Validation of two major quantitative trait loci for fusarium head blight resistance in Chinese wheat line W14

Chen, J.; Griffey, Carl A.; Maroof, M. A. Saghai; Stromberg, Erik L.; Biyashev, R. M.; Zhao, Wenhui; Chappell, Matthew; Pridgen, Thomas H.; Dong, Yanhong; Zeng, Zhao‐Bang

doi:10.1111/j.1439-0523.2006.01182.x

Cited by 86 publications

(81 citation statements)

References 18 publications

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“…For example, QTLs conditioning low DON content on chromosomes 3BS and 5AS Chen et al 2006;Ma et al 2006) had common intervals with QFdk.nau-3B and QFdk.nau-5A. This is probably why FDK is correlated with DON content (Bai et al 2001;Paul et al 2005).…”

Section: Mesterhazymentioning

confidence: 93%

Mapping QTLs associated with Fusarium-damaged kernels in the Nanda 2419 × Wangshuibai population

Zhu

Zhang

et al. 2007

Euphytica

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Fusarium head blight (FHB), or scab, is a devastating wheat disease worldwide, reducing both grain yield and quality. The percentage of Fusariumdamaged kernels (FDK) directly reflects the damage level caused by scab on wheat grains and its variation represents the so-called type IV scab resistance in germplasm. To identify genes governing type IV resistance and investigate its relationship with other scab resistance types, we mapped QTLs associated with percent FDK using data from three different field evaluations of the recombination inbred line (RIL) population derived from the susceptible cultivar Nanda 2419 9 the scab-resistant cultivar Wangshuibai. Five QTLs related to percent FDK were identified in at least two different trials, for which Wangshuibai contributed four of the resistance alleles. Most of the FDK-related QTLs, including the three with larger effects, QFdk.nau-2B, QFdk.nau-3B and QFdk.nau-4B, mapped to intervals associated with either type IV resistance or type II resistance. Moreover, most of the major type I and type II resistance QTLs detected previously were associated with type IV resistance, suggesting that resistance to initial infection and disease spread play major roles in conditioning less FDK. Therefore, breeders have options to choose inoculation methods based on their expertise and resources without risking significant loss of information when using percent FDK as the disease index. The most useful scab resistance QTLs for breeding would be those with stable influences on FDK and/or deoxynivalenol (DON) accumulation besides the initial infection and disease spread.

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Section: Mesterhazymentioning

confidence: 93%

Mapping QTLs associated with Fusarium-damaged kernels in the Nanda 2419 × Wangshuibai population

Zhu

Zhang

et al. 2007

Euphytica

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“…At present, several genes related to DON resistance have been isolated from various plants, such as acetyltransferase in wheat [9] , the ribosomal protein L3 (RPL3) gene in rice [10] and wheat [11] , the DOGT1 gene of the UDP-glycosyltransferases (UGTs) family in A. thaliana [12] , and the PDR type ATP-binding cassette (ABC) transporter in Saccharomyces cerevisiae [12,13] . In wheat, FHB1, a major QTL of FHB resistance and DON resistance in chromosome arm 3BS has been identified [14][15][16][17] , and it probably encodes or regulates DON detoxification proteins [18] . In order to better understand molecular mechanisms of FHB or DON resistance, it is critical to identify new genes that are expressed closely upon DON treatment, because the inheritance of FHB resistance and DON resistance is a complicated quantitative trait and mechanisms under FHB resistance have not yet been clarified.…”

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confidence: 99%

Characterization of a PDR type ABC transporter gene from wheat (Triticum aestivum L.)

Shang

Xiao

et al. 2009

Chin. Sci. Bull.

