Validation of the Fluorescence Polarization Assay and Comparison to Other Serological Assays for the Detection of Serum Antibodies to Brucella Abortus in Bison

Gall, D.; Nielsen, Klaus; Forbes, Lorry B.; Davis, Donald S.; Elzer, Philip H.; Olsen, Steven C.; Balsevicius, S.; Kelly, L.; Smith, Phillip D.; Tan, S.; Joly, Damien O.

doi:10.7589/0090-3558-36.3.469

Cited by 34 publications

(23 citation statements)

References 11 publications

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“…We have recently described the use of FP assays, using a fluorescein-labeled OPS from Brucella abortus, for the detection of antibodies to Brucella spp. (a gram negative bacterium) in bovids, bison, cervids and swine [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. We have shown that this approach, in principle, can be applied to all gram negative bacteria, which possess OPSs, by extending it to the detection of antibodies to Salmonella spp.…”

Section: Introductionmentioning

confidence: 88%

“…The assay was evaluated against a variety of commonly used assays (the buffered plate antigen test, the rose bengal plate test, the complement fixation test, the rivanol agglutination test, competitive ELISA, indirect ELISA and culture) in bovine sera [6,[10][11][12][19][20], porcine sera [13][14], bison sera [15], cervid sera [16], bovine whole blood [8,17,19], and bovine milk [8,18,19,21]. In all these studies the FPA was equal, and many times superior to the commonly used assays in terms of sensitivity and specificity, often achieving 99+% for both.…”

Section: The Fpa For the Detection Of Brucella Spp In Animal Body Flmentioning

confidence: 99%

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The Use of Fluorescence Polarization Assays for the Detection of Infectious Diseases

Jolley¹,

Nasir²

2003

CCHTS

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Fluorescence Polarization Assays (FPAs) have been shown to have great utility in the detection of infectious diseases. Examples are presented of the use of O-polysaccharides (OPSs) for the detection of antibodies in serum, whole milk and whole blood to gram negative organisms (Brucella spp., Salmonella spp.). The use of proteins and peptides are also described for the detection of Mycobacterium bovis and Equine Infectious Anemia Virus. Fluorescence Polarization Inhibition Assays (FPIAs) are discussed for the specific and sensitive detection and quantitation of Salmonella spp. cells from culture. An example of the detection of enterohemorrhagic E. coli (EHECS) by Strand Displacement Amplification (SDA), coupled with FP, down to the single cell level, within thirty minutes, is described.

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Section: Introductionmentioning

confidence: 88%

Section: The Fpa For the Detection Of Brucella Spp In Animal Body Flmentioning

confidence: 99%

The Use of Fluorescence Polarization Assays for the Detection of Infectious Diseases

Jolley¹,

Nasir²

2003

CCHTS

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“…petitive enzyme-linked immunoabsorbent assay, standard plate, and standard tube tests (Huber and Nicoletti 1986, Gall et al 2000, Nielsen and Gall 2001, Nielsen 2002, Philo and Edwards 2002. After 2000, serology status was determined by veterinarians or trained National Park Service biologists using the standard card or fluorescence polarization assay (Gall et al 2000, Nielsen andGall 2001).…”

Section: Seroprevalencementioning

confidence: 99%

“…We modeled uncertainty in data on exposure to brucellosis (section Seroprevalence) using a meta-analysis of studies of serological test sensitivity and specificity (Gall et al 2000, Nielsen and Gall 2001, Gall and Nielsen 2004. We estimated the parameters for the distribution of the probability of a true positive and the probability of a false positive using published values, aggregating results across tests (Table 3).…”

Section: Parameter Modelsmentioning

confidence: 99%

State‐space modeling to support management of brucellosis in the Yellowstone bison population

