Validation of impregnation process for homeopathic globules by spectrophotometric-UV method

Abstract

We compared the impregnation techniques for globules according to the Manual of Technical Norms for Homeopathic Pharmacies (MNTFH) of the Association of Homeopathic Pharmacists (ABFH), Brazilian Homeopathic Pharmacopoeia (FHB) and variations of these techniques. The variables were evaluated in this procedure, three different sizes of globules (N o . 3, 5 and 7), the hydroalcoholic solution of 70% (v/v) Minoxidil 2% (w/v) was used to impregnate the globules in concentrations of 2, 3, 4, 5% (v/w) and the impregnation at 10 %(v/w) was used hydroalcoholic solutions at 70, 80 and 90% (v/v), and four impregnation techniques various (A-glass, B-paper, C-cup and D-FHB). As the results of content uniformity did not demonstrate a normal distribution, the one way ANOVA and a nonparametric statistical model were used for evaluation. Considering the average, the standard deviation (SD), the individual variance of each group and the principal components analysis graphs (PCA), it was observed that the "A" impregnation of globules technique, with 5% (v/w) of the impregnation concentrations and the No.5 globule presented the best uniformity of dose. As to the drying, there was a need to use a heat source.Uniterms: Homeopathic globules/impregnation process. Homeopathic compounding/evaluation. Homeopathic dose/uniformity of content.Compararam-se as técnicas de impregnação para glóbulos segundo o Manual de Normas Técnicas para Farmácias Homeopáticas (MNTFH) da Associação Brasileira de Farmacêuticos Homeopatas (ABFH), Farmacopeia Homeopática Brasileira (FHB) e variações destas técnicas. As variáveis avaliadas neste processo foram: três tamanhos diferentes de glóbulos (n.º 3, 5 e 7); a solução hidroalcóolica a 70% (v/v) de minoxidil a 2% (p/v) foi utilizada para impregnar os glóbulos nas concentrações de 2, 3, 4, 5% (v/p) e na impregnação a 10% (v/p) utilizaram-se as soluções hidroalcóolicas a 70, 80 e 90% (v/v); e quatro técnicas de impregnação diferentes (A-vidro, B-papel, C-copo e D-FHB). A impregnação foi validada através da uniformidade de dose por conteúdo, sendo o minoxidil a substância quantificada. Como os resultados da uniformidade de dose por conteúdo não demonstraram distribuição normal, utilizaram-se o One way ANOVA e um modelo estatístico não paramétrico para sua avaliação. Considerando-se a média, o desvio padrão (DP), a variância individual de cada grupo e os gráficos de análise de componentes principais (ACP), observou-se que a impregnação que utilizou o glóbulo nº5, a concentração para impregnação de 5% (v/p), graduação alcoólica de 70% (v/v) e técnica "A" apresentou a melhor uniformidade de dose. Quanto à secagem, verificou-se a necessidade do uso de uma fonte de calor.Unitermos: Glóbulos homeopáticos/processo de impregnação. Composição homeopática/avaliação. Dose homeopática/uniformidade de conteúdo.

Seaweed Calliblepharis jubata and Fucus vesiculosus Pigments: Anti-Dermatophytic Activity

“…One of these advantages is their degradability into less harmful substances for the environment [1]. Interest in seaweeds has been growing in the past few years due to their wide range of biological actions, including anticancer, anti-inflammatory, antioxidant, and anti-obesity [2][3][4].…”

“…Pigments exhibit advantageous biological activities such as antioxidants, antidiabetic, antiangiogenic, anti-inflammatory, and anticancer. These qualities make them suitable for use in these various industrial contexts [2][3][4]. As a result, they have a great chance of meeting current market needs, which increasingly focus on the health and biotechnology sectors in search of natural substances and products with established positive impacts on human health [15,16].…”

Seaweeds <em>Calliblepharis jubata</em> and <em>Fucus vesiculosus</em> Pigments: Antidermatophytic Activity

“…One of these advantages is their degradability into less harmful substances for the environment [1]. The interest in seaweed has been growing in the past few years due to the wide range of biological actions, including anticancer, anti-inflammatory, antioxidant, anti-obesity [2][3][4] .…”

“…Pigments exhibit advantageous biological activities such as antioxidants, antidiabetic, antiangiogenic, antiinflammatory and anticancer. These qualities make them suitable for use in these various industrial contexts [2][3][4] As a result, they have a great chance of meeting current market needs, which increasingly focus on the health and biotechnology sectors in search of natural substances and products with established positive impacts on human health [15,16].…”