Validation of PM2.5 model particle through physicochemical evaluation and atherosclerotic plaque formation in ApoE-/- mice

Progress in research on effect of PM2.5 on occurrence and development of atherosclerosis

“…Moreover, simulated PM 2.5 exhibited similar cytotoxic and detrimental effects on lipid metabolism and atherosclerotic plaque formation as actual PM 2.5 . Hence, this model particle was prominent in the evaluation of the effect of specific organic compositions bound to PM 2.5 and the concentration on human health (Zhao et al, 2020).…”

Lipid Dysregulation Induced by Gasoline and Diesel Exhaust Exposure and the Interaction with Age

“…The liquid containing PM 2.5 was filtered through six layers of sterile gauze and centrifuged at 12,000 rpm at 4 °C for 30 min. Detached PM 2.5 was then vacuum-freeze dried, and the mass was weighed [ 34 ]. PM 2.5 was resuspended in a certain amount of sterile saline to achieve PM 2.5 suspensions and stored at −20 °C until further experimentation.…”

Shenlian Extract in the Treatment of Ultrafine Particulate Matter- Aggravated Myocardial Ischemic Injury: Integrating Network Pharmacology and in vivo Pharmacological Evaluation

“…Prior research indicated that long-term exposure to particulate matter can result in newly formed plaques, which can develop into fibrous fatty plaques that are prone to breakage and instability [10]. This mechanism has also been demonstrated in animal models, in which exposure to higher levels of particulate matter potentiated atherosclerosis by increasing the generation of reactive oxygen species, inducing vascular inflammation, and promoting lipid metabolism disorder and the instability of plaques [11,12]. In addition, epidemiological research illustrated that the severity of coronary artery disease on invasive coronary angiography increases with higher long-term exposure to particulate matter [13].…”