Validation of standard method EN ISO 22964:2017 — Microbiology of the food chain — Horizontal method for the detection of Cronobacter spp.

Benito, Amparo; Besse, Nathalie Gnanou; Desforges, Isabelle; Gerten, Barbara; Ruíz, B.; Tomás, David

doi:10.1016/j.ijfoodmicro.2018.03.025

Cited by 16 publications

(4 citation statements)

References 12 publications

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“…The Panel noted that in a publication on an inter‐lab trial of the ISO 22964:2017 method (De Benito et al., 2018), the sensitivity value, expressed as LOD‐50 corresponding to the level of detection for which 50% of tests give a positive result was calculated for three food items (two powdered infant formulas and one starch) with values between 0.8 and 1.1 colony‐forming unit (CFU)/sample. This sensitivity is adequate.…”

Section: Assessmentmentioning

confidence: 99%

Opinion on the re‐evaluation of lecithins (E 322) as a food additive in foods for infants below 16 weeks of age and follow‐up of its re‐evaluation as food additive for uses in foods for all population groups

Flavourings¹,

Younes²,

Aquilina³

et al. 2020

EFS2

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Section: Assessmentmentioning

confidence: 99%

Opinion on the re‐evaluation of lecithins (E 322) as a food additive in foods for infants below 16 weeks of age and follow‐up of its re‐evaluation as food additive for uses in foods for all population groups

Flavourings¹,

Younes²,

Aquilina³

et al. 2020

EFS2

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“…4−6 To prevent the outbreak of C. sakazakii, it is desired to quickly screen all aspects of the food production chain, especially formula milk powder (PIF). 7 The standard method for C. sakazakii detection is a culturebased method (ISO 22964), 8,9 which typically takes up 3−4 days (Scheme 1A). The nucleic acid testing (NAT)-based method, 10−13 e.g., qPCR, features high sensitivity and specificity in C. sakazakii testing and can shorten the overall time to 1 day.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The standard method for C. sakazakii detection is a culture-based method (ISO 22964), , which typically takes up 3–4 days (Scheme A). The nucleic acid testing (NAT)-based method, − e.g., qPCR, features high sensitivity and specificity in C.…”

Section: Introductionmentioning

confidence: 99%

Integrating Recombinase Polymerase Amplification and Photosensitization Colorimetric Detection in One Tube for Fast Screening of C. sakazakii in Formula Milk Powder

Wang,

Zheng,

et al. 2024

Anal. Chem.

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Cronobacter sakazakii (C. sakazakii) is a widely existing opportunistic pathogen and thus threatens people with low immunity, especially infants. To prevent the outbreak, a rapid and accurate on-site testing method is required. The current standard culture-based method is time-consuming (3−4 days), while the nucleic acid amplification (PCR)based detection is mostly carried out in central laboratories. Herein, isothermal recombinase polymerase amplification (RPA) coupled with a photosensitization colorimetric assay (PCA) was adopted for the on-site detection of C. sakazakii in powdered infant formulas (PIFs). The lowest visual detection concentration of C. sakazakii is 800 cfu/mL and 2 cfu/g after 8 h bacteria pre-enrichment. Furthermore, to avoid typical cap opening-resulted aerosol pollution, the PCA reagents were lyophilized onto the cap of the RPA tube (containing lyophilized RPA reagents). After amplification, the tube was subjected to simple shaking to mix the PCA reagents with the amplification products for lightdriven color development. Such a one-tube assay offered a lowest concentration of 1000 copies of genomic DNA of C. sakazakii within 1 h. After 8 h of bacterial enrichment, the lowest detecting concentration could be pushed down to 5 cfu/g bacteria in PIF. To facilitate on-site monitoring, a portable, battery-powered PCA device was designed to mount the typical RPA 8-tube strip, and a color analysis cellphone APP was further employed for facile readout.

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“…Cronobacter sakazakii has been detected using traditional selective culture methods, biochemical reactions, and PCR technology (Minami et al, 2012;ISO, 2017;de Benito et al, 2019;Akineden et al, 2020), but these conventional methods are laborious and timeconsuming. Indeed, PCR-based methods require total DNA extraction and target DNA amplification, which may result in false positives (Friedemann, 2007), and they require several hours for detection (Srikumar et al, 2019;Yuan et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive detection of Cronobacter sakazakii based on immunochromatography coupled with surface-enhanced Raman scattering

Gao

Wu²,

Wang

et al. 2021

Journal of Dairy Science

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The presence of Cronobacter sakazakii must be controlled in infant powder plants, because it may cause infectious disease in infants, with high mortality. Testing for C. sakazakii in powdered infant formula should be performed before delivery, and it requires rapid and specific detection methods. In this study, we established a surface-enhanced Raman scattering (SERS) immunochromatographic test strip for the quantitative determination of C. sakazakii in powdered infant formula. Monoclonal antibodies for C. sakazakii were labeled with p-aminothiophenol-bound colloidal gold nanoparticles. Color change in the test line indicated the presence of C. sakazakii. A highly sensitive and quantitative test method was developed based on the Raman signal produced by the p-aminothiophenol bonding on gold nanoparticles. The SERS immunochromatographic test strip assay required a short analysis time (12 min) and exhibited a linearity range from 10 2 to 10 7 cfu/mL. The limit of detection was 201 cfu/mL without preculture. The SERS immunochromatographic test strip assay is a promising tool for the simple and rapid quantitative analysis of C. sakazakii and other pathogenic bacteria.

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Validation of standard method EN ISO 22964:2017 — Microbiology of the food chain — Horizontal method for the detection of Cronobacter spp.

Cited by 16 publications

References 12 publications

Opinion on the re‐evaluation of lecithins (E 322) as a food additive in foods for infants below 16 weeks of age and follow‐up of its re‐evaluation as food additive for uses in foods for all population groups

Opinion on the re‐evaluation of lecithins (E 322) as a food additive in foods for infants below 16 weeks of age and follow‐up of its re‐evaluation as food additive for uses in foods for all population groups

Integrating Recombinase Polymerase Amplification and Photosensitization Colorimetric Detection in One Tube for Fast Screening of C. sakazakii in Formula Milk Powder

Highly sensitive detection of Cronobacter sakazakii based on immunochromatography coupled with surface-enhanced Raman scattering

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