Validation of Strasbourg environmental exposure chamber (EEC) ALYATEC<sup>®</sup> in mite allergic subjects with asthma

Khayath, N.; Doyen, Virginie; Gherasim, Alina; Radu, Carmen; Choual, Ibrahim; Beck, N.; Jacob, Audrey; Schoettel, Florian; Vecellio, Laurent; Domis, Nathalie; Blay, F. De

doi:10.1080/02770903.2018.1563902

Cited by 19 publications

(31 citation statements)

References 29 publications

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“…The volume of the EEC is 147 m 3, (area = 65 m 2 ) with a capacity up to 20 seats. During allergen exposures, airborne particles number and size are continuously monitored in order to ensure homogenous distribution of allergen 16 , 17 In this study, participants were exposed to the same batch of lyophilized birch allergen extract (100 IR; Stallergenes Greer ®), diluted in a NaCl solution, as the one used for the CAC. Before each EEC exposure, lyophilized birch allergen was prepared in a NaCl solution and nebulized in the EEC.…”

Section: Methodsmentioning

confidence: 99%

Birch allergen challenges in allergic conjunctivitis using standard conjunctival allergen challenge and environmental exposure chamber

Gherasim¹,

Fauquert²,

Domis³

et al. 2021

Clinical & Translational All

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Background Environmental exposure chambers (EECs) have been used extensively to study allergic rhinoconjunctivitis. Few studies have been published using EECs in conjunctivitis only, and none have used conjunctival allergen challenge as a selection criterion. The present study validated ALYATEC EEC in allergic conjunctivitis to birch pollen. Methods Sixteen patients with a positive conjunctival allergen challenge (CAC) were exposed to 60 ng/m 3 of Bet v 1 in an EEC on two consecutive days for a maximum of 4 h to validate EEC exposure to birch. Reproducibility was tested among seven of the patients. A conjunctival positive scoring during the CAC and the EEC exposure was defined as a Total Ocular Symptom Score (TOSS) ≥ 5. Results Fifty percent of patients had a conjunctival positive scoring during first exposure and 75% during second exposure. The mean time to a conjunctival response was 81.2 ± 33.9 min and 101.6 ± 57 ( P > 0.05) during first and second exposure, respectively. No difference in TOSS occurred between the two exposures. The time necessary to obtain a positive response during the CAC was significantly shorter than with the EEC. The estimated quantity of Bet v 1 inducing a positive response was 0.07 ± 0.03 ng (exposure 1), 0.07 ± 0.07 ng (exposure 2), 980 ± 784 ng (CAC). Conjunctival positive scoring and quantity of Bet v 1 was reproducible in all six EEC exposures. Conclusions Early conjunctival responses induced by birch allergen exposures in EEC were different than from those identified with direct instillation during CAC. EEC appears to be closer to natural exposure than CAC.

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Section: Methodsmentioning

confidence: 99%

Birch allergen challenges in allergic conjunctivitis using standard conjunctival allergen challenge and environmental exposure chamber

Gherasim¹,

Fauquert²,

Domis³

et al. 2021

Clinical & Translational All

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“…This study enroled 24 adults (13 women and 11 men) with a history of cat‐allergic asthma. The inclusion criteria were as follows: controlled intermittent asthma (step 1 treatment, only under short‐acting beta 2 agonist as needed, according to GINA Guideline 2016) with or without association with cat allergy‐related rhinoconjunctivitis; sensitization to cat allergen extract as defined by skin prick test for cat extract with a wheal diameter of ≥3 mm greater than negative control (ALK, HØrsholm, Denmark) and specific IgE anti‐Fel d 1 ≥ 0.7 kU/L (ImmunoCAP, Thermoficher), FEV1 ≥ 70% of the predicted value; no cat exposure at home or outside of the home during the study; positive methacholine challenge test performed using an AeroDoseur DTF‐Atomisor ATO‐AD 12 as previously published and early asthmatic response during baseline cat allergen exposure.…”

Section: Methodsmentioning

confidence: 99%

“…The EEC was conceived as a clean room, constructed with low‐emitting, non‐adherent, easily‐cleaned materials. All details concerning the airflow system, environmental conditions (endotoxins and VOCs levels), airborne particles and allergens have been published elsewhere . Patients were asked to wear protective coverall with integrated socks before entering the EEC and during the 2‐hours exposure in order to avoid contamination with outside allergens and with inside allergens when getting out of the EEC.…”

Section: Methodsmentioning

confidence: 99%

“…The nebulization was initiated before the subject entered the EEC, in order to reach the plateau of Fel d 1 aeroallergen. Then, a continuous nebulization was required after the subjects entered the EEC and lasted 2‐hours maximum during exposures …”

