Validation of molecular markers linked to fertility restorer gene(s) for WA-CMS lines of rice

Sheeba, NK; Viraktamath, B. C.; Sivaramakrishnan, S.; Gangashetti, M. G.; Khera, Pawan; Sundaram, R. M.

doi:10.1007/s10681-008-9865-4

Cited by 52 publications

(42 citation statements)

References 16 publications

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“…The hybrids produced using converted Swarna breeding lines with Rf3 gene showed 15-20% of more pollen and spikelet fertility than the partial restorer Swarna. This clearly demonstrates that that Rf3 gene may be contributing 15 to 30% for the fertility restoration trait of WA-CMS and Rf4 may be contributing about 70% for the same trait and presence of both the genes can bestow complete fertility restoration 13,[33][34][35][36][37][38] .…”

Section: Discussionmentioning

confidence: 99%

Conversion of partial restorer Swarna into restorer by transferring fertility restorer Rf gene(s) through marker assisted back cross breeding (MABB) in rice

Ponnuswamy

Singh

Raman

et al. 2020

Sci Rep

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the major constraints in hybrid rice breeding are availability of limited number of parental lines with specific desirable traits and lower frequency of restorers among elite breeding lines. The popular, high-yielding mega-rice variety Swarna, has been identified to be a partial restorer (as it has only one of major fertility restorer genes, Rf4) and hence cannot be utilized directly in the hybrid rice breeding. To convert the partial restorer to complete restorer, a cross was made between Swarna and a stable restorer KMR3R possessing Rf3 and Rf4 genes and developed BC 1 f 5 and Bc 2 f 4 populations by markerassisted back cross breeding (MABB). The SSR marker DRRM-RF3-10 linked to Rf3 gene located on chromosome 1, clearly distinguished restorers from partial restorers. All the improved lines of Swarna possessing Rf3 and Rf4 genes showed complete fertility restoration in test crosses with higher grain yield heterosis. few rice hybrids developed by using converted restorers were evaluated in multi location testing under the All india co-ordinated Rice improvement project (AicRip). the results indicated that new rice hybrids expressed higher heterosis with matching grain quality attributes like Swarna. This study provides significantly novel and relevant restorers to enhance and economize future hybrid rice breeding programs.Rice (O. sativa L.) is the most important staple food crop for more than half of the world's population and it is cultivated in an area of 44.5 million hectare in India with the production of 106.5 million tonnes during the year 2016 1 . After the advent of high yielding semi-dwarf rice varieties, hybrid rice technology has been touted as a major strategy for enhancing the genetic yield potential of rice. The success of hybrid rice technology has been very well demonstrated in China, which produces 146.5 million tonnes of rice from 30.32 million hectares 1 . This significant increase in production in China is mainly due to cultivation of hybrid rice (with >50% area and production under rice hybrids). Several technical challenges, market and policy constraints has limited the development and diffusion of hybrid rice outside China 2 . In India, hybrid rice is cultivated in an area of ~3 million hectares 3 , which is about 6.7% of total area of rice cultivation. Hybrid rice accounts for less than 10% of the area under rice cultivation in Bangladesh, Indonesia, and the Philippines and just 10% in Vietnam.Hybrid rice technology aims to increase the yield potential of rice by exploiting the phenomenon of hybrid vigour or heterosis. Cytoplasmic male sterility coupled with fertility restoration controlled by nuclear genes is a very useful tool in exploiting heterosis in self pollinated crops. In rice, three CMS systems viz. Wild Abortive (WA), Boro II (BT) and Honglian (HL) are deployed for commercial hybrid rice seed production 4,5 . The most widely used CMS system in rice is based on wild abortive (WA) cytoplasm derived from Oryza sativa f. Spontanea 6,7 . The WA-CMS system is highly stable with compl...

