Validation of the CDC biofilm reactor as a dynamic model for assessment of encrustation formation on urological device materials

Gilmore, Brendan; Hamill, T.M.; Jones, David S.; Gorman, Seán

doi:10.1002/jbm.b.31567

Cited by 38 publications

(34 citation statements)

References 43 publications

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“…With these model systems, bacterial cells from an inoculum suspension are first allowed to adhere onto the substrate surface. After the adhesion phase, a flow of growth medium is maintained to allow bacterial growth and biofilm formation (Gilmore et al, 2010; Nava‐Ortiz et al, 2010). While these models are suitable for studying biofilm formation in situ on a particular substrate surface, it may not closely simulate biofilm formation behavior on an infected device surface caused by a highly motile pathogen.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of escherichia coli and proteus mirabilis adhesion and biofilm formation on medical grade silicone surface

Wang¹,

Neoh²,

Shi³

et al. 2011

Biotech & Bioengineering

135

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Silicone has been utilized extensively for biomedical devices due to its excellent biocompatibility and biodurability properties. However, its surface is easily colonized by bacteria which will increase the probability of nosocomial infection. In the present work, a hydrophilic antimicrobial carboxymethyl chitosan (CMCS) layer has been grafted on medical grade silicone surface pre-treated with polydopamine (PDA). The increase in hydrophilicity was confirmed from contact angle measurement. Bacterial adhesion tests showed that the PDA-CMCS coating reduced the adhesion of Escherichia coli and Proteus mirabilis by ≥ 90%. The anti-adhesion property was preserved even after the aging of the functionalized surfaces for 21 days in phosphate-buffered saline (PBS), and also after autoclaving at 121°C for 20 min. Both E. coli and P. mirabilis readily form biofilms on the pristine surface under static and flow conditions but with the PDA-CMCS layer, biofilm formation is inhibited. The flow experiments indicated that it is more difficult to inhibit biofilm formation by the highly motile P. mirabilis as compared to E. coli. No significant cytotoxicity of the modified substrates was observed with 3T3 fibroblasts.

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Section: Resultsmentioning

confidence: 99%

Inhibition of escherichia coli and proteus mirabilis adhesion and biofilm formation on medical grade silicone surface

Wang¹,

Neoh²,

Shi³

et al. 2011

Biotech & Bioengineering

135

View full text Add to dashboard Cite

show abstract

“…Different superscript letters indicate significant differences (Tukey's HSD, p ≤ 0.05) between the means of different surfaces. (1.13 ± 0.18) × 10 7 a (9.28 ± 3.51) × 10 6 a (3.39 ± 0.35) × 10 7 a (3.57 ± 0.92) × 10 6 c lDPE-CA (4.05 ± 1.07) × 10 6 b (4.40 ± 0.70) × 10 5 c (4.13 ± 0.46) × 10 7 b (4.69 ± 0.28) × 10 6 c lDPE-SA (4.72 ± 0.52) × 10 6 b (5.12 ± 0.91) × 10 5 c (4.16 ± 0.29) × 10 7 b (3.84 ± 0.38) × 10 6 c Ethanol (20 min) Coupon Bulk liquid PBS EtoH PBS EtoH lDPE (1.07 ± 0.13) × 10 7 a (1.00 ± 0.04) × 10 7 a (3.39 ± 0.34) × 10 7 a (1.87 ± 0.08) × 10 6 b lDPE-CA (3.32 ± 0.32) × 10 6 b (1.16 ± 0.16) × 10 6 c (4.13 ± 0.60) × 10 7 a (5.39 ± 0.50) × 10 6 b lDPE-SA (4.41 ± 0.78) × 10 6 b (1.44 ± 0.15) × 10 6 c (4.16 ± 0.51) × 10 7 a (9.53 ± 0.17) × 10 6 b A laboratory standard procedure using a CDC biofilm reactor was employed to grow E. coli biofilms, simulating the conditions to which surfaces of a wide range of applications are subjected during their use (Goeres et al 2005;Gilmore et al 2010;Williams et al 2011). Experiments revealed that biofilms were significantly affected when grown on both LDPE-CA and LDPE-SA in comparison to the LDPE surface.…”

Section: Discussionmentioning

confidence: 99%

Zosteric acid and salicylic acid bound to a low density polyethylene surface successfully control bacterial biofilm formation

et al. 2018

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The active moieties of the anti-biofilm natural compounds zosteric (ZA) and salicylic (SA) acids have been covalently immobilized on a low density polyethylene (LDPE) surface. The grafting procedure provided new non-toxic eco-friendly materials (LDPE-CA and LDPE-SA) with anti-biofilm properties superior to the conventional biocide-based approaches and with features suitable for applications in challenging fields where the use of antimicrobial agents is limited. Microbiological investigation proved that LDPE-CA and LDPE-SA: (1) reduced Escherichia coli biofilm biomass by up to 61% with a mechanism that did not affect bacterial viability; (2) significantly affected biofilm morphology, decreasing biofilm thickness, roughness, substratum coverage, cell and matrix polysaccharide bio-volumes by >80% and increasing the surface to bio-volume ratio; (3) made the biofilm more susceptible to ampicillin and ethanol. Since no molecules were leached from the surface, they remained constantly effective and below the lethal level; therefore, the risk of inducing resistance was minimized.

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“…The use of flow systems to study infection stone formation is promising, as they can model the hydraulic conditions in the urinary tract for extended periods of time. For instance, the Center for Disease Control (CDC) biofilm reactor 158 has been described to yield valuable and reproducible results related to the encrustation of urological device materials 189 . This reactor enables the growth of bacterial biofilms on removable coupons under controlled conditions to evaluate the effects of bacterial species, modulators of struvite crystallization, and urine chemistry on biomineralization.…”

Section: Multidisciplinary Approach To Managementmentioning

confidence: 99%

Current insights into the mechanisms and management of infection stones

et al. 2018

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Infection stones are complex aggregates of crystals amalgamated in an organic matrix that are strictly associated with urinary tract infections. The management of patients who form infection stones is challenging owing to the complexity of the calculi and high recurrence rates. The formation of infection stones is a multifactorial process that can be driven by urine chemistry , the urine microenvironment, the presence of modulator substances in urine, associations with bacteria, and the development of biofilms. Despite decades of investigation, the mechanisms of infection stone formation are still poorly understood. A mechanistic understanding of the formation and growth of infection stones-including the role of organics in the stone matrix, microorganisms, and biofilms in stone formation and their effect on stone characteristics-and the medical implications of these insights might be crucial for the development of improved treatments. Tools and approaches used in various disciplines (for example, engineering, chemistry , mineralogy , and microbiology) can be applied to further understand the microorganism-mineral interactions that lead to infection stone formation. Thus, the use of integrated multidisciplinary approaches is imperative to improve the diagnosis, prevention, and treatment of infection stones.

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Validation of the CDC biofilm reactor as a dynamic model for assessment of encrustation formation on urological device materials

Cited by 38 publications

References 43 publications

Inhibition of escherichia coli and proteus mirabilis adhesion and biofilm formation on medical grade silicone surface

Inhibition of escherichia coli and proteus mirabilis adhesion and biofilm formation on medical grade silicone surface

Zosteric acid and salicylic acid bound to a low density polyethylene surface successfully control bacterial biofilm formation

Current insights into the mechanisms and management of infection stones

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