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DON, as a virulence factor, plays an important role in the infection of Fusarium graminearum in wheat.The infection ability of F. graminearum depends on its capacity of producing DON. The production of DON by F. graminearum is significantly decreased in the wheat varieties with scab resistance. In this study, GeneChip analysis indicated that an EST encoding an ATP-binding cassette (ABC) transporter was up-regulated by 45 times in a wheat landrace Wangshuibai, which is resistant to DON accumulation. A pair of EST-derived primers were designed based on the EST sequence, and a clone was then isolated from a wheat genomic DNA TAC library. The TAC clone was sequenced using chromosome walking and gene prediction was conducted using Softberry. A cDNA clone of this gene was subsequently isolated from Wangshuibai induced by DON using gene-specific primers designed according to the untranslated sequence of the gene. The genome size of the gene is 7377 bp, consisting of 19 exons with coding sequences of 4308 bp. It encodes a protein with 1435 amino acid residues and the calculated molecular weight is about 161 kD. BLAST analysis indicated that the gene may belong to pleiotropic drug resistance (PDR) sub-family, and hence designated as TaPDR1 (Triticum aestivum pleiotropic drug resistance). TaPDR1 was located on chromosome 5A of wheat using nullisomic-tetrasomic lines of Chinese Spring. TaPDR1 was up-regulated by induction of both DON and F. graminearum. Expression patterns of TaPDR1 were different in wild-type Wangshuibai and the fast-neutron induced Wangshuibai mutant lacking FHB1, a major QTL of FHB resistance and DON resistance in chromosome arm 3BS. These results suggested that TaPDR1 might be a candidate gene responsible for DON accumulation resistance. The expression profile showed that TaPDR1 expression was neither induced by hormones typically involved in biotic stress, such as JA and SA, nor by abiotic stresses, such as heat, cold, wounding and NaCl. However, TaPDR1 expression was regulated by Al 3+ and [Ca 2+ ], indicating that [Ca 2+ ] i might mediate the signal of TaPDR1 expression. wheat, deoxynivalenol (DON), ABC transporter, PDR (pleiotropic drug resistance)Fusarium head blight (FHB) is a fungal disease caused by Fusarium graminearum, which can infect wheat, barley, small grain cereals and maize, and has become a destructive disease worldwide. FHB causes an enormous yield loss [1] . More seriously, it deteriorates grain quality by contaminating infected grains with trichothecene toxins, thus bringing food safety problems. Deoxynivalenol (DON) is one of the most important toxin members [2] . It inhibits syntheses of DNA, RNA and protein in eukaryotic cells, affects mitochondrial functions, suppresses cell division and changes plasma membrane permeability. In addition, DON can act as a virulence factor and plays an important role in FHB-induced plant diseases [3] . There are two stages in the process of the infection of FHB by Fusarium graminearum, including biotrophy and necrotrophy. In the initial stage of in...

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“…Several quantitative trait loci (QTLs) are associated with type I resistance in recombinant inbred lines (RILs) and doubled haploid lines (DHLs) (Chen et al 2006, Gilsinger et al 2005, Lin et al 2006, Steed et al 2005, Steiner et al 2004, Yang et al 2005, Yu et al 2008; reviewed by Buerstmayr et al 2009, Liu et al 2009. Since this type of resistance is more difficult to assess, less is known about it than other types (Buerstmayr et al 2009); for example, the mechanism of type I resistance is not clear (Wagacha and Muthomi 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of cleistogamy on Fusarium head blight resistance in wheat

et al. 2010

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In wheat (Triticum aestivum L.), Fusarium head blight (FHB) resistance is an important breeding target to reduce yield losses and mycotoxin contamination. We hypothesized that cleistogamous (CL, closed flowering) cultivars have a lower risk of FHB infection than chasmogamous (CH, opened flowering) cultivars because FHB infection usually occurs in the inside of florets or extracted anthers and FHB resistance in CL cultivars decreases mycotoxin accumulation. To test these hypotheses, we produced 126 recombinant inbred lines (RILs) derived from a cross between common wheat varieties 'U24' (CL variety) and 'Saikai 165' (CH variety). Although 'U24' and 'Saikai 165' are both resistant to FHB infection, 'U24' is susceptible to FHB spread, grain deterioration, and mycotoxin accumulation compared with 'Saikai 165'. Among the RILs, there were significant differences in all of the tested traits. Although the CL RILs showed less initial FHB infection than CH RILs, there were no significant differences in grain deterioration and mycotoxin accumulation between the 2 groups. An FHB-resistant quantitative trait locus (QTL) located on chromosome 3BS increased resistance to FHB spread, grain deterioration, and mycotoxin accumulation; however, not all CL RILs with this QTL accumulated less mycotoxin than 'Saikai 165'.

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Validation of two major quantitative trait loci for fusarium head blight resistance in Chinese wheat line W14

Cited by 86 publications

References 18 publications

Mapping QTLs associated with Fusarium-damaged kernels in the Nanda 2419 × Wangshuibai population

Mapping QTLs associated with Fusarium-damaged kernels in the Nanda 2419 × Wangshuibai population

Characterization of a PDR type ABC transporter gene from wheat (Triticum aestivum L.)

Effect of cleistogamy on Fusarium head blight resistance in wheat

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