Validation of the Analytical Procedure for the Determination of Malondialdehyde and Three Other Aldehydes in Vegetable Oil Using Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC-MS/MS) and Application to Linseed Oil

Douny, Caroline; Tihon, Angélique; Bayonnet, Pierre; Brose, François; Degand, Guy; Rozet, Eric; Milet, Jérôme; Ribonnet, Laurence; Lambin, Loranne; Larondelle, Yvan; Scippo, Marie‐Louise

doi:10.1007/s12161-014-0028-z

Cited by 55 publications

(43 citation statements)

References 40 publications

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“…MDA, HHE, and HNE have attracted attention in some regions: the Belgian Superior Health Council has considered MDA and HNE as major concerns for human health; HNE has been recognized as a potential public health concern in America; and the European Food Safety Authority scientific committee has suggested the threshold of toxicological concern (TTC) level of exposure for MDA, HHE, and HNE as 30, 1.5, and 1.5 μg kg −1 bw/day, respectively . Remarkably, the number of scientific articles reporting MDA, HHE, and HNE as related to the food domain are very few (Table S1, Supporting Information).…”

Section: Introductionmentioning

confidence: 99%

“…Three articles discussed the formation of MDA, HHE, and HNE during the dynamic gastrointestinal in vitro digestion of cod liver oil or fish oil . Apart from two other articles focusing on the simultaneous determination of MDA, HHE, and HNE in vegetable oils, only one published paper investigated the formation of the target aldehydes . However, the authors mainly paid attention to the oxidation of ω‐3 PUFA in tuna oil.…”

Section: Introductionmentioning

confidence: 99%

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Malondialdehyde, 4‐Hydroxy‐2‐Hexenal, and 4‐Hydroxy‐2‐Nonenal in Vegetable Oils: Formation Kinetics and Application as Oxidation Indicators

Liu

2019

Euro J Lipid Sci & Tech

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The formation of three toxic reactive aldehydes, malondialdehyde (MDA), 4‐hydroxy‐hexenal (HHE), and 4‐hydroxy‐2‐nonenal (HNE), is investigated in five commonly consumed vegetable oils using a method based on the Schaal oven test. HHE is only detected in linseed oil (LO) and rapeseed oil (RO), while MDA and HNE are found to be present in all tested oils. The formation potential of MDA, HHE, and HNE in the tested oils is closely related to the initial polyunsaturated fatty acid (PUFA) content in the oil. Correlation analyses reveal low correlation coefficients for MDA, determining its unsuitability to evaluate the oxidation of oils with low PUFA content, such as palm oil and camellia oil; in contrast, HHE and HNE show sufficient correlations with other oxidation indices in all tested oils. Furthermore, regression analysis indicates that HHE content can predict the oxidation of ω‐3 type oil, while HNE content can estimate oxidative deterioration of ω‐6 type oils. Particularly, HNE content can also evaluate the oxidation of ω‐9 type oils such as PO and CLO with relatively low PUFA level for the high correlations (>0.991, P < 0.01) toward the total oxidation value (TOTOX). Practical Applications: Edible oil consumption continues to increase worldwide. Therefore, it is very important to understand the conditions under which potentially health‐damaging compounds are formed in these oils and to develop methods to inhibit their formation or eliminate them from the oils. This study investigates the formation of three toxically reactive aldehydes, namely MDA, HHE, and HNE, through lipid oxidation, specifically PUFA peroxidation. The application of these aldehydes as oxidation indicators for different vegetable oils is also explored. This work could prove beneficial for the quality control of vegetable oils in the edible oil industry. The formation of three toxic aldehydes, MDA, HHE, and HNE in different types of vegetable oils is investigated. Considerable amounts of MDA, HHE, and HNE is found in all the tested oils. The results show the formation of these three aldehydes is competitive. Besides, MDA content is not appropriate to evaluate the oxidation of oils with low PUFA content, such as PO and CLO; while HHE and HNE can characteristically depict the oxidation of ω‐3 and ω‐6 type oil.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Malondialdehyde, 4‐Hydroxy‐2‐Hexenal, and 4‐Hydroxy‐2‐Nonenal in Vegetable Oils: Formation Kinetics and Application as Oxidation Indicators

Liu

2019

Euro J Lipid Sci & Tech

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“…Malondialdehyde and conjugated diene are secondary oxidation products formed as a result of the oxidation of PUFA. The colorimetric method (TBARS) used to quantify aldehydes provides is a good indicator of the level of oxidation in rapeseed oil, but lacks specificity because it measures the total content of aldehydes able to react with thiobarbituric acid, expressed in malondialdehyde content (Douny et al ., ). Results from changes in MDA and CD values in rapeseed oil during the accelerated oxidation process and the effect of rosemary addition are shown in Fig.…”

