Validation of the CALUX bioassay as a screening and semi-quantitative method for PCDD/F levels in cow's milk

Chou, I-Cheng; Lee, Wen-Jhy; Wang, Lin‐Chi; Chang-Chien, Guo-Ping; Lee, Wei-Shan; Lee, Hsinyu

doi:10.1016/j.jhazmat.2007.11.023

Cited by 20 publications

(17 citation statements)

References 15 publications

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“…Generally, CALUX measurements are higher for the PCDD/F fraction [3, 5] and lower for the dl-PCB fraction [27] when compared to GC-HRMS. Chou et al [3] calculated CALUX/GC-HRMS ratios for the PCDD/Fs between 1.9 and 2.4 in cow ‘s milk samples, while Van Overmeire et al [5] found median CALUX/GC-HRMS ratios of 1.58 and 0.26 for the PCDD/Fs and dl-PCBs, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Chou et al [3] calculated CALUX/GC-HRMS ratios for the PCDD/Fs between 1.9 and 2.4 in cow ‘s milk samples, while Van Overmeire et al [5] found median CALUX/GC-HRMS ratios of 1.58 and 0.26 for the PCDD/Fs and dl-PCBs, respectively. Carbonnelle et al [27] suggested a three-fold underestimation of the dl-PCB concentration in different matrices when using a mouse hepatoma CALUX bioassay.…”

Section: Resultsmentioning

confidence: 99%

“…However, so far most validated CALUX methods for quantification of PCDD/Fs and dl-PCBs in human and bovine milk samples have also used relatively large volumes. Some researchers used 60 mL milk [3–5], while others used 20 mL [6] or 10 mL milk [7–9]. Since in this Flemish human milk campaign not only PCDD/Fs and dioxin-like PCB were analyzed, but also other POPs (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Determination of PCDD/Fs, PBDD/Fs and dioxin-like PCBs in human milk from mothers residing in the rural areas in Flanders, using the CALUX bioassay and GC-HRMS

Croes

Colles

Koppen

et al. 2013

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Since the CALUX (Chemically Activated LUciferase gene eXpression) bioassay is a fast and inexpensive tool for the determination of dioxin-like compounds in a large number of samples and requires only small sample volumes, the use of this technique in human biomonitoring programs provides a good alternative to GC-HRMS. In this study, a new CALUX method for the separate analysis of PCDD/Fs and dioxin-like PCBs (dl-PCBs) in small amounts of human milk samples with the new sensitive H1L7.5c1 cell line was used to analyze 84 human milk samples, collected from mothers residing in the Flemish rural communities. The geometric mean CALUX-Bioanalytical Equivalent (CALUX-BEQ) values, reported for the 84 mothers from the study area were 10.4 (95% CI: 9.4–11.4) pg CALUX-BEQ per g lipid or 0.41 (95% CI: 0.37–0.45) pg CALUX-BEQ per g milk for the PCDD/Fs and 1.73 (1.57–1.91) pg CALUX-BEQ per g lipid or 0.07 (95% CI: 0.06–0.08) pg CALUX-BEQ per g milk for the dioxin-like PCBs. Multiple regression analysis showed significant associations between PCDD/Fs and weight change after pregnancy, smoking and consumption of local eggs. One pooled human milk sample was analyzed with both CALUX and GC-HRMS. The ratio of CALUX and GC-HRMS results for this sample were respectively 1.60, 0.58 and 1.23 for the PCDD/Fs, the dl-PCBs and the sum of both fractions, when using the 2005-TEF values. Additionally, also low levels of certain brominated dioxins and furans were detected in the pooled sample with GC-HRMS.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of PCDD/Fs, PBDD/Fs and dioxin-like PCBs in human milk from mothers residing in the rural areas in Flanders, using the CALUX bioassay and GC-HRMS

Croes

Colles

Koppen

et al. 2013

Talanta

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“…The 2,3,7,8-TCDD calibration curve for the CALUX bioassay was established from the relationship between the concentrations of the 2,3,7,8-TCDD standard solutions (250, 125, 62.5, 31.3, 15.6, 7.81, 3.91, 1.95, 0.977, 0.488, and 0.244 pg-TCDD/mL medium), as shown in our previous study [19]. For the tests to be significant, two criteria must be met.…”

