Validation of the drop coating deposition Raman method for protein analysis

Ortiz, Corasi; Zhang, Dongmao; Xie, Yong; Ribbe, Alexander E.; Ben‐Amotz, Dor

doi:10.1016/j.ab.2006.03.025

Cited by 81 publications

(134 citation statements)

References 24 publications

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“…This is unsuitable for most biological analytes, such as proteins, since the process of desiccation alters their configuration and hence their Raman spectrum [23]. Drop-coated deposition has been claimed to produce normal Raman spectra of proteins analogous to those obtained from aqueous proteins [24] by providing a "natural" environment. However, because protein structure is strongly dependent upon the hydration of the molecule, it is essential that aqueous conditions are maintained in order to perform rigorous investigation of biological proteins.…”

Section: Biophotonicsmentioning

confidence: 98%

Spectroscopy on the wing: Naturally inspired SERS substrates for biochemical analysis

Garrett¹,

Vukusic

Ogrin

et al. 2009

Journal of Biophotonics

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We show that naturally occurring chitinous nanostructures found on the wings of the Graphium butterfly can be used as substrates for surface-enhanced Raman scattering when coated with a thin film of gold or silver. The substrates were found to exhibit excellent biocompatibility and sensitivity, making them ideal for protein assaying. An assay using avidin/biotin binding showed that the substrates could be used to quantify protein binding directly from changes in the surface-enhanced Raman scattering (SERS) spectra and were sensitive over a concentration range comparable with a typical enzyme-linked immunosorbent assays (ELISA) assay. A biomimetic version of the wing nanostructures produced using a highly reproducible, large-scale fabrication process, yielded comparable enhancement factors and biocompatibility. The excellent biocompatibility of the wings and biomimetic substrates is unparalleled by other lithographically produced substrates, and this could pave the way for widespread application of ultrasensitive SERS-based bioassays.

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Section: Biophotonicsmentioning

confidence: 98%

Spectroscopy on the wing: Naturally inspired SERS substrates for biochemical analysis

Garrett¹,

Vukusic

Ogrin

et al. 2009

Journal of Biophotonics

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“…As a preconcentration treatment we used the so called drop coating deposition Raman ͑DCDR͒ method, 16 probing with the Raman microscope on the solid deposits left by the evaporation of a drop of the solution. This method introduces no unevenness between different points of the sample 17 and the preconcentration of the analyte makes the Raman analysis possible. In order to achieve good reproducibility in the Raman measurements, we used long acquisition times ͑from 20 to 30 min in the L-alanine experiments and from 1 to 2 min with the rhodamine-6G͒.…”

Section: Methodsmentioning

confidence: 99%

“…17 The flatness of the silicon surfaces was checked by AFM. Prior to DCDR deposition, in order to eliminate any contamination both substrates were rinsed with anhydrous ethanol ͑Sigma-Aldrich 459863͒.…”

Section: Methodsmentioning

confidence: 99%

Gold surface with gold nitride–a surface enhanced Raman scattering active substrate

Brieva

Alves

Krishnamurthy

et al. 2009

Journal of Applied Physics

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The nitration of gold surfaces is a nonpolluting method, which can lead to large scale production of substrates with remarkable properties and applications. We present a topographical study of the nanoscale structure of the gold nitride surfaces produced by radio frequency ͑rf͒ nitrogen plasma etching of thin gold films. Atomic force microscopy images taken after rf etching reveal the striking appearance of the cluster assembly with large clusters surrounded by small clusters ͑7.9Ϯ 1.4 and 2.3Ϯ 0.9 nm, respectively͒ appearing to exhibit an attractive interaction. We discuss the possible mechanism for this attraction based on a colloid model by Messina et al. ͓Phys. Rev. Lett. 85, 872 ͑2000͔͒. This surface exhibits a notable surface enhanced Raman scattering effect demonstrated with L-alanine and rhodamine-6G. The significance of this work is that we found that this SERS active gold nitride surface can be prepared in just one step: by nitrogen plasma etching a thin gold film. Until now most SERS active gold cluster covered surfaces have been prepared in several steps very often requiring complex lithography.

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“…Previous study has shown that protein and buffer species can segregate well upon deposition, especially for simple mixtures with relatively low concentrations. 11,16 However, compounds with a strong chemical affinity for each other are not expected to easily segregate. 17 A similar phenomenon has been observed in Raman spectra obtained from a dried synovial fluid droplet.…”

Section: Methodsmentioning

confidence: 99%

Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy

Huang

Sun²,

Wang

et al. 2013

J. Biomed. Opt

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Abstract. In this study, a rapid and simple method which combines drop coating deposition and Raman spectroscopy (DCDR) was developed to characterize the dry embryo culture media (ECM) droplet. We demonstrated that Raman spectra obtained from the droplet edge presented useful and characteristic signatures for protein and amino acids assessment. Using a different analytical method, scanning electron microscopy coupled with energy dispersive X-ray analysis, we further confirmed that Na, K, and Cl were mainly detected in the central area of the dry ECM droplet while sulphur, an indicative of the presence of macromolecules such as proteins, was mainly found at the periphery of the droplet. In addition, to reduce sample preparation time, different temperatures for drying the droplets were tested. The results showed that drying temperature at 50°C can effectively reduce the sample preparation time to 6 min (as compared to 50 min for drying at room temperature, ∼25°C) without inducing thermal damage to the proteins. This work demonstrated that DCDR has potential for rapid and reliable metabolomic profiling of ECM in clinical applications. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Validation of the drop coating deposition Raman method for protein analysis

Cited by 81 publications

References 24 publications

Spectroscopy on the wing: Naturally inspired SERS substrates for biochemical analysis

Spectroscopy on the wing: Naturally inspired SERS substrates for biochemical analysis

Gold surface with gold nitride–a surface enhanced Raman scattering active substrate

Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy

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