Hobbs

Geremia

Treanor

et al. 2015

Ecological Monographs

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Abstract. The bison (Bison bison) of the Yellowstone ecosystem, USA, exemplify the difficulty of conserving large mammals that migrate across the boundaries of conservation areas. Bison are infected with brucellosis (Brucella abortus) and their seasonal movements can expose livestock to infection. Yellowstone National Park has embarked on a program of adaptive management of bison, which requires a model that assimilates data to support management decisions. We constructed a Bayesian state-space model to reveal the influence of brucellosis on the Yellowstone bison population. A frequency-dependent model of brucellosis transmission was superior to a density-dependent model in predicting out-of-sample observations of horizontal transmission probability. A mixture model including both transmission mechanisms converged on frequency dependence. Conditional on the frequency-dependent model, brucellosis median transmission rate was 1.87 yr À1 . The median of the posterior distribution of the basic reproductive ratio (R 0 ) was 1.75. Seroprevalence of adult females varied around 60% over two decades, but only 9.6 of 100 adult females were infectious. Brucellosis depressed recruitment; estimated population growth rate k averaged 1.07 for an infected population and 1.11 for a healthy population. We used five-year forecasting to evaluate the ability of different actions to meet management goals relative to no action. Annually removing 200 seropositive female bison increased by 30-fold the probability of reducing seroprevalence below 40% and increased by a factor of 120 the probability of achieving a 50% reduction in transmission probability relative to no action. Annually vaccinating 200 seronegative animals increased the likelihood of a 50% reduction in transmission probability by fivefold over no action. However, including uncertainty in the ability to implement management by representing stochastic variation in the number of accessible bison dramatically reduced the probability of achieving goals using interventions relative to no action. Because the width of the posterior predictive distributions of future population states expands rapidly with increases in the forecast horizon, managers must accept high levels of uncertainty. These findings emphasize the necessity of iterative, adaptive management with relatively short-term commitment to action and frequent reevaluation in response to new data and model forecasts. We believe our approach has broad applications.

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“…If a molecule is labelled, the rotation can be determined by measuring polarised light intensity in vertical and horizontal planes (19). It is host species-independent method and can be performed also with whole blood or milk (9,11,(20)(21)(22). According to OIE Terrestrial Manual 2009, the FPA is recommended as a prescribed test for bovine and porcine brucellosis for determining the health status of animals (19).…”

mentioning

confidence: 99%

Value of Fluorescence Polarisation Assay in Comparison to Traditional Techniques in Diagnosis of Porcine Brucellosis

Weiner

Iwaniak

Szulowski

2012

Bulletin of the Veterinary Institute in Pulawy

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The aim of this study was the evaluation of fluorescence polarisation assay (FPA) in the diagnosis of porcine brucellosis in comparison with Rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT), 2-mercaptoethanol test, and ELISA. Eight hundred seventeen sera from pigs, including 612 sera from healthy animals, seven sera from Brucella suis bv2 culture positive animals, and 198 sera classified as false positive, originated from confirmatory investigations, were used. All sera from healthy animals, negative in RBT, SAT, CFT, and ELISA were also negative in FPA. All sera positive in serological examination, originated from Brucella infected animals, were also positive in FPA. Among sera classified as false positive almost half of the samples tested (49.49%) reacted positively in FPA. The examinations confirmed the usefulness of FPA in diagnosis of porcine brucellosis, but the method, like the other tests, does not allow resolving the problem of discrimination cross-reacting from specific antibodies.

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Validation of the Fluorescence Polarization Assay and Comparison to Other Serological Assays for the Detection of Serum Antibodies to Brucella Abortus in Bison

Cited by 34 publications

References 11 publications

The Use of Fluorescence Polarization Assays for the Detection of Infectious Diseases

The Use of Fluorescence Polarization Assays for the Detection of Infectious Diseases

State‐space modeling to support management of brucellosis in the Yellowstone bison population

Value of Fluorescence Polarisation Assay in Comparison to Traditional Techniques in Diagnosis of Porcine Brucellosis

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