Validation of IgG, IgM multiplex plasmonic gold platform in French clinical cohorts for the serodiagnosis and follow-up of Toxoplasma gondii infection

Pomarès, Christelle; Zhang, Bo; Arulkumar, Shylaja; Gonfrier, Géraldine; Marty, Pierre; Zhao, Su; Cheng, Steven; Tang, Meijie; Dai, Hongjie; Montoya, José G.

doi:10.1016/j.diagmicrobio.2016.09.001

Cited by 24 publications

(17 citation statements)

References 28 publications

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“…Serology for detection of IgM and IgG should be done in recognition of toxoplasmosis while IgA test gives supplementary evidence concerning reactivation or acute infection. An increase of IgM, IgG and IgA levels occurs in an acute infection while high IgG and IgA with negative tests for IgM occurs in reactivation but not in acute infection (16). These findings explain the high levels of IgG, IgM and IgA in the present study since all of the recruited patients were with acute infection of T. gondii.…”

Section: Discussionsupporting

confidence: 50%

Toxoplasmosis and Risk of Endothelial Dysfunction: Role of Oxidative Stress and Pro-Inflammatory Mediators

Al-Kuraishy

Al-Kuraishi

Al-Windy

et al. 2020

Arch Clin Infect Dis

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Background: Infection with Toxoplasma gondii (T. gondii) leads to activation of T-helper cells (Th-1 and Th-2) which are involved in the synthesis and release of different cytokines which may lead to endothelial dysfunction. Objectives: To evaluate the endothelial function in patients with acute toxoplasmosis. Methods: This case-control study involved 31 patients with toxoplasmosis aged 19 -47 years matched with 20 healthy subjects. Anti-T. gondii antibody (IgG, IgM, IgA) was determined by direct antigen-antibody reaction. Interleukin-6(IL-6), endothelin-1 (ET-1) and human malondialdehyde (MDA) serum levels were measured. Results: IgM, IgG and IgA levels were high in the infected patients compared with controls (P < 0.01). Furthermore, IL-6 serum level was high in the infected patients compared with controls (P < 0.01). In addition, ET-1 level was high in acute toxoplasmosis (7.29 ± 4.59 pg/mL) compared with controls (3.11 ± 1.69 pg/mL) (P < 0.01). In addition, MDA serum level was high (9.34 ± 4.17 nmol/mL) compared with controls (2.87 ± 1.13 nmol/mL), (P < 0.01). In acute toxoplasmosis IgM serum level was significantly correlated with IgG (r = 0.55, P = 0.001), IgA (r = 0.57, P = 0.0008), IL-6 (r = 0.45, P = 0.01), ET-1 (r = 0.51, P = 0.003) and MDA (r = 0.85, P = 0.0001). Conclusions: Acute toxoplasmosis is associated with significant oxidative stress and pro-inflammatory changes which contribute to development of endothelial dysfunction.

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Section: Discussionsupporting

confidence: 50%

Toxoplasmosis and Risk of Endothelial Dysfunction: Role of Oxidative Stress and Pro-Inflammatory Mediators

Al-Kuraishy

Al-Kuraishi

Al-Windy

et al. 2020

Arch Clin Infect Dis

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“…Additional cost savings and maternal-fetal health benefit could occur with multiplexed testing at the beginning and end of gestation. This could screen the patient not only for Toxoplasma infection, as with the Toxoplasma ICT IgG-IgM POC testing, but also for HIV, syphilis, hepatitis B, CMV IgG and IgM, herpes zoster, and immunity to rubella [24, 25]. Inclusion of other pathogens, such as Zika virus or Trypanosoma cruzi , the causative agent of Chagas disease, could also be beneficial in areas where those infections are prevalent.…”

Section: Discussionmentioning

confidence: 99%

Point-of-care testing for Toxoplasma gondii IgG/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries

et al. 2017

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BackgroundCongenital toxoplasmosis is a serious but preventable and treatable disease. Gestational screening facilitates early detection and treatment of primary acquisition. Thus, fetal infection can be promptly diagnosed and treated and outcomes can be improved.MethodsWe tested 180 sera with the Toxoplasma ICT IgG-IgM point-of-care (POC) test. Sera were from 116 chronically infected persons (48 serotype II; 14 serotype I-III; 25 serotype I-IIIa; 28 serotype Atypical, haplogroup 12; 1 not typed). These represent strains of parasites infecting mothers of congenitally infected children in the U.S. 51 seronegative samples and 13 samples from recently infected persons known to be IgG/IgM positive within the prior 2.7 months also were tested. Interpretation was confirmed by two blinded observers. A comparison of costs for POC vs. commercial laboratory testing methods was performed.ResultsWe found that this new Toxoplasma ICT IgG-IgM POC test was highly sensitive (100%) and specific (100%) for distinguishing IgG/IgM-positive from negative sera. Use of such reliable POC tests can be cost-saving and benefit patients.ConclusionsOur work demonstrates that the Toxoplasma ICT IgG-IgM test can function reliably as a point-of-care test to diagnose Toxoplasma gondii infection in the U.S. This provides an opportunity to improve maternal-fetal care by using approaches, diagnostic tools, and medicines already available. This infection has serious, lifelong consequences for infected persons and their families. From the present study, it appears a simple, low-cost POC test is now available to help prevent morbidity/disability, decrease cost, and make gestational screening feasible. It also offers new options for improved prenatal care in low- and middle-income countries.

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“…The approximate time for the detection of IgG, IgM, and IgA antibodies with a plasmonic gold chip is about 2 h at USD10 per patient [74]. With~1 μl serum sample, the multiplex plasmonic gold platform has been used for the detection of IgG/IgM in seroconverted, chronically infected, non-infected, and newborns in Nice, France [77]. Other advantages of the plasmonic biosensor are its potential to be miniaturized and multiplexed.…”

Section: Optical and Piezoelectric Biosensorsmentioning

confidence: 99%

Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals

Khan

Noordin

2019

Eur J Clin Microbiol Infect Dis

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Infection by Toxoplasma gondii is prevalent worldwide. The parasite can infect a broad spectrum of vertebrate hosts, but infection of fetuses and immunocompromised patients is of particular concern. Easy-to-perform, robust, and highly sensitive and specific methods to detect Toxoplasma infection are important for the treatment and management of patients. Rapid diagnostic methods that do not sacrifice the accuracy of the assay and give reproducible results in a short time are highly desirable. In this context, rapid diagnostic tests (RDTs), especially with point-of-care (POC) features, are promising diagnostic methods in clinical microbiology laboratories, especially in areas with minimal laboratory facilities. More advanced methods using microfluidics and sensor technology will be the future trend. In this review, we discuss serological and molecular-based rapid diagnostic tests for detecting Toxoplasma infection in humans as well as animals.

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Validation of IgG, IgM multiplex plasmonic gold platform in French clinical cohorts for the serodiagnosis and follow-up of Toxoplasma gondii infection

Cited by 24 publications

References 28 publications

Toxoplasmosis and Risk of Endothelial Dysfunction: Role of Oxidative Stress and Pro-Inflammatory Mediators

Toxoplasmosis and Risk of Endothelial Dysfunction: Role of Oxidative Stress and Pro-Inflammatory Mediators

Point-of-care testing for Toxoplasma gondii IgG/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries

Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals

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