“…The design/identification of biochemical pathways to convert gases to MEG is non-trivial (Medema et al, 2012) and can be tackled with the help of state-of-the-art cheminformatics and metabolic engineering tools, such as BNICE.ch (Hadadi et al, 2016;Hatzimanikatis et al, 2005), DESHARKY (Rodrigo et al, 2008), GEM-path (Campodonico et al, 2014), RetroPath (Fehér et al, 2014), and XTMS . Almost all of these cheminformatics tools are based on the retrobiosynthesis principle (Carbonell et al, 2013;Hadadi and Hatzimanikatis, 2015;Medema et al, 2012), in which a set of defined biotransformation rules are iteratively applied to connect a target compound "retrosynthetically" to the metabolites of either a host organism's metabolic network, or a biochemical database such as KEGG (Kanehisa et al, 2016), MetaCyc (Caspi et al, 2014), and PubChem (Kim et al, 2016).…”