Validation of the combined ATR-FTIR/tape stripping technique for monitoring the distribution of surfactants in the stratum corneum

Hoppel, Magdalena; Baurecht, Dieter; Holper, Evelyn; Mahrhauser, Denise-Silvia; Valenta, Claudia

doi:10.1016/j.ijpharm.2014.06.011

Cited by 29 publications

(17 citation statements)

References 23 publications

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“…Regarding the amide I/amide II absorbance ratio at the skin surface, significantly higher values were obtained after waxing and the depilatory cream; previous groups have associated such changes with an increased moisture content of the SC [29,39]. For our data, this observation would be in good agreement with the capacitive contact imaging data, where a visual tendency towards uniformly higher mean permittivity values was observed after waxing and the depilatory cream.…”

Section: Atr-ftir Spectroscopy and Sc Compoundssupporting

confidence: 91%

“…To assess the amount of skin lipids, the symmetric CH 2 /amide II absorbance ratio was compared between control (scissors) and samples treated with the investigated hair removal methods [29,30]. The wavenumber limits for integration were 2,861-2,837 cm -1 for the symmetric CH 2 absorbance and 1,585-1,487 cm -1 for the amide II absorbance.…”

Section: Atr-ftir Spectroscopymentioning

confidence: 99%

“…For evaluation of changes in the amide I absorbance, caused by applied substances or treatments, the amide I/amide II absorbance ratio was analysed [29,30]. The wavenumber limits for integration were 1,707-1,590 cm -1 for the amide I absorbance and 1,585-1,487 cm -1 for the amide II absorbance.…”

Section: Atr-ftir Spectroscopymentioning

confidence: 99%

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Effect of Physical and Chemical Hair Removal Methods on Skin Barrier Function in vitro: Consequences for a Hydrophilic Model Permeant

Pany

Klang²,

Brunner

et al. 2018

Skin Pharmacol Physiol

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Background: Although very common in our society, the effect of hair removal on physiological skin parameters and on the ingress of applied chemicals has not been systematically investigated. Thus, as a first step, the aim of the present study was to elucidate the effect of hair removal through epilation (electric epilation, waxing) and depilation (dry and wet shaving, depilatory cream) on skin properties in vitro using the porcine ear model. Methods: Attenuated total reflection Fourier transform infrared spectroscopy, measurement of the transepidermal water loss (TEWL), visualization by capacitance-based contact imaging, confocal Raman spectroscopy (CRS), diffusion cell studies and tape stripping experiments were employed. Results: Increased TEWL and altered skin permittivity maps were observed. Decreased stratum corneum thickness was observed after waxing. Diffusion cell studies showed increased skin permeation especially in case of dry shaving, electric epilation and waxing. Conclusion: Considering CRS and diffusion cell data, a moderate if significant decrease in skin barrier function was found after hair removal by dry shaving (physical skin/material interaction) and epilation methods (plucking out the entire hair, for example, by electrical epilation and waxing). Subsequent experiments will include testing of different permeants covering a broad range of physicochemical properties in vitro and confirming our findings in vivo.

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Section: Atr-ftir Spectroscopy and Sc Compoundssupporting

confidence: 91%

Section: Atr-ftir Spectroscopymentioning

confidence: 99%

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Effect of Physical and Chemical Hair Removal Methods on Skin Barrier Function in vitro: Consequences for a Hydrophilic Model Permeant

Pany

Klang²,

Brunner

et al. 2018

Skin Pharmacol Physiol

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“…Recently, the penetration of SLES into the SC was investigated by our working group [13]. Since one of the aims of the present study was to confirm less skin irritation potential of natural surfactants (APG and SL) compared to anionic surfactants (SDS and SLES), we included the obtained SLES data in this work (fig.…”

Section: Resultsmentioning

confidence: 99%

Monitoring the Distribution of Surfactants in the Stratum Corneum by Combined ATR-FTIR and Tape-Stripping Experiments

Hoppel¹,

Holper²,

Baurecht

et al. 2015

Skin Pharmacol Physiol

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Combined ATR-FTIR (attenuated total reflection-Fourier transform infrared) spectroscopy and tape-stripping experiments in vitro on porcine ear skin were used to investigate the spatial distribution of different surfactants in the stratum corneum (SC). To reveal a possible connection between the size of the formed micelles and skin penetration, dynamic light-scattering measurements of the aqueous surfactant solutions were also taken. Compared to an alkyl polyglycoside and sucrose laurate, a deeper skin penetration of the anionic surfactants sodium dodecyl sulfate (SDS) und sodium lauryl ether sulfate (SLES) could be related to a smaller size of the formed micelles. Beside the differences in spatial distribution, a link between the physical presence of anionic surfactants in the SC and a decrease of skin hydration was found. Furthermore, the incorporation of SDS and SLES into the SC, even after a brief, consumer-orientated washing procedure with commercially available hair shampoos, was confirmed.

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“…The skin bands obtained from skin intercellular lipids and keratin (corneocytes) were as follows: the modes were associated with protein as amide I (C=O) vibration (at approximately 1,640 cm . 54,55 In Figure 7, it can be clearly seen that the skin subjected to a stationary magnetic field shows a similar spectrum to the control. However, after exposure to an alternating magnetic field, the symmetric and asymmetric methylene stretching bands were shifted to lower values as shown in Figure 7B.…”

mentioning

confidence: 78%

Study of magnetic silk fibroin nanoparticles for massage-like transdermal drug delivery

Chen¹,

Chen²,

Wang³

et al. 2015

IJN

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A synergistic approach by the combination of magnetic nanoparticles with an alternating magnetic field for transdermal drug delivery was investigated. Methotrexate-loaded silk fibroin magnetic nanoparticles were prepared using suspension-enhanced dispersion by supercritical CO 2 . The physiochemical properties of the magnetic nanoparticles were characterized. In vitro studies on drug permeation across skin were performed under different magnetic fields in comparison with passive diffusion. The permeation flux enhancement factor was found to increase under a stationary magnetic field, while an alternating magnetic field enhanced drug permeation more effectively; the combination of stationary and alternating magnetic fields, which has a massage-like effect on the skin, achieved the best result. The mechanistic studies using attenuated total reflection Fourier-transform infrared spectroscopy demonstrate that an alternating magnetic field can change the ordered structure of the stratum corneum lipid bilayers from the gel to the lipid-crystalline state, which can increase the fluidity of the stratum corneum lipids, thus enhancing skin penetration. Compared with the other groups, the fluorescence signal with a bigger area detected in deeper regions of the skin also reveals that the simulated massage could enhance the drug permeation across the skin by increasing the follicular transport. The combination of magnetic nanoparticles with stationary/alternating magnetic fields has potential for effective massage-like transdermal drug delivery.

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Validation of the combined ATR-FTIR/tape stripping technique for monitoring the distribution of surfactants in the stratum corneum

Cited by 29 publications

References 23 publications

Effect of Physical and Chemical Hair Removal Methods on Skin Barrier Function in vitro: Consequences for a Hydrophilic Model Permeant

Effect of Physical and Chemical Hair Removal Methods on Skin Barrier Function in vitro: Consequences for a Hydrophilic Model Permeant

Monitoring the Distribution of Surfactants in the Stratum Corneum by Combined ATR-FTIR and Tape-Stripping Experiments

Study of magnetic silk fibroin nanoparticles for massage-like transdermal drug delivery

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