Validation of micrografting to evaluate drought tolerance in micrografts of kiwifruits (Actinidia spp.)

Bao, Wen-Wu; Zhang, Xiaochen; Zhang, A-Ling; Zhao, Lei; Liu, Zhande

doi:10.1007/s11240-019-01727-y

Cited by 8 publications

(2 citation statements)

References 44 publications

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“…Plants also develop several antioxidative system which reduce the negative effect of ROS accumulation by detoxify them into less toxic compounds [ 38 , 39 ]. To eliminate and degrade harmful oxygen species at early stage, SOD and CAT are considered as most active enzymes that maintain oxidative equilibrium during stress condition [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Wild grapevines as rootstock regulate the oxidative defense system of in vitro grafted scion varieties under drought stress

et al. 2022

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The narrow genetic base of modern cultivars is becoming a key bottleneck for crop improvement and the use of wild relatives is an appropriate approach to improve the genetic diversity of crops to manage the sustainable production under different abiotic and biotic constraints. In Pakistan, wild germplasm of grapevine viz Dakh, Toran, and Zarishk belong to Vitis vinifera subsp. sylvestris and Fatati belong to Vitis vinifera subsp. sativa is naturally present in humid and sub-humid areas of mountainous and sub-mountainous regions and showed varying level of tolerance against drought stress but have not been evaluated as rootstock. In this study, different tolerant behavior of wild grapevines as rootstock in grafted scion varieties were explored under different levels of PEG-6000 mediated drought stress i.e., -4.00, -6.00, and -8.00 bars. In response to drought stress, wild grapevines evoked several non-enzymatic and enzymatic activities. Among non-enzymatic activities, total chlorophyll contents of commercial varieties were sustained at higher level when grafted on wild grapevines Dakh and Fatati which subsequently reduced the damage of cell membrane via MDA. Whereas, to cope the membranous damage due to excessive cellular generation of ROS, wild grapevines triggered the enhanced activities of SOD to dismutase the free oxygen radicals into H2O2, then CAT enzyme convert the H2O2 into water molecules. Higher accumulation of ROS in commercial scion varieties were also coped by wild grapevines Dakh and Fatati through the upregulation of POD and APX enzymes activities. Based on these enzymatic and non-enzymatic indices, biplot and cluster analysis classified the wild grapevines as rootstock into three distinct categories comprises on relatively tolerant i.e., Dakh (Vitis vinifera subsp. sylvestris) and Fatati (Vitis vinifera subsp. sativa), moderate tolerant i.e., Toran (Vitis vinifera subsp. sylvestris) and relatively susceptible category i.e., Zarishk (Vitis vinifera subsp. sylvestris).

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Section: Discussionmentioning

confidence: 99%

Wild grapevines as rootstock regulate the oxidative defense system of in vitro grafted scion varieties under drought stress

et al. 2022

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“…A partial pyridine nucleotide binding site is a conserved domain located in the active center of the lycopene cyclase enzymes [23,63,64]. The signal peptide in LCYB is found in the first 50-100 amino acids [54,65], and a conserved motif called the "conserved β-cyclase region" is found within the signal peptide that is proposed as a main factor for membrane association [54] and as an essential requirement for a correct catalytic activity [27].…”

Section: Carrot Dclcyb2 Encodes a Functional Lcyb Enzymementioning

confidence: 99%

Putative Daucus carota Capsanthin-Capsorubin Synthase (DcCCS) Possesses Lycopene β-Cyclase Activity, Boosts Carotenoid Levels, and Increases Salt Tolerance in Heterologous Plants

Rosas-Saavedra,

Quiroz,

Parra

et al. 2023

Plants

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Plant carotenoids are synthesized and accumulated in plastids through a highly regulated pathway. Lycopene β-cyclase (LCYB) is a key enzyme involved directly in the synthesis of α-carotene and β-carotene through the cyclization of trans-lycopene. Daucus carota harbors two LCYB genes, of which DcLCYB2 (annotated as CCS-Like) is mostly expressed in mature storage roots, an organ that accumulates high α-carotene and β-carotene content. In this work, we determined that DcLCYB2 of the orange Nantes variety presents plastid localization and encodes for a functional LCYB enzyme determined by means of heterologous complementation in Escherichia coli. Also, ectopic expression of DcLCYB2 in tobacco (Nicotiana tabacum) and kiwi (Actinidia deliciosa) plants increases total carotenoid content showing its functional role in plants. In addition, transgenic tobacco T2 homozygous plants showed better performance under chronic salt treatment, while kiwi transgenic calli also presented a higher survival rate under salt treatments than control calli. Our results allow us to propose DcLCYB2 as a prime candidate to engineer carotenoid biofortified crops as well as crops resilient to saline environments.

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Recent advances in argan propagation: a review

Mazri¹,

Koufan²,

Moussafir

et al. 2022

Trees

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Validation of micrografting to evaluate drought tolerance in micrografts of kiwifruits (Actinidia spp.)

Cited by 8 publications

References 44 publications

Wild grapevines as rootstock regulate the oxidative defense system of in vitro grafted scion varieties under drought stress

Wild grapevines as rootstock regulate the oxidative defense system of in vitro grafted scion varieties under drought stress

Putative Daucus carota Capsanthin-Capsorubin Synthase (DcCCS) Possesses Lycopene β-Cyclase Activity, Boosts Carotenoid Levels, and Increases Salt Tolerance in Heterologous Plants

Recent advances in argan propagation: a review

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