“…The inclusion criteria are as follows: (1) being willing to practice appropriate birth control, (2) being willing to eat at Pennington Biomedical Research Center (PBRC) at least 3 times per week, (3) beingwilling to consume alcohol (EtOH group), (4) being willing to abstain from alcohol (CTL group), and (5) being a daily or almost daily drinker, defined as typically consuming at least 8 drinks per week, but no more than 4 per day. Exclusion criteria included but are not limited to: (1) non-drinkers of alcohol, (2) habitual binge drinkers, defined by the consumption of ≥ 4 standard drinks per day or ≥ 28 drinks per week, (3) self-reported alcoholics or a history of alcoholism, (4) any attendance or inpatient stay for alcohol or drug treatment, (5) display any characteristic of current or future substance abuse disorders, (6) presence of any psychiatric, behavioral, or medical disorder that, in the opinion of the PIs, Co-Is, or MI, may interfere with study participation, the ability to adhere to the protocol, or has the potential for increased substance abuse, (7) prescription medications that interact with alcohol intake, (8) abnormal screening laboratory safety tests, (9) smokers, (10) diagnosis of Type 1 or 2 diabetes mellitus, cancer, or major organ disease, (11) serious digestive disorders, (12) conditions that affect metabolism or body weight (i.e., uncontrolled thyroid conditions, bariatric surgery, pregnancy, breastfeeding), (13) hysterectomy and/or hysterectomy with bilateral salphingo-oopherectomy, (14) hormonal pharmaceutical contraceptives including oral contraception (birth control pills), injectables (Depo-Provera), or the patch (Xulane), (15) Polycystic Ovary Syndrome (PCOS), and (16) use of medications that affect body weight or metabolism (i.e., atypical antipsychotics, weight loss medications).…”