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Section: Discussionmentioning

confidence: 99%

Conversion of partial restorer Swarna into restorer by transferring fertility restorer Rf gene(s) through marker assisted back cross breeding (MABB) in rice

Ponnuswamy

Singh

Raman

et al. 2020

Sci Rep

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“…Nas et al (2003) demonstrated the use of molecular markers for identification of restorer line. The usefulness of RM6100 in marker aided selection of restorer with selection accuracy of 97% and 94.87% was reported by Singh et al (2005) and Sheeba et al (2009) respectively. Revathi et al (2013) indicated that molecular screening for fertility restoration can be a useful tool for identifying restorers from breeding lines of unknown restoration status with 80 to 85% efficiency without making and evaluating large number of tedious test crosses.…”

Section: Discussionmentioning

confidence: 79%

Identification of fertility restorers among Assam rice cultivars by phenotyping and molecular screening approaches

Singh¹,

Borah²,

Ponnuswamy³

et al. 2016

Ind. Jrnl. Gen. Plnt. Bree.

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In three line hybrid rice seed production the combination of a CMS line, maintainer line and restorer line are involved in the development of hybrids. The wild abortive CMS is extensively used in commercial hybrid seed production. The fertility restoration of wild abortive CMS is governed by two independent dominant Rf genes viz., Rf4 and Rf3. The present study was conducted with the objective of molecular characterization rice genotypes from Assam, India and to identify restorers for hybrid rice breeding programme. A total one hundred and eleven hybrids were generated and evaluated for their pollen and spikelet fertility. Based on molecular screening twenty five genotypes were identified to carry only Rf4 gene and three genotypes identified to carry both Rf3 and Rf4 genes. These identified restorers in the present study would be a usable restores for hybrid rice breeding in north east India. Molecular diversity for fertility restoration using NTSYS package formed two clusters among the tested genotypes, cluster A represented a landrace Terabali from Assam and cluster B with all other genotypes.

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“…Of several rice microsatellite and EST-based STS markers used, the RM6100 marker was found to be tightly linked to the flanking Rf4 gene. Sheeba et al (2009) also validated its tight linkage with the WA-CMS fertility restorer in two mapping populations, and this marker was located at a distance of ~1.2 cM. However, molecular screening Table 2 Summary of the genotyping assay and accuracy predicted at Rf4 locus with both of A/C single nucleotide polymorphism (SNP)-based kompetitive allele specific PCR (KASP) and simple-sequence repeats (SSR) markers of RM6100…”

Section: Discussionmentioning

confidence: 99%

Significant association of the novel Rf4-targeted SNP marker with the restorer for WA-CMS in different rice backgrounds and its utilization in molecular screening

Chen

Yan

Dai

et al. 2017

Journal of Integrative Agriculture

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In the rice cytoplasmic-genetic male sterility (CMS) system, the combination of a CMS line, maintainer line and restorer line carrying the restorer gene to restore fertility, is indispensable for the development of hybrids. However, the process of screening for the trait of fertility restoration is laborious and time consuming. In the present study, we analyzed the nucleotide sequence of the Rf4 gene, which is the major locus controlling fertility restoration, to identify allele-specific variation. A SNP A/C at +474 in the CDS was found to be capable of strictly distinguishing groups of alleles Rf4 (A) and rf4 (C). Using KASP genotyping, this valuable SNP was converted to an allele-specific PCR marker. We evaluated and validated the marker among three-line parents with different backgrounds, and the results revealed a complete correlation between SNP alleles and the fertility restoration phenotype. Molecular screening was subsequently carried out for the presence of alleles of Rf4 and Rf3 among 328 diverse rice cultivars with worldwide distribution. The results demonstrate that this SNP marker could be the optimal choice for the molecular identification of potential restorers.

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Validation of molecular markers linked to fertility restorer gene(s) for WA-CMS lines of rice

Abstract: The present study was carried out with the objective to validate the molecular markers, which have been previously reported to be linked to fertility restorer (Rf) gene(s) for WA-CMS lines of rice. Two mapping populations involving fertility restorer lines for WA-cytoplasm, viz.

Cited by 52 publications

References 16 publications

Conversion of partial restorer Swarna into restorer by transferring fertility restorer Rf gene(s) through marker assisted back cross breeding (MABB) in rice

Conversion of partial restorer Swarna into restorer by transferring fertility restorer Rf gene(s) through marker assisted back cross breeding (MABB) in rice

Identification of fertility restorers among Assam rice cultivars by phenotyping and molecular screening approaches

Significant association of the novel Rf4-targeted SNP marker with the restorer for WA-CMS in different rice backgrounds and its utilization in molecular screening

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