Section: Resultsmentioning

confidence: 97%

Rosemary powder filtrate improves the oxidative stability and antioxidant properties of rapeseed oil: potential applications for domestic cooking

Redondo‐Cuevas

Hayes

Nicol

et al. 2018

Int J of Food Sci Tech

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Summary Rapeseed oil is an important source of edible oil for the human diet but is also highly susceptible to oxidative deterioration. Previous research has demonstrated that rosemary powder and its extracts can increase the oxidative stability of rapeseed oil. In this study, the protective effect of ground rosemary powder (0.25–2.0%) against the oxidative degradation of filtered rapeseed oil was assessed. Rapeseed oil filtrate showed enhanced antioxidant capacity and higher induction periods (IP) based on Rancimat analysis. Lower conductivity changes and total colour differences, reduced malondialdehyde and conjugated dienes content, and higher levels of tocopherols and carotenoids were observed for the oils formulated with rosemary powder after 1.5 and 3 h of exposure to accelerated oxidation. The use of rosemary powder filtrate could be an effective way to protect rapeseed oil from oxidation during storage or cooking with domestic and commercial applications.

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“…The method used in the present study was adapted from earlier work where 4 aldehydes (MDA, 4‐HNE, 4‐HHE and 2,4‐decadienal) were analyzed in linseed oil, using MeMDA as the internal standard . Four aldehydes (CRT, HXL, BNZ and 2,4‐nonadienal) and three internal standards (methylcrotonaldehyde (MeCRT), benzaldehyde‐ 13 C, hexanal‐D 12 and 4‐HNE‐D 3 ) were added to the previously developed method for oil (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…The best compromise between solubility and chromatographic peak areas was obtained for the 8 aldehydes with a DNPH concentration of 0.05 M in acetonitrile/acetic acid (9/1, v/v ). After checking that they were still suitable, the derivatization time and temperature were maintained at 60°C for 2 h, as previously optimized …”

Section: Resultsmentioning

confidence: 99%

Development of an LC‐MS/MS analytical method for the simultaneous measurement of aldehydes from polyunsaturated fatty acids degradation in animal feed

Douny

Bayram

Brose

et al. 2016

Drug Testing and Analysis

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Knowing that polyunsaturated fatty acids can lead to the formation of potentially toxic aldehydes as secondary oxidation products, an analytical method using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) detection was developed to measure the concentration of eight aldehydes in animal feed: malondialdehyde (MDA), 4-hydroxy-2-nonenal (4-HNE), 4-hydroxy-2-hexenal (4-HHE), crotonaldehyde (CRT), benzaldehyde (BNZ), hexanal (HXL), 2,4-nonadienal, and 2,4-decadienal. The developed method was validated according to the criteria and procedure described in international standards. The evaluated parameters were specificity/selectivity, recovery, precision, accuracy, uncertainty, limits of detection and quantification, using the concept of accuracy profiles. These parameters were determined during experiments conducted over three different days with ground Kellogg's® Corn Flakes® cereals as model matrix for animal feed and spiked at different levels of concentration. Malondialdehyde, 4-HHE, 4-HNE, crotonaldehyde, benzaldehyde, and hexanal can be analyzed in the same run in animal feed with a very good accuracy, with recovery rates ranging from 86 to 109% for a working range going from 0.16 to 12.50 mg/kg. The analysis of 2,4-nonadienal and 2,4-decadienal can also be performed but in a limited range of concentration and with a limited degree of accuracy. Their recovery rates ranged between 54 and 114% and coefficient of variation for the intermediate precision between 11 and 25% for these two compounds.

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Validation of the Analytical Procedure for the Determination of Malondialdehyde and Three Other Aldehydes in Vegetable Oil Using Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC-MS/MS) and Application to Linseed Oil

Cited by 55 publications

References 40 publications

Malondialdehyde, 4‐Hydroxy‐2‐Hexenal, and 4‐Hydroxy‐2‐Nonenal in Vegetable Oils: Formation Kinetics and Application as Oxidation Indicators

Malondialdehyde, 4‐Hydroxy‐2‐Hexenal, and 4‐Hydroxy‐2‐Nonenal in Vegetable Oils: Formation Kinetics and Application as Oxidation Indicators

Rosemary powder filtrate improves the oxidative stability and antioxidant properties of rapeseed oil: potential applications for domestic cooking

Development of an LC‐MS/MS analytical method for the simultaneous measurement of aldehydes from polyunsaturated fatty acids degradation in animal feed

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