Validation of the cortisol method on the abbott architect® i2000 immunoassay system for measurement of urine cortisol by comparison to LC-MS-MS

Konforte, Danijela; Božović, Andrea; Mah, Wee Li; Martens, Pieter; Yip, P.

doi:10.1016/j.clinbiochem.2011.06.040

Cited by 2 publications

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“…The cortisol samples were centrifuged within 30 minutes of collection and then immediately stored in a freezer at −10°C or less until transported in a cooler box for analysis at a private laboratory outside the College of Medicine and Queen Elizabeth Central Hospital. Cortisol samples were analyzed using chemiluminescent microparticle immunoassay technology using ARCHITECT Cortisol assay (Abbot Laboratory, Abbot Park, IL) 22 to quantitatively determine the serum cortisol. A cortisol level of < 25 μg/dL (690 nmol/L) was considered low, 14 , 23 and a cortisol level < 10 μg/dL (276 nmol/L) was considered adrenal insufficiency.…”

Section: Methodsmentioning

confidence: 99%

The Association of Low Blood Glucose and Low Serum Cortisol Levels in Severely Ill Children Admitted to Tertiary Referral Hospitals in Malawi: A Case-Control Study

Ngwalangwa

Katumbi

Dube

et al. 2021

The American Journal of Tropical Medicine and Hygiene

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Low blood glucose concentrations (< 5 mmol/L) in severely ill children presenting to hospitals in low-income countries are associated with mortality. Adrenal insufficiency with low cortisol levels may contribute to low blood glucose concentrations. Understanding the association between low cortisol and low blood glucose may assist in improving guidelines for management of severely ill children. The study aimed to determine the association between low serum cortisol and low blood glucose in severely ill children. A matched case-control study of children aged 1 month to 15 years was conducted at two tertiary hospitals in Malawi. Cases were children with blood glucose <5 mmol/L. Two age-matched controls with blood glucose of ≥5–15 mmol/L were enrolled per case. Low cortisol was defined as serum cortisol of <25 µg/dL (690 nmol/L) and adrenal insufficiency as serum cortisol of <10 µg/dL (276 nmol/L). A total of 54 cases and 108 controls were enrolled with, median age of 2.8 years (interquartile range [IQR]: 1.7–4.4). The median cortisol level was 58.7 µg/dL (IQR: 42.3–61.8) in cases and 40.9 µg/dL (IQR: 33.7–51.2) in controls (P = 0.911). The proportion of low cortisol was 4/54 (7.4%) in cases and 9/108 (8.3%) in controls. Logistic regression shows no association between low cortisol and low blood glucose (adjusted odds ratio: 0.33; 95% confidence interval, 0.04–3.02). Results suggest that there is no association between low cortisol and low blood glucose among severely ill children presenting to hospitals in Malawi. The reason for low blood glucose needs further investigation.

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Section: Methodsmentioning

confidence: 99%

The Association of Low Blood Glucose and Low Serum Cortisol Levels in Severely Ill Children Admitted to Tertiary Referral Hospitals in Malawi: A Case-Control Study

Ngwalangwa

Katumbi

Dube

et al. 2021

The American Journal of Tropical Medicine and Hygiene

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“…The former has higher analytical specificity for UFC and is recommended as a reference method [11]. UFC immunoassays have varying degrees of agreement with LC-MS/MS (correlation coefficients range from 0.57 to 0.84) [9,12,13]. The cortisol assay of Abbott Architect i2000SR has the highest analytical consistency with LC-MS/MS [9].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Direct and Extraction Immunoassay Methods With Liquid Chromatography-Tandem Mass Spectrometry Measurement of Urinary Free Cortisol for the Diagnosis of Cushing’s Syndrome

Mu,

Fang,

et al. 2023

Ann Lab Med

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Background: Twenty-four-hour urinary free cortisol (UFC) measurement is the initial diagnostic test for Cushing's syndrome (CS). We compared UFC determination by both direct and extraction immunoassays using Abbott Architect, Siemens Atellica Solution, and Beckman DxI800 with liquid chromatography-tandem mass spectrometry (LC-MS/MS). In addition, we evaluated the value of 24-hr UFC measured by six methods for diagnosing CS.Methods: Residual 24-hr urine samples of 94 CS and 246 non-CS patients were collected. A laboratory-developed LC-MS/MS method was used as reference. UFC was measured by direct assays (D) using Abbott, Siemens, and Beckman platforms and by extraction assays (E) using Siemens and Beckman platforms. Method was compared using Passing-Bablok regression and Bland-Altman plot analyses. Cut-off values for the six assays and corresponding sensitivities and specificities were calculated by ROC analysis.Results: Abbott-D, Beckman-E, Siemens-E, and Siemens-D showed strong correlations with LC-MS/MS (Spearman coefficient r = 0.965, 0.922, 0.922, and 0.897, respectively), while Beckman-D showed weaker correlation (r = 0.755). All immunoassays showed proportionally positive bias. The areas under the curve were 0.975 for Abbott-D, 0.972 for LC-MS/MS, 0.966 for Siemens-E, 0.948 for Siemens-D, 0.955 for Beckman-E, and 0.877 for Beckman-D. The cut-off values varied significantly (154.8-1,321.5 nmol/24 hrs). Assay sensitivity and specificity ranged from 76.1% to 93.2% and from 93.0% to 97.1%, respectively.Conclusions: Commercially available immunoassays for measuring UFC show different levels of analytical consistency compared to LC-MS/MS. Abbott-D, Siemens-E, and Beckman-E have high diagnostic accuracy for CS.

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Validation of the cortisol method on the abbott architect® i2000 immunoassay system for measurement of urine cortisol by comparison to LC-MS-MS

Cited by 2 publications

References 0 publications

The Association of Low Blood Glucose and Low Serum Cortisol Levels in Severely Ill Children Admitted to Tertiary Referral Hospitals in Malawi: A Case-Control Study

The Association of Low Blood Glucose and Low Serum Cortisol Levels in Severely Ill Children Admitted to Tertiary Referral Hospitals in Malawi: A Case-Control Study

Comparison of Direct and Extraction Immunoassay Methods With Liquid Chromatography-Tandem Mass Spectrometry Measurement of Urinary Free Cortisol for the Diagnosis of Cushing’s Syndrome

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