Validation of multiplex PCR strategy for simultaneous detection and identification of methicillin resistant<i>Staphylococcus aureus</i>

Rallapalli, Suneel; Verghese, S; Verma, RS

doi:10.4103/0255-0857.43580

Cited by 25 publications

(6 citation statements)

References 10 publications

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“…The varied ethnobotanical uses of S. chirayita have led to the initiation of various pharmacological investigations. Previous research demonstrates that the S. chirayita extracts exhibit a wide range of biological activities, such as antibacterial, antifungal, antiviral, anticancer, anti-inflammatory, and others like antidiabetic and antioxidant activities (Verma et al, 2008 ; Alam et al, 2009 ; Arya et al, 2011 ; Chen et al, 2011 ; Laxmi et al, 2011 ). Concurrently, a diverse range of in vitro and in vivo test systems has been used to evaluate the pharmacological properties of S. chirayita .…”

Section: Pharmacological Activitymentioning

confidence: 99%

A Review of Swertia chirayita (Gentianaceae) as a Traditional Medicinal Plant

2016

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Swertia chirayita (Gentianaceae), a popular medicinal herb indigenous to the temperate Himalayas is used in traditional medicine to treat numerous ailments such as liver disorders, malaria, and diabetes and are reported to have a wide spectrum of pharmacological properties. Its medicinal usage is well-documented in Indian pharmaceutical codex, the British, and the American pharmacopeias and in different traditional medicine such as the Ayurveda, Unani, Siddha, and other conventional medical systems. This ethnomedicinal herb is known mostly for its bitter taste caused by the presence of different bioactive compounds that are directly associated with human health welfare. The increasing high usage of Swertia chirayita, mostly the underground tissues, as well as the illegal overharvesting combined with habitat destruction resulted in a drastic reduction of its populations and has brought this plant to the verge of extinction. The increasing national and international demand for Swertia chirayita has led to unscrupulous collection from the wild and adulteration of supplies. The aim of this review is to provide a synthesis of the current state of scientific knowledge on the medicinal uses, phytochemistry, pharmacological activities, safety evaluation as well as the potential role of plant biotechnology in the conservation of Swertia chirayita and to highlight its future prospects. Pharmacological data reported in literature suggest that Swertia chirayita shows a beneficial effect in the treatment of several ailments. However, there is lack of adequate information on the safety evaluation of the plant. The pharmacological usefulness of Swertia chirayita requires the need for conservation-friendly approaches in its utilization. Providing high-quality genetically uniform clones for sustainable use and thereby saving the genetic diversity of this species in nature is important. In this regard, plant biotechnological applications such as micropropagation, synthetic seed production, and hairy root technology can play a significant role in a holistic conservation strategy. In addition to micropropagation, storage of these valuable genetic resources is equally important for germplasm preservation. However, more advanced research is warranted to determine the activities of bioactive compounds in vitro and in vivo, establish their underlying mechanisms of action and commence the process of clinical research.

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Section: Pharmacological Activitymentioning

confidence: 99%

A Review of Swertia chirayita (Gentianaceae) as a Traditional Medicinal Plant

2016

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“…MLST, on the other hand, is highly discriminatory, but is low throughput and expensive [ 34 , 45 ]. Multiplex PCR is a good compromise method, which offers the advantages of rapidly and relatively easily obtainable results, concordance with phenotypic tests and compatibility with patient management [ 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Based Phenotype and Hospital Admission Profile are the Most Likely Predictors of Genotyping Classification of MRSA

et al. 2017

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Background:Methicillin-resistant Staphylococcus aureus (MRSA) infection is associated with increased morbidity, mortality, and financial burdens. Phenotyping methods are used to classify MRSA as either health care MRSA (HA-MRSA) or community-associated MRSA (CA-MRSA). Recent studies suggested the phenotyping methods are not always reliable, based on a lack of concordance with genotyping results.Objective:In this study, concordance of classification methods based on clinical characteristics or antibiotic susceptibility compared to the gold standard genotyping was assessed in the classification of MRSA.Methods:We compared the genotypes and phenotypes of MRSA in 133 samples taken from patients in Saudi Arabia. Statistical analyses included concordance, specificity and sensitivity, and logistic regression modeling.Results:There was fair a definite agreement between the health care risk and infection type methods (p < .001), but no statistically significant agreement between the susceptibility pattern and health care risk methods (p = 243), and between susceptibility pattern and infection type methods (p = .919). Reduced multiple regression modelling suggested the potential of a phenotyping-based method of antibiotic susceptibility pattern (OR = 15.47, p < .001) in conjunction with hospital admission profile(OR = 2.87, p = .008) to accurately identify MRSA as HA-MRSA and CA-MRSA.Conclusion:The use of a standardized phenotyping technique, using susceptibility pattern and hospital admission profiles to classify MRSA infections as either HA-MRSA or CA-MRSA, would facilitate diagnosis, infection control efforts, prevention, and assignment of appropriate therapies. The ability to use phenotyping in the classification of these strains would improve efforts to contend with this adept and evolving bacterial organism.

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“…[32] Another Indian medicinal plant extract Swertia chirata inhibited HSV-1 viral dissemination. [33] Putranjivain A, isolate of Euphorbia jolkini Bioss, inhibited both the virus entry and late stage replication of HSV-2 in-vitro . [13] Kenyan plant Carissa edulis aqueous extract exhibited efficacy against both in-vitro and in-vivo models of HSV-1 infection.…”

Section: Discussionmentioning

confidence: 99%