Validation of microarray comparative genomic hybridization for comprehensive chromosome analysis of embryos

Gutiérrez-Mateo, Cristina; Colls, P.; Sánchez-García, Jorge; Escudero, T.; Prates, R.; Ketterson, K.; Wells, Dagan; Munné, Santiago

doi:10.1016/j.fertnstert.2010.09.010

Cited by 287 publications

(218 citation statements)

References 38 publications

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“…A complete overview of embryonic chromosomes is difficult because of the number of in vitro cultured embryonic cells is generally small. However, recent technical developments in single cell amplification and microarray analysis make it possible to test chromosomes during the early embryonic stages [12][13][14].…”

Section: Discussionmentioning

confidence: 99%

Chromosomal characteristics at cleavage and blastocyst stages from the same embryos

Huang

Zhao

Wang

et al. 2015

J Assist Reprod Genet

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Purpose To investigate chromosomal characteristics in the same embryos at cleavage and blastocyst stage. Methods Six PGD/PGS cycles were retrospective studied, including five chromosomal translocation PGD cycles and one recurrent abortion PGS cycle. The cleavage embryos were biopsied one blastomere at day 3, followed by blastocyst culture. Trophectoderm cell biopsy was performed when embryos developed into the blastocyst stage. Whole genome amplification and 24-chromosomal analysis by CGH/SNP microarray was performed on each blastomere and trophectoderm cells. Results After PGD/PGS, only one couple had no euploid embryos to transfer. Five couples delivered a healthy, balancedkaryotype baby. A total of 18 embryos had both blastomere and trophectoderm cell reliable results available. Of the 18 embryos, eleven embryos contained identical chromosomes from cleavage stage to blastocyst stage and six embryos contained almost identical chromosomes . Only one embryo presented opposite chromosomal results for the cleavage and blastocyst stage, with abnormal chromosomes in the blastomere but normal chromosomes in trophectoderm cells. Conclusions Most embryos maintain chromosomal stability during embryonic development. Compared to cleavage stage, blastocyst stage may provide more reliable aneuploidy results.

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Section: Discussionmentioning

confidence: 99%

Chromosomal characteristics at cleavage and blastocyst stages from the same embryos

Huang

Zhao

Wang

et al. 2015

J Assist Reprod Genet

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“…Comparative genomic hybridization (CGH) and array-CGH were widely used now for PGS which could make the result more sensitive and exact [27][28][29][30].…”

Section: Discussionmentioning

confidence: 99%

Effect of maternal age on the outcomes of in vitro fertilization and embryo transfer (IVF-ET)

Yan

Tang

et al. 2012

Sci. China Life Sci.

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This is a retrospective, observational study to evaluate the effect of maternal age on the outcomes of in vitro fertilization and embryo transfer (IVF-ET). 11830 IVF-ET cycles from 10268 women were included. Four groups of different maternal age periods were compared. The groups were 2130 years old group (4549 cycles), 31-35 years old group (4424 cycles), 36-40 years old group (2429 cycles), and over 40 years old group (428 cycles). The mean starting dose of Gn and mean total dose of Gn in each cycle were significantly higher (P<0.01), while the mean retrieved oocyte number was significantly lower (P<0.01) in groups of higher maternal age period than those in each of the lower groups. The biochemical pregnancy rate and the clinical pregnancy rate were significantly lower (P<0.01), while the miscarriage rate was significantly higher (P<0.01) in groups of higher maternal age period than those in the lower groups. No difference was found in two-pronuclear zygotes (2PN) rate and good quality embryo rate among different groups. Birth defect rate was also comparable in the born babies in different groups. In the group with patients' age over 40 years old, the pregnancy rate was 26.87%, the clinical pregnancy rate was 19.39%, while the miscarriage rate after clinical pregnancy was 36.14%. To draw the conclusion, patients with higher maternal age had worse IVF outcomes. In women of fertile age, patients between 20 and 30 years old have the best IVF outcomes. Patients over 40 years old have poor IVF outcome and high miscarriage rate, which suggested the necessity of preimplantation genetic screening (PGS).in vitro fertilization, outcome, pregnancy rate, miscarriage rate, birth defect, maternal age Citation:Yan J H, Wu K L, Tang R, et al. Effect of maternal age on the outcomes of in vitro fertilization and embryo transfer (IVF-ET). Sci China Life Sci, 2012, 55: 694 -698,

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“…This limitation in FISH technology indicates a need for newer technologies like metaphase comparative genomic hybridization (mCGH), array Comparative Genomic Hybridization (aCGH), Qualitative Polymerase Chain Reaction(qPCR), Single Nucleotide Polymorphism (SNP) array and more recently NextGeneration Sequencing (NGS). These technologies are capable of analyzing all the 23 chromosome pairs from a single cell and have been shown to improve IVF clinical pregnancy rates by 32% in a randomized controlled trial [47][48][49][50][51][52][53][54].…”

Section: Time Lapse Monitoringmentioning

confidence: 99%

Selecting a Single Embryo for Transfer after In-vitro Fertilization: A Translational Medicine Perspective

Faduola¹

2015

Transl Biomed

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Validation of microarray comparative genomic hybridization for comprehensive chromosome analysis of embryos

Cited by 287 publications

References 38 publications

Chromosomal characteristics at cleavage and blastocyst stages from the same embryos

Chromosomal characteristics at cleavage and blastocyst stages from the same embryos

Effect of maternal age on the outcomes of in vitro fertilization and embryo transfer (IVF-ET)

Selecting a Single Embryo for Transfer after In-vitro Fertilization: A Translational Medicine Perspective

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