Validation of molecular markers linked to apospory in tetraploid races of bahiagrass, Paspalum notatum Flüggé

Rebozzio, Romina Natalia; Rodríguez, María Pía; Stein, Juliana; Ortiz, Juan Pablo Amelio; Quarín, Camilo Luis; Espinoza, Francisco

doi:10.1007/s11032-010-9537-7

Cited by 11 publications

(10 citation statements)

References 33 publications

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“…However, the data shown by Fortes et al (2004), after analyzing 44 hybrids using RAPD markers, showed similar results to the cytoembryological analysis, finding more apomictic than sexual plants. Rebozzio et al (2012), using RAPD markers BCU 243 and BCU 259 along with eleven AFLP markers, showed that these markers, previously connected to the aposporic region in the apomictic plant Q4117, were also conserved in other apomictic accessions, although the marker of the BCU 243 primer in particular presented a band clearly linked to the aposporic region. According to the same authors, in an attempt to obtain SCAR (sequencecharacterized amplified regions) markers, which would be more specific in the identification of apomictic plants, it was only possible to develop a SCAR marker from an AFLP (amplified fragment length polymorphism) marker (Espinoza et al, 2006).…”

Section: Resultsmentioning

confidence: 95%

Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers

et al. 2017

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-The aim of this study was to determine the mode of reproduction of a hybrid progeny derived from intraspecific crosses of Paspalum notatum through cytoembryological analysis and use of RAPD (random amplification of polymorphic DNA) molecular markers. Cytoembryological analysis allowed identification of the mode of reproduction of 28 plants that were selected after agronomic productivity evaluations. Of these, 19 had embryo sac morphology compatible with an apomictic mode of reproduction and nine had embryo sac morphology compatible with a sexual mode of reproduction. Meanwhile, molecular marker analysis for 194 individuals showed 54 sexual and 140 apomictic plants; of the 28 plants analyzed by the two methods, ten results (35.7%) were in disagreement. In this paper, through cytoembryological analyses, a ratio of 1:2.1 of sexual to apomictic plants was found. The BCU 243 marker showed a stable pattern of amplification, but some results differed with cytoembryological analyses, demonstrating that these analyses are more reliable when determining the mode of sexual reproduction for the plants of P. notatum. Apomictic plants characterized in this work can be tested in the field to check their agronomic value and registration as plant varieties, while the sexual plants can be used as potential parents in future crosses.

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Section: Resultsmentioning

confidence: 95%

Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers

et al. 2017

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“…Completely sexual tetraploid plants were produced directly from sexual diploids plants of natural bahiagrass populations by colchicine treatment (Quarin evaluation of segregating progeny Weiler et al, 2015). The subsequent use of duplicate genotypes in intraspecific mating systems opens new possibilities for breeding programs (Rebozzio et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Intraspecific tetraploid hybrids of Paspalum notatum: agronomic evaluation of segregating progeny

Weiler

Dall'Agnol

Simioni

et al. 2018

Sci. agric. (Piracicaba, Braz.)

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For many decades, animal production in southern Brazil has been based on native forage grasses. Paspalum notatum Flügge (bahiagrass) is one of the most frequently used native grass in southern Brazil. The native germplasm of P. notatum is tetraploid and displays apomictic reproduction; hence, chromosome doubling of sexual diploid accessions is required to facilitate hybridization. The main goal of this study was to evaluate forage production and other agronomical evaluation of P. notatum intraspecific hybrids in order to obtain new varieties that could be registered and/or protected and launched as new cultivars in the future. Three tetraploid sexual genotypes were crossed with the ecotypes Bagual and André da Rocha. Biomass production (leaf, stem, and inflorescence fractions), plant growth habit, plant height, number of tillers, and frost damage were evaluated in the resulting hybrids. The general combining ability (GCA) and specific combining ability (SCA) were calculated for the six families generated. We evaluated 196 hybrids and the most productive yielded 42 % more total dry mass than the most productive parent (ecotype Bagual), and Bagual production was seven-fold higher than that of cultivar 'Pensacola'. The highest GCA values were observed for Bagual and Q4205. No correlation was observed between leaf coloration and the frost effect or regrowth in the following winter.Similarly, no correlation between plant growth habit and dry mass production was observed. The reproduction mode of the most productive hybrids are currently being analyzed in larger plots to identify apomictic and sexual genotypes for future registration and protection.

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“…A group of RAPD, RFLP and AFLP molecular markers linked to the apospory locus has been identified (Martínez et al , 2003; Pupilli et al , 2004; Stein et al , 2004, 2007; Rebozzio et al , 2012). These markers define a chromosome block characterized by recombination restriction and preferential chromosome pairing (Martínez et al , 2003; Stein et al , 2004).…”

Section: Introductionmentioning

confidence: 99%

Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum

et al. 2012

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In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen molecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2–4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence mapping in silico in the apospory-syntenic regions of rice and maize. Approximately 5 kb corresponding to different markers were characterized through the global sequencing procedure. A more refined analysis based on sequence information indicated synteny with segments of chromosomes 2 and 12 of rice and chromosomes 3 and 5 of maize. Two loci associated with apomixis locus were tested in methylation-sensitive RFLP experiments using genomic DNA extracted from leaves. Although both target sequences were methylated no methylation polymorphisms associated with the mode of reproduction were detected.

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Validation of molecular markers linked to apospory in tetraploid races of bahiagrass, Paspalum notatum Flüggé

Cited by 11 publications

References 33 publications

Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers

Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers

Intraspecific tetraploid hybrids of Paspalum notatum: agronomic evaluation of segregating progeny

Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum

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