Validation of Marek's Disease Diagnosis and Monitoring of Marek's Disease Vaccines from Samples Collected in FTA^®Cards

Replication of recombinant herpesvirus of turkey expressing genes of infectious laryngotracheitis virus in specific pathogen free and broiler chickens followingin ovoand subcutaneous vaccination

“…However, in previous work it has been demonstrated that blood is not an adequate sample to detect HVT (Cortes et al, 2011). FP samples can be collected in cell lysis solution (Cortes et al, 2011), frozen (Cortes et al, 2011) or on FTA † cards (Cortes et al, 2009). Plaque assay from splenocytes or buffy coats cells could also be used for virus detection.…”

Assessment of FTA card employment for Pasteurella multocida DNA transport and detection of virulence-associated genes in strains isolated from fowl cholera in the United States

“…The transport of biological material is regulated by international and national standards, which are often complex (Brasil, 2009;Guidance…, 2009). FTA cards (Flinders Technology Associates Filter Paper -WHATMAN ® ), which are chemically treated with substances that lyse the cells and denature the proteins, are safe alternatives for the transport of inactivated microorganisms (Perozo et al, 2006;Cortes et al, 2009). This technology simplifies sample storage, transport and extraction, thereby reducing the cost and time required for diagnosis (Mbogori et al, 2006).…”

“…This technology simplifies sample storage, transport and extraction, thereby reducing the cost and time required for diagnosis (Mbogori et al, 2006). FTA cards have been employed in studies for transport and subsequent detection of poultry pathogens by PCR or RT-PCR (Moscoso et al, 2004;Perozo et al, 2006;Cortes et al, 2009;Pulido-Landínez et al, 2012;Awad et al, 2014).…”

Marek’s Disease and Differential Diagnosis with Other Tumor Viral Diseases of Poultry

“…ALV-induced lymphomas are formed by homogeneous population of lymphoblast, whereas MDV-induced lymphomas are formed by a heterogeneous population of cells, including large, medium, and small lymphocytes and macrophages. Samples can be stored frozen at À 70 1C or can be collected in Flinders Technology Associates (FTA) cards (Cortes et al, 2009). One of the major challenges in the diagnosis of MD by histopathology is the differentiation between inflammatory lesions induced by MDV and development of lymphomas also induced by MDV.…”

“…Evaluation of MD vaccines in the chickens has been traditionally done by plaque assay (Cho, 1981). Sample can be taken fresh and stored frozen until processed or in FTA cards (Cortes et al, 2009). It has been recently demonstrated that feather pulp is an adequate sample to monitor MD vaccination for vaccines of all three serotypes (Cortes et al, 2011;Baigent et al, 2005).…”