Validation of Growth Differentiation Factor (GDF-15) as a Radiation Response Gene and Radiosensitizing Target in Mammary Adenocarcinoma Model

Hegyesi, Hargita; Lambert, James R.; Sándor, Nikolett; Schilling-Tóth, Boglárka; Sáfrány, Géza

doi:10.5772/21019

Cited by 3 publications

(6 citation statements)

References 49 publications

(51 reference statements)

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“…This study is the first to demonstrate the role of GDF15 on LM2 cell survival in vitro. The elevation of GDF15 increased cell survival, confirming our previous observations [19]. Moreover, the tumor-derived GDF15 was able to significantly increase cell survival in LM2 cells treated with conditioned medium.…”

Section: Discussionsupporting

confidence: 79%

“…After the radiation, the cells were incubated 7-9 days at 37°C. After incubation time the colonies were stained and counted for colony assay, and the survival fractions and plating efficiency were calculated [19].…”

Section: Clonogenic Assaymentioning

confidence: 99%

“…All samples were run in duplicate. Relative -fold inductions were calculated by the DDCT method, as previously used [19], with averaged relative levels of ACTB, PolG2 and HGPRT used for normalization. PolG2 Forward primer: 5'-TGTGGTCACTCAGGTCAACAAGGT-3' and Reverse:…”

Section: Quantitative Rt-pcrmentioning

confidence: 99%

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Role of GDF15 in radiosensitivity of breast cancer cells

et al. 2014

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The Growth Differentiation Factor-15 gene (GDF15) is a member of TGF-b superfamily and this cytokine family is considered to be a promising target for cancer therapy. The purpose of this study was to investigate the effect of tumor derived GDF15 on proliferation and radiosensitivity of breast cancer cells in vitro and in vivo. A mouse breast cancer LM2 cell line with stable transfection of full-length mouse GDF15 cDNA was established. Cell growth and proliferation was observed using WST assay and impedance-based method. Radiation induced GDF15 and TGF-b1 expression was determined by qRT-PCR. Radiosensitivity was measured by a colony formation assay in vitro and by a tumor growth delay assay in vivo. Cells with more than a 10-fold increase in GDF15 expression had a higher growth rate than parental control cells in vitro and in vivo. The radiation induced elevation of the expression of TGFb1 was reduced in GDF15 overexpressing cells. GDF15 may play a role in the radiation response of breast cancer cells by effecting cell survival, inhibiting radiation-induced cell death, and inhibiting the TGF-b1 related cytotoxic action.

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Section: Discussionsupporting

confidence: 79%

Section: Clonogenic Assaymentioning

confidence: 99%

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Role of GDF15 in radiosensitivity of breast cancer cells

et al. 2014

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“…Formerly, we also reported that ectopic downregulation of GDF-15 (by shGDF-15) combined with irradiation reduced the in vitro clonogenic survival of mouse mammary carcinoma cells [28]. Beside this, Chang et al reported that in nasopharyngeal cancer cells knocking down GDF-15 resulted in growth delay and in a reduction of clonogenic survival in response to radiation [29].…”

Section: Discussionmentioning

confidence: 91%

Growth Differentiation Factor-15 (GDF-15) is a potential marker of radiation response and radiation sensitivity

Sándor

Schilling-Tóth

Kis

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…As seen in our study, GDF15 also showed a dose‐dependent expression. In a microarray investigation, GDF15 was significantly induced by radiotherapy in breast cancer tissue . Moritake et al.…”

Section: Discussionmentioning

confidence: 99%