Validation of germination rate and root elongation as indicator to assess phytotoxicity with Cucumis sativus

Wang, Xiaodong; Sun, Cheng; Gao, Shixiang; Wang, Liansheng; Han, Sang‐Ik

doi:10.1016/s0045-6535(00)00520-8

Cited by 170 publications

(80 citation statements)

References 26 publications

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“…Seeds inoculated in solidified MS medium without any suspension of MNPs were taken as control. Seed germination is extensively used in phytotoxicity test because it is simple, low cost and most suitable for unstable samples or chemicals (Munzuroglu and Geckil 2002;Wang et al 2001). Maximum germination frequency was observed in seeds treated with AgNPs suspensions, while minimum seed germination was seen in seeds treated with AuNPs.…”

Section: Effect Of Mnps Suspension On Seed Germinationmentioning

confidence: 99%

In vitro seed germination and biochemical profiling of Artemisia absinthium exposed to various metallic nanoparticles

et al. 2017

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In the recent years, nanotechnology has attained much attention in the every field of science. The synthesis, characterisation and applications of metallic nanoparticles (MNPs) have become an important branch of nanotechnology. In the current study, MNPs were synthesised through polyols process and applied in vitro to study their effect on medicinally important plant : Artemisia absinthium. The current study strives to check the effect of MNPs, i.e., Ag, Cu and Au on seed germination, root and shoot length, seedling vigour index (SVI) and biochemical profiling in A. absinthium. The seeds were inoculated on MS medium supplemented with various combinations of MNPs suspension. The seed germination was greatly influenced upon the application of MNPs and was recorded highest for the silver nanoparticles (AgNPs) suspensions. The best result for seed germination (98.6%) was obtained in MS medium supplemented with AgNPs as compared to control (92.9%) and other nanoparticles, i.e., copper (69.6%) and gold (56.5%), respectively, after 35 days of inoculation. Significant results were obtained for root length, shoot length and SVI in response to application of AgNPs as compared to copper nanoparticles (CuNPs) and gold nanoparticles (AuNPs). These nanoparticles (NPs) could induce stress in plants by deploying the endogenous mechanism. In response to these stresses, plants produce various defence compounds. Total phenolic content (TPC) and total flavonoid content (TFC) were significant in the MS medium supplemented with AgNPs as compared to other NPs, while DPPH radical scavenging assay (RSA) was highest in AuNPs treated plantlets. The MNPs showed higher toxicity level and enhanced secondary metabolites production, total phenolic content, total flavonoid content, antioxidant activity, superoxide dismutase (SOD) activity and total protein content.

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Section: Effect Of Mnps Suspension On Seed Germinationmentioning

confidence: 99%

In vitro seed germination and biochemical profiling of Artemisia absinthium exposed to various metallic nanoparticles

et al. 2017

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“…Seed germination and root elongation comprise a rapid and widely used acute phytotoxicity test method possessing several advantages: sensitivity, simplicity, low cost, and suitability for unstable chemicals or samples (Munzuroglu and Geckil 2002;Wang et al 2001).…”

Section: Phytotoxicity Of the Zno Npsmentioning

confidence: 99%

Phytotoxicity of ZnO nanoparticles and the released Zn(II) ion to corn (Zea mays L.) and cucumber (Cucumis sativus L.) during germination

Zhang

et al. 2015

Environ Sci Pollut Res

120

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Toxicity of engineered nanoparticles on organisms is of concern worldwide due to their extensive use and unique properties. The impacts of ZnO nanoparticles (ZnO NPs) on seed germination and root elongation of corn (Zea mays L.) and cucumber (Cucumis sativus L.) were investigated in this study. The role of seed coats of corn in the mitigation toxicity of nanoparticles was also evaluated. ZnO NPs (1,000 mg L −1 ) reduced root length of corn and cucumber by 17 % (p<0.05) and 51 % (p<0.05), respectively, but exhibited no effects on germination. In comparison with Zn 2+

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“…Some studies show that leachate can cause deleterious effects on organisms at various trophic levels (Bakare and Osibanjo, 2000;Christensen et al, 2001;Bortolotto et al, 2009;Li et al, 2010). Among the various toxicity tests available, the seed germination and root elongation is a widely used, quick and accurate phytotoxicity test, showing sensitivity, simplicity, low cost and suitability for unstable chemicals or samples (Wang et al, 2001). Different model species are used in bioassays, including lettuce (Lactuca sativa L.), rocket plant (Eruca sativa Mill.)…”

Section: Introductionmentioning

confidence: 99%

Evaluation of phytotoxicity of municipal landfill leachate before and after biological treatment

2015

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In the present study, leachate toxicity of a municipal solid waste landfill located in the Sinos River Valley region (southern Brazil) was evaluated using plant bioassays. Leachate toxicity was assessed by analysis of seed germination and root elongation of lettuce (Lactuca sativa L.) and rocket plant (Eruca sativa Mill.) and root elongation of onions (Allium cepa L.). Bioassays were performed by exposing the seeds of L. sativa and E. sativa and the roots of A. cepa to raw leachate, treated leachate (biological treatment) and negative control (tap water). The levels of metals detected in both samples of leachate were low, and raw leachate showed high values for ammoniacal nitrogen and total Kjeldahl nitrogen. There is a reduction in the values of several physicochemical parameters, which demonstrates the efficiency of the treatment. Both L. sativa and A. cepa showed a phytotoxic response to landfill leachate, showing reduced root elongation. However, the responses of these two plant species were different. Root elongation was significantly lower in A. cepa exposed to treated leachate, when compared to negative control, but did not show any difference when compared to raw leachate. In L. sativa, seeds exposed to the raw leachate showed significant reduction in root elongation, when compared to treated leachate and negative control. Seed germination showed no difference across the treatments. The results of the study show that plant species respond differently and that municipal solid waste landfill leachate show phytotoxicity, even after biological treatment. Palavras-chave: bioensaios com plantas, poluição da água, tratamento de efluentes, efeitos tóxicos. Keywords

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Validation of germination rate and root elongation as indicator to assess phytotoxicity with Cucumis sativus

Cited by 170 publications

References 26 publications

In vitro seed germination and biochemical profiling of Artemisia absinthium exposed to various metallic nanoparticles

In vitro seed germination and biochemical profiling of Artemisia absinthium exposed to various metallic nanoparticles

Phytotoxicity of ZnO nanoparticles and the released Zn(II) ion to corn (Zea mays L.) and cucumber (Cucumis sativus L.) during germination

Evaluation of phytotoxicity of municipal landfill leachate before and after biological treatment

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