Validation of the Osteomyelitis Induced by Methicillin-Resistant Staphylococcus aureus (MRSA) on Rat Model with Calvaria Defect

Choi, Young Suk; Ham, Dae Sung; Lim, Ji Yun; Lee, Young Koo

doi:10.1007/s13770-021-00340-5

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…However, Choi et al showed that MRSA had distinct effects on bone cells (in vitro or in vivo). Detecting significant necrosis rates in rats, they suggested that bone necrosis was dose dependent (31). According to our AO/EB dual staining results, cell death increased significantly in the presence of MRSA, MRSA+HNE and MRSA+LNE and this cell death occurred more with the necrosis According to our ROS detection results, there was no significant increase in ROS production in the presence of NE and/or MRSA which confirms that cell death does not occur via the apoptosis pathway.…”

Section: Discussionsupporting

confidence: 50%

Cross-interactions between Norepinephrine, Methicillin-Resistant Staphylococcus aureus and Human Osteoblast Cells in Culture Conditions

Yüksek¹,

Gümüş²,

Gündoğan³

et al. 2022

experimed

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Objective:The role of norepinephrine (NE) on growth, adhesion and invasion of methicillin-resistant Staphylococcus aureus (MRSA) ATTC 43300 was examined in human osteoblast (HOB) cells. The effects of NE and/or MRSA on the viability and cell death pathways of HOB cells were also investigated. Furthermore, the alterations of bacterial response to oxidative stress (H 2 O 2 ) were analyzed in the presence/ absence of NE.Materials and Methods: Bacterial growth was detected spectrophotometrically. The colony counting method was examined for adhesioninvasion. The alteration of HOB cell viability was determined by methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay. The death pathways of HOB cells were examined microscopically using acridine orange-ethidium bromide dual staining and dichlorofluoresceindiacetate (DCF-DA) dye. The bacterial response to H 2 O 2 was investigated by agar dilution. Results:The growth of bacterium was not affected in the presence of NE. Bacterial adhesion was decreased by NE (p<0.0001) while highlevel NE induced invasion (p=0.013). HOB cell viability was reduced by MRSA and/or NE (p<0.001). MRSA and co-existence of MRSA and NE caused necrosis more than apoptosis in HOB cells (p<0.05). NE did not alter the bacterial response to oxidative stress. Conclusion:Norepinephrine has different effects on the biological properties of both MRSA and HOB cells.

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Section: Discussionsupporting

confidence: 50%

Cross-interactions between Norepinephrine, Methicillin-Resistant Staphylococcus aureus and Human Osteoblast Cells in Culture Conditions

Yüksek¹,

Gümüş²,

Gündoğan³

et al. 2022

experimed

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“…The sampled bacteria also displayed sufficient susceptibility to moxifloxacin but poor susceptibility to clindamycin, with only 50% of bacteria being susceptible [30]. Surgery, performed by an otolaryngologist, can remove excess tissue or bone from the sinus cavity to improve drainage and lower the risk of sinusitis [43]. Previously, the Caldwell-Luc method was used to treat chronic sinusitis, but postoperative complications such as buccal skin discomfort and sinusitis recurrence were common following this procedure [44].…”

Section: Discussionmentioning

confidence: 99%

“…The otolaryngologist may also prescribe antibiotics to treat the infection and stop it from spreading [42]. The microbiome of purulent odontogenic maxillary sinusitis showed anaerobic dominance, and collected bacteria exhibited sufficient susceptibility rates to ampicillin/sulbactam (80%) and piperacillin/tazobactam (93.3%) [30,43]. The sampled bacteria also displayed sufficient susceptibility to moxifloxacin but poor susceptibility to clindamycin, with only 50% of bacteria being susceptible [30].…”

Section: Discussionmentioning

confidence: 99%

Change in Maxillary Sinus Mucosal Thickness in Patients with Preoperative Maxillary Sinus Mucosal Thickening as Assessed by Otolaryngologists: A Retrospective Study

Kim,

Min,

et al. 2023

Medicina

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Background and Objectives: Maxillary sinus pathologic conditions may increase the risk of complications during posterior maxillary sinus augmentation surgery. The purpose of this study was to evaluate the changes in participants with preoperative maxillary sinus mucosal thickening and to assess this factor as a preoperative risk indicator for sinusitis after maxillary dental implantation. Materials and Methods: We compared the preoperative and postoperative maxillary sinus mucosal thickness (MSMT), the distance between the maxillary sinus ostium and sinus floor (MOD), and the MSMT/MOD ratio. The participants were divided into three groups (sinus augmentation, bone grafting, and no grafting). Results: The mean preoperative MSMT was 4.3 ± 2.0 mm, and the mean MSMT/MOD ratio was 0.13 ± 0.05. No postoperative sinusitis was observed in these patients, including cases caused by anatomical variations. The mean postoperative MSMT was 4.5 ± 2.3 mm, and the mean postoperative MSMT/MOD ratio was 0.15 ± 0.06. There was no statistically significant difference between the groups at each time point (p > 0.05). Conclusions: The study found no significant change in MSMT at post-treatment evaluation, even when considering different subgroups. It underscores the importance of preoperative maxillary sinus radiographic assessments and collaboration between dentists and otolaryngologists for better outcomes in patients with preoperative maxillary sinus mucosal thickening.

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“…Rat models have also been utilized for the study of osteomyelitis in the calvarial [ 60 , [61] , [62] , [63] , [64] ] and maxillofacial [ 65 , 66 ] region. Critical-sized defects with a diameter of 5–8 mm were created on the middle of the parietal bone or bilateral, with simultaneous evaluation of bone repair and anti-bacterial status [ 64 ].…”

Section: Animal Models For Post-traumatic Osteomyelitis (Pto)mentioning

confidence: 99%

“…Inoculation was performed through direct injection of up to 6 × 10 5 CFU/5 μL S. aureus subsp. aureus (ATCC 43300) [ 61 ], 0.1 mL of bacteria suspension containing 10 5 –10 8 CFU of S. aureus suspended in tryptic soy broth (TSB) medium or phosphate-buffered saline (PBS) [ 63 , 64 ], or resorbable collagen sponge soaked with 10 7 CFU of MSSA [ 62 ]. The observation period generally lasted for 4 weeks, with debridement performed 7 days postoperatively [ 62 ].…”

Section: Animal Models For Post-traumatic Osteomyelitis (Pto)mentioning

confidence: 99%

Choosing the right animal model for osteomyelitis research: Considerations and challenges

Huang,

Wen,

Zhang

et al. 2023

Journal of Orthopaedic Translation

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Validation of the Osteomyelitis Induced by Methicillin-Resistant Staphylococcus aureus (MRSA) on Rat Model with Calvaria Defect

Cited by 7 publications

References 38 publications

Cross-interactions between Norepinephrine, Methicillin-Resistant Staphylococcus aureus and Human Osteoblast Cells in Culture Conditions

Cross-interactions between Norepinephrine, Methicillin-Resistant Staphylococcus aureus and Human Osteoblast Cells in Culture Conditions

Change in Maxillary Sinus Mucosal Thickness in Patients with Preoperative Maxillary Sinus Mucosal Thickening as Assessed by Otolaryngologists: A Retrospective Study

Choosing the right animal model for osteomyelitis research: Considerations and challenges

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