Section: Methodsmentioning

confidence: 99%

“…The ALYATEC EEC is a new‐generation chamber that provides standardized and controlled allergenic and non‐allergenic exposure, located in the Strasbourg University Hospital within 3 minutes of the intensive care unit. It has previously been used for validation studies with mite and cat allergen extracts in patients with asthma.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of air cleaners in asthmatics allergic to cat in ALYATEC^® environmental exposure chamber

Gherasim¹,

Jacob²,

Schoettel³

et al. 2019

Clin Experimental Allergy

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Background Air cleaners have been promoted for respiratory allergic disease prevention, but there is no clear clinical proof of their efficacy in allergic asthma. Objective To examine the efficacy of a new air cleaner on early and late asthmatic responses in cat‐allergic patients. Methods This randomized, cross‐over, double‐blind placebo‐controlled study enrolled 24 cat‐asthmatic patients with GINA 1 asthma. At baseline, participants were exposed to 40 ng/m3 of airborne cat allergen for a maximum of 2 hours in ALYATEC® environmental exposure chamber (EEC). All participants were subsequently randomized into two groups that were exposed to cat allergen, either with active then placebo air cleaners or with placebo then active air cleaners with a 3‐week interval in the EEC. This study was registered under number (NCT03928561). Results Fewer patients experienced an EAR with active air cleaners (seven patients; 29.17%) than placebo (21 patients; 87.50%). The response incidence was lower with active than with placebo air cleaners. A Cox model demonstrated a significant treatment effect (hazard ratio, 0.10; P = .002). Active air cleaners also prevented late asthmatic response: four patients (16.67%) had a late asthmatic response with active air cleaners compared to 11 patients (45.83%) with placebo (Prescott test P = .002). Active air cleaners also decreased the maximal severity of bronchial response (FEV1 decrease of 17.24% with active vs 25.62% with placebo air cleaners; P = .001). Conclusions Our present results demonstrated that Intense Pure Air XL® air cleaners significantly prevented early and late asthmatic responses among cat‐allergic asthmatics during cat allergen exposure in the ALYATEC® EEC.

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International consensus statement on allergy and rhinology: Allergic rhinitis – 2023

Wise

Damask

Roland

et al. 2023

Int Forum Allergy Rhinol

108

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Background In the 5 years that have passed since the publication of the 2018 International Consensus Statement on Allergy and Rhinology: Allergic Rhinitis (ICAR‐Allergic Rhinitis 2018), the literature has expanded substantially. The ICAR‐Allergic Rhinitis 2023 update presents 144 individual topics on allergic rhinitis (AR), expanded by over 40 topics from the 2018 document. Originally presented topics from 2018 have also been reviewed and updated. The executive summary highlights key evidence‐based findings and recommendation from the full document. Methods ICAR‐Allergic Rhinitis 2023 employed established evidence‐based review with recommendation (EBRR) methodology to individually evaluate each topic. Stepwise iterative peer review and consensus was performed for each topic. The final document was then collated and includes the results of this work. Results ICAR‐Allergic Rhinitis 2023 includes 10 major content areas and 144 individual topics related to AR. For a substantial proportion of topics included, an aggregate grade of evidence is presented, which is determined by collating the levels of evidence for each available study identified in the literature. For topics in which a diagnostic or therapeutic intervention is considered, a recommendation summary is presented, which considers the aggregate grade of evidence, benefit, harm, and cost. Conclusion The ICAR‐Allergic Rhinitis 2023 update provides a comprehensive evaluation of AR and the currently available evidence. It is this evidence that contributes to our current knowledge base and recommendations for patient evaluation and treatment.

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Validation of Strasbourg environmental exposure chamber (EEC) ALYATEC^® in mite allergic subjects with asthma

Cited by 19 publications

References 29 publications

Birch allergen challenges in allergic conjunctivitis using standard conjunctival allergen challenge and environmental exposure chamber

Birch allergen challenges in allergic conjunctivitis using standard conjunctival allergen challenge and environmental exposure chamber

Efficacy of air cleaners in asthmatics allergic to cat in ALYATEC^® environmental exposure chamber

International consensus statement on allergy and rhinology: Allergic rhinitis – 2023

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Validation of Strasbourg environmental exposure chamber (EEC) ALYATEC® in mite allergic subjects with asthma

Cited by 19 publications

References 29 publications

Birch allergen challenges in allergic conjunctivitis using standard conjunctival allergen challenge and environmental exposure chamber

Birch allergen challenges in allergic conjunctivitis using standard conjunctival allergen challenge and environmental exposure chamber

Efficacy of air cleaners in asthmatics allergic to cat in ALYATEC® environmental exposure chamber

International consensus statement on allergy and rhinology: Allergic rhinitis – 2023

Contact Info

Product

Resources

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Validation of Strasbourg environmental exposure chamber (EEC) ALYATEC^® in mite allergic subjects with asthma

Efficacy of air cleaners in asthmatics allergic to cat in ALYATEC^® environmental exposure chamber