Validation of QTLs for Fiber Quality Introgressed from Gossypium mustelinum by Selective Genotyping

Abstract

<jats:p>Gene introgression from wild species has been shown to be a feasible approach for fiber quality improvement in Upland cotton. Previously, we developed an interspecific <jats:italic>G. mustelinum</jats:italic> × <jats:italic>G. hirsutum</jats:italic> advanced-backcross population and mapped over one hundred QTL for fiber quality traits. In the current study, a trait-based selective genotyping approach was utilized to prioritize a small subset of introgression lines with high phenotypic values for different fiber quality traits, to simultaneously validate multiple fiber quality QTL in a single experiment. A total of 75 QTL were detected by CIM and/or single-marker analysis, including 11 significant marker-trait associations (<jats:italic>P</jats:italic> &lt; 0.001) and three putative associations (<jats:italic>P</jats:italic> &lt; 0.005) also reported in earlier studies. The QTL that have been validated include three each for fiber length, micronaire, and elongation, and one each for fiber strength and uniformity. Collectively, about 10% of the QTL previously reported have been validated here, indicating that selective genotyping has the potential to validate multiple marker-trait associations for different traits, especially those with a moderate to large-effect detected simultaneously in one experimental population. The <jats:italic>G. mustelinum</jats:italic> alleles contributed to improved fiber quality for all validated loci. The results from this study will lay the foundation for further fine mapping, marker-assisted selection and map-based gene cloning.</jats:p>

Analyzing Quantitative Trait Loci for Fiber Quality and Yield-Related Traits From a Recombinant Inbred Line Population With Gossypium hirsutum Race palmeri as One Parent

“…By comparing the physical confidence interval of QTLs in different studies, 35 stable QTLs in this work were found sharing overlapped interval with previous reported QTL (Supplementary Table 11), and 15 of them were detected in more than one previous work. Among them, qFL_A10.3 was found to be the same with QTLs in other five works (Liang et al, 2013;Shao et al, 2014;Wang H. et al, 2016;Shi et al, 2019), and qFL_D11.1 and qLP_D03.1 were found to be the same with QTLs in other four works, respectively (Wang et al, , 2020Ning et al, 2014;Shi et al, 2015;Jamshed et al, 2016;Diouf et al, 2018;Ma J. et al, 2018). Six QTLs (qFE_D02.3, qFL_A08.5, qFL_D07.1, qFL_D13.3, qFS_D01.1, qFS_D07.1) were identified in other three previous works (Shen et al, 2006;Feng et al, 2015;Tang et al, 2015;Wang F. et al, 2016;Zhang et al, 2016).…”

“…A total of 72 stable QTLs were identified, and many of them had been reported in previous studies ( Shen et al, 2005 , 2019 ; Zhang et al, 2011 ; Su et al, 2013 ; Yu et al, 2013 ; Shang et al, 2015 , 2016 ; Guo et al, 2018 ; Deng et al, 2019 ; Ma et al, 2019 ; Chen et al, 2020 ). By comparing the physical confidence interval of QTLs in different studies, 35 stable QTLs in this work were found sharing overlapped interval with previous reported QTL ( Supplementary Table 11 ), and 15 of them were detected in more than one previous work.…”

Genomic and Cytogenetic Analysis of Synthetic Polyploids between Diploid and Tetraploid Cotton (Gossypium) Species

“…The historical domestication and modern breeding of Upland cotton ( Gossypium hirsutum ) have significantly improved its yield and fiber quality, but this has led to a dramatically reduced genetic diversity [ 24 , 25 , 26 , 27 ]. Upland cotton cultivars have a narrow genetic base of fiber quality and abiotic stress tolerance traits.…”

“…The fiber of G. mustelinum is genetically different from that of allotetraploid G. hirsutum . The quantitative trait loci (QTL) for fiber quality and the interesting alleles identified from G. mustelinum can make an important contribution to the improvement of Upland cotton germplasm [ 25 ]. Wang et al [ 30 ] dissected the molecular genetic basis of fiber strength and fineness in G. mustelinum and G. hirsutum crosses.…”

“…The study laid a foundation for cotton genetics and breeding, emphasizing the rich gene pool of G. mustelinum . Chen et al [ 25 ] used selective genotyping to validate over 75 QTLs for fiber quality traits that were introgressed from G. mustelinum into Upland cotton. The study lays the foundation for fine mapping, marker-assisted selection, and map-based gene cloning.…”