Validation of the Microreader™ 23sp ID system: A new STR 23-plex system for forensic application

Li, Jienan; Luo, Haibo; Song, Feng; Zhang, Lushun; Deng, Chuncao; Yu, Zailiang; Gao, Tianxi; Liao, Miao; Hou, Yiping

doi:10.1016/j.fsigen.2016.12.005

Cited by 24 publications

(12 citation statements)

References 12 publications

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“…To verify whether new STRs are suitable for forensic application, it is necessary to perform their detailed genomic characterization and conduct a number of population surveys (8). Detailed studies of the gene sequence information of these 22 loci, especially the newly adopted non-CODIS STRs, as well as the validation studies on the sensitivity, accuracy, and species specificity of this new panel have been performed (1,8). Polymorphisms of these loci in Han, Li, Hui, and Uygur populations in some regions of China have also been reported (2-7).…”

Section: Discussionmentioning

confidence: 99%

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Multiple genetic analyses to investigate the polymorphisms of Chinese Mongolian population with an efficient short tandem repeat panel

et al. 2019

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Aim To determine allele frequencies and forensic statistics of 22 autosomal short tandem repeat loci in Chinese Mongolian population. Methods Blood specimens were collected from 134 unrelated healthy Mongolian individuals, and 22 short tandem repeat loci were co-amplified and genotyped. Allele frequencies and forensic parameters were calculated, and population genetic differences were analyzed among Mongolian population and other eight Chinese populations: Northern Han, Guangdong Han, Chengdu Han, Xinjiang Hui, Xinjiang Uygur, Hainan Li, Qinghai Tibetan, and Hainan Han. Results All the loci were in the Hardy-Weinberg equilibrium, and after Bonferroni correction there was no linkage disequilibrium between them. The allele frequencies of these 22 loci were between 0.0037 and 0.3657. This panel had high discriminating power and genetic polymorphism in the Mongolian population, with combined power of discrimination of 0.999999999999999999999999998399 and combined probability of exclusion of 0.9999999999566925. Structure analysis showed no evidence that these nine Chinese populations had different component distribution. However, genetic distance analysis showed significant differences among them ( P < 0.05). Conclusion The combined application of these 22 loci could be useful for forensic purposes in the Mongolian population. Mongolian population had smaller genetic distances from the populations in northern China (Northern Han, Xinjiang Uygur, and Xinjiang Hui) than from the populations in Hainan province (Hainan Han and Hainan Li populations).

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Section: Discussionmentioning

confidence: 99%

“…This panel was validated by a previous study (1), which assessed its sensitivity, accuracy, precision, stability, stutter percentage, peak height ratio, and species specificity. It was used to analyze allelic distribution in Northern Han (2), Southern Han (3), Chengdu Han (4), Hainan Li (5), Xinjiang Hui (6), and Xinjiang Uygur (7).…”

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confidence: 99%

Multiple genetic analyses to investigate the polymorphisms of Chinese Mongolian population with an efficient short tandem repeat panel

et al. 2019

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“…PCR amplification was implemented on the GeneAmp ® PCR 9700 thermocycler (Applied Biosystems, Foster City, CA, USA) with 25 µL reaction volume, and amplified products were subsequently separated and analyzed on the ABI PRISM ® 3130XL Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) with reference to internal lane standard Org500 (including different length fragments: 50, 75, 100, 139, 150, 160, 200, 300, 340, 350, 400, 450, 490 and 500 bp). The specific reaction system and conditions for electrophoresis were referred to the previously published research (Li et al, 2017). The analyzation of electrophoresis results were revealed by GeneMapper ® ID-X 1.3 software (Applied Biosystems, Foster City, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…However, in forensic applications, it is indispensable to combine the non-CODIS STRs with the previously commercial CODIS STR kits in order to increase the discrimination power (Inokuchi et al, 2018; Kuzniar, Jastrzebska & Ploski, 2006; Tsai et al, 2013) in some cases, like missing person investigations, complicated parentage testing cases and those with mutation events. In this study, 22 selected loci (Li et al, 2017) were introduced containing 18 non-CODIS STR loci and four CODIS STR loci in which three (D1S1656, D2S1338 and D12S391) were recognized as CODIS core loci in 2015 (Hares, 2015) and one (D16S539) belonged to 13 CODIS core loci.…”

