga

Liu

2017

Annals of Epidemiology

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Biomarker validation of self-reported sex among middle-aged female sex workers in China

Guida

Fukunaga

Liu

2017

Annals of Epidemiology

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“…Objective markers of semen exposure, such as prostate-specific antigen (PSA), also have been used for this purpose. Long used in the forensic sciences for sexual assault cases, PSA is found in high concentrations in semen and serves as a reliable, valid biomarker for recent (<48 h) semen exposure [13][14][15][16]. In controlled trials, the sensitivity of PSA tests to the presence of even moderate levels of semen in the vagina was 99 % [17].…”

Section: Introductionmentioning

confidence: 97%

Exploring Discordance Between Biologic and Self-Reported Measures of Semen Exposure: A Qualitative Study Among Female Patients Attending an STI Clinic in Jamaica

Carter¹,

Bailey

Snead³

et al. 2012

AIDS Behav

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Correspondence to: Marion W. Carter, acq0@cdc.gov. HHS Public Access Author Manuscript Author ManuscriptAuthor Manuscript Author ManuscriptWe explored the use of qualitative interviews to discuss discrepancies between two sources of information on unprotected sex: biomarker results and self-reported survey data. The study context was a randomized trial in Kingston, Jamaica examining the effect of STI counseling messages on recent sexual behavior using prostate-specific antigen (PSA) as the primary study outcome. Twenty women were interviewed. Eleven participants were selected because they tested positive for PSA indicating recent semen exposure, yet reported no unprotected sex in a quantitative survey ("discordant"): 5 reported abstinence and 6 reported condom use. Nine participants who also tested positive for PSA but reported unprotected sex in the survey were interviewed for comparison ("concordant"). Qualitative interviews with 6 of the 11 discordant participants provided possible explanations for their PSA test results, and 5 of those were prompted by direct discussion of those results. Rapid PSA testing combined with qualitative interviews provides a novel tool for investigating and complementing self-reported sexual behavior.

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“…Commercial test kits that depend on antibody-antigen reaction are currently widely used for PSA detection. One of the most popular commercial kits, the OneStep ABAcard® PSA, also uses the technique of a mobile monoclonal anti-human PSA antibody which binds to human PSA and migrates along the strip, forming a visible line in its path (33,34). However, studies have shown that PSA can also be found in very low levels in other body fluids, such as female urine and breast milk (35,36).…”

Section: Immunological Testsmentioning

confidence: 99%