Section: Resultsmentioning

confidence: 99%

Metal interference on luciferase activity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in bioassays of recombinant mouse hepatoma cells

Chou

Wang

et al. 2009

Journal of Hazardous Materials

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“…The H1L7.5c1 cell line was specially designed to analyze low concentrations of PCDD/Fs and PCBs in small sample volumes. With the less sensitive H1L6.1 cell line, which was commonly used in previous biomonitoring studies [7, 8, 9] and food/feed analysis [10, 11], only a single-point analysis of the whole extract was often used and it was not possible to measure the dioxin-like PCB fraction, since most samples were below the quantification limit (LOQ) [9]. In this study, for the first time, dioxin-like PCBs could be measured in serum samples with the improved H1L7.5c1 cell line with a high percentage of the samples above the LOQ.…”

Section: Introductionmentioning

confidence: 99%

Quantification of PCDD/Fs and dioxin-like PCBs in small amounts of human serum using the sensitive H1L7.5c1 mouse hepatoma cell line: Optimization and analysis of human serum samples from adolescents of the Flemish Environment and Health Study (FLEHS II)

Croes

Langenhove

Hond

et al. 2011

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Since the CALUX (Chemically Activated LUciferase gene eXpression) bioassay is a fast and inexpensive tool for the throughput analysis of dioxin-like compounds in a large number of samples and requires only small sample volumes, the use of this technique in human biomonitoring programs provides a good alternative to GC-HRMS. In this study, a method for the separate analysis of PCDD/Fs and dioxin-like PCBs (dl-PCBs) in human serum with the new sensitive H1L7.5c1 mouse hepatoma cell line was optimized. Sample dilution factors of 5 and 2.4 were selected for routine analysis of respectively the PCDD/Fs and dl-PCBs. The validation studies showed that repeatability and within-lab reproducibility for the QC standard were within the in-house criteria. A long-term within-lab reproducibility of 25% for the PCDD/F fraction and 41% for the dl-PCB fraction for the analysis of pooled serum samples, expressed as pg BEQ/g fat, was determined. CALUX recoveries of the spiked procedural blanks were within the acceptable in-house limits of 80–120% for both fractions and the LOQ was 30.3 pg BEQ/g fat for the PCDD/Fs and 14.5 pg BEQ/g fat for the dl-PCBs. The GC-HRMS recovery of a C13-spiked pooled serum sample was between 60–90 % for all PCDD/F congeners and between 67–82 % for the non-ortho PCBs. An adequate separation between both fractions was found. The CALUX/GC-HRMS ratio for a pooled serum sample was respectively 2.0 and 1.4 for the PCDD/Fs and the dl-PCBs, indicating the presence of additional AhR active compounds. As expected, a correlation was found between human serum samples analyzed with both the new H1L7.5c1 cell line and the more established H1L6.1c3 cell line. The geometric mean CALUX-BEQ values, reported for the adolescents of the second Flemish Environment and Health Study (FLEHS II) recruited in 2009–2010, were 108 (95% CI: 101–114) pg CALUX-BEQ/g fat for the PCDD/Fs and 32.1 (30.1–34.2) pg CALUX-BEQ/g fat for the dioxin-like PCBs.

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Validation of the CALUX bioassay as a screening and semi-quantitative method for PCDD/F levels in cow's milk

Cited by 20 publications

References 15 publications

Determination of PCDD/Fs, PBDD/Fs and dioxin-like PCBs in human milk from mothers residing in the rural areas in Flanders, using the CALUX bioassay and GC-HRMS

Determination of PCDD/Fs, PBDD/Fs and dioxin-like PCBs in human milk from mothers residing in the rural areas in Flanders, using the CALUX bioassay and GC-HRMS

Metal interference on luciferase activity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in bioassays of recombinant mouse hepatoma cells

Quantification of PCDD/Fs and dioxin-like PCBs in small amounts of human serum using the sensitive H1L7.5c1 mouse hepatoma cell line: Optimization and analysis of human serum samples from adolescents of the Flemish Environment and Health Study (FLEHS II)

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