Section: Introductionmentioning

confidence: 99%

Forensic characteristics and population genetics of Chinese Kazakh ethnic minority with an efficient STR panel

Chen

Guo

Jin

et al. 2019

PeerJ

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On the purpose of enhancing the forensic efficiency of CODIS STR loci, new STR loci have been gradually discovered and developed into some commercial multiplex systems. Recently, 22 STR loci including 18 non-CODIS STR loci and four CODIS STR loci were investigated in 501 unrelated healthy individuals of Kazakh ethnic group. Seven to 20 alleles at the different loci were identified and altogether 276 alleles for 22 selected loci were detected with the corresponding allelic frequencies ranging from 0.0010 to 0.3623. No significant deviation was observed from the Hardy–Weinberg equilibrium test for any of the 22 STRs. The value of cumulative power of discrimination in Kazakh group was 1-1.00E−28. Analyses of population differentiations and genetic distances between Kazakh and other Chinese groups presented that the Kazakh group with the Uygur group. These 22 STR loci evenly distributed on 22 different autosomal chromosomes were characterized by high genetic diversities and therefore could be utilized in the forensic cases to further increase the discrimination performance.

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“…A set of 25 supplementary STRs was suggested by the National Institute of Standards and Technology (NIST) to increase the certainty in kinship testing 8 ; however, no multiplex combing these STRs is commercially available. Supplementary Kits: Microreader 23sp ID 9 (Suzhou Microread Genetics, China), Goldeneye DNA ID 22NC 10 (Goldeneye Technology Ltd., China), AGCU 21 + 1 11 (AGCU ScienTech Incorporation, China) have been developed, but they are only commercially available in China 12 .…”

Section: Introductionmentioning

confidence: 99%

An evaluation of the SureID 23comp Human Identification Kit for kinship testing

Alsafiah

Aljanabi

Hadi

et al. 2019

Sci Rep

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Short tandem repeat (STR) profiling has been routinely used in kinship testing since the introduction of commercial kits in the mid-1990s. While 15 to 23 STR loci normally give definitive results in simple kinship testing, additional loci are sometimes required to resolve complex cases. The SureID 23comp Human Identification Kit, recently released by Health Gene Technologies (China), multiplexes amelogenin and 22 autosomal STRs, 17 of which are non-CODIS STRs. This enables the profiling of 38–40 loci when used in conjunction with widely used commercial kits. In this study, the kit was evaluated for kinship applications as a supplementary STR kit following the minimum criteria for validation recommended by the European Network of Forensic Science Institutes (ENFSI) and the Scientific Working Group on DNA Analysis Methods (SWGDAM) using 500 samples. Performance was comparable with other commercial kits demonstrating: repeatability and reproducibility; precision (maximum s.d. 0.1048 nt); accuracy, all alleles were within ±0.41 nt compared to the actual sizes; heterozygous peak balances at all loci >68%; stutter ratios ranged from 3.8% to 16.15%; full profiles were generated with 125 pg DNA (95.12% of alleles at 62 pg),; and we found 100% concordance over 5 common STRs with the GlobalFiler kit.

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Validation of the Microreader™ 23sp ID system: A new STR 23-plex system for forensic application

Cited by 24 publications

References 12 publications

Multiple genetic analyses to investigate the polymorphisms of Chinese Mongolian population with an efficient short tandem repeat panel

Multiple genetic analyses to investigate the polymorphisms of Chinese Mongolian population with an efficient short tandem repeat panel

Forensic characteristics and population genetics of Chinese Kazakh ethnic minority with an efficient STR panel

An evaluation of the SureID 23comp Human Identification Kit for kinship testing

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