Validation rules for blood smear revision after automated hematological testing using Mindray CAL-8000

Abstract

These results show that customization of validation rules is necessary for enhancing the quality of hematological testing and optimizing workflow.

Comparing the performance of three panels rules of blood smear review criteria on an Italian multicenter evaluation

“…In the present study, the WGH and IS rules generated a high percentage of OM reviews (ie, 34.9% and 36.7%, respectively). Nonetheless, their performed better than 41‐ICGH rules (39%), which is confirmed by data reported in the international literature …”

“…Similarly, the percentage of FP samples given through the application of WGH rules (ie, 11.7%) was lower than by 41‐ICGH and IS rules (ie, 15.1% and 13.7%). The comparison of our data with those previously published in literature shows that the percentage of FP and FN samples is comparable or even better than what has been obtained earlier, wherein these percentages ranged between 11.3% and 17.3% for FP samples and between 2.2% and 4.3% for FN samples . Notably, as for the different panels rules adopted from time to time on each HAs, if we analyze their performance singularly, there is a clear evidence of a different behavior in terms of SE and SP, as detailed in Table .…”

“…According to the assumptions made above, the best option to take first into consideration to reach this goal is the adoption of a blood smear review criteria customized and calibrated on the specific type of HA technology used by the hematology laboratory. This simple action proved to be effective in many cases, as evidenced by current literature …”

“…ICGH guidelines have been introduced in 2005, and many studies have been conducted to determine their performance and the following updates, literature data have often showed a great variability of results on ICGH rules performances when applied on different HAs, even on those based on similar technology . All these observations confirm that any panel rules of procedure on blood smear review criteria should be carefully evaluated and customized on the basis of the technology used in each laboratory and its particular “case mix” …”

“…The current literature offers a wide variety of suggestions and useful panel rules of procedure on blood smear review criteria following CBC analysis . ICGH guidelines have been introduced in 2005, and many studies have been conducted to determine their performance and the following updates, literature data have often showed a great variability of results on ICGH rules performances when applied on different HAs, even on those based on similar technology .…”