Validation of CAPS marker WR003 for the leaf rust resistance gene Lr1 and the molecular evolution of Lr1 in wheat

Liu, Xianjun; Liu, Xinchun; Sun, Hongyan; Hao, Chunyan; Wang, Xiaoxiao; Rong, Zhijiang; Feng, Zongyun

doi:10.17221/119/2021-cjgpb

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…Haplotype-based functional markers (etc., CAPS) are more reliably and easily used for applied breeding because they are derived from SNPs within the target genes or their regulatory sequences and are more likely to be related to phenotype variations (Roso et al, 2010; Ravikiran et al, 2018; Rasheed and Xia, 2019; Liu et al, 2022). In hydroponic solutions, in our previous study, Hap3 of TaPHT1;9-4B were identified by CAPS-799 functional marker and speculated to be Pi-efficient wheat cultivars by evaluating the TaPHT1;9-4B expression levels, biomasses, and P contents (Wang et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Functional marker CAPS-799 of theTaPHT1;9-4Bgene is useful for screening phosphorus-efficient wheat cultivars

Wang,

Chen,

Shi

et al. 2024

Preprint

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Context: In our previous study, TaPHT1;9-4B, one key high-affinity Pi transporter, was found to greatly contribute to Pi acquisition and transportation, and its functional marker CAPS-799 was subsequently developed to identify its Pi-efficient elite haplotype. Objective: The study aimed to screen a varieties of wheat cultivars by using the above CAPS-799, identify its Pi-efficient elite haplotype cultivars, and reveal its physiological mechanism. Methods: Successive two-year field experiments without Pi fertilizer supply, and hydroponic experiment with low Pi (10 μM) were performed. P concentrations, biomasses, grain yields, yield components, root growth parameters, and TaPHT1;9-4B transcript levels were measured. Total P accumulation and transport efficiency, and the relative growth rates were calculated. Results: Eight Pi-efficient wheat cultivars (Hap3 haplotype) were screened out by using the CAPS-799 from 80 modern major cultivars, and in successive two-year field experiments, their grain yields, spike numbers, P absorption and transport efficiencies were significantly higher than those of Non-Hap3 haplotypes (Hap1, 2 and 4) under no Pi fertilizer supply condition, and therefore, these eight cultivars belonged to Pi-efficient elite haplotype. TaPHT1;9-4B transcript levels in roots at the early stage of grain filling period in field experiment, and the relative growth rates of total root surface areas, volumes and mean root diameters of Hap3 cultivars in hydroponic experiment, were markedly higher than other haplotypes. Conclusions: CAPS-799 was a useful functional marker for screening Pi-efficient wheat cultivars, and its Pi-efficient wheat cultivars were characterized with higher TaPHT1;9-4B transcript levels and more roots. Implications: CAPS-799 will be used to screen or develop Pi-efficient wheat cultivars.

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Section: Discussionmentioning

confidence: 99%

Functional marker CAPS-799 of theTaPHT1;9-4Bgene is useful for screening phosphorus-efficient wheat cultivars

Wang,

Chen,

Shi

et al. 2024

Preprint

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“…Changes. e wheat leaf rust resistance gene Lr1 produces a 1 344-amino-acid resistance protein with a characteristic coiled-coil nucleotide-binding site leucine-rich repeat (CC-NBS-LRR) [99]. Lr35-induced adult resistance to Puccinia triticina is mediated by the TaLr35PR5 gene [100].…”

Section: Different Lr Genes and Amino Acidsmentioning

confidence: 99%

A Review on Major Rust Resistance Gene and Amino Acid Changes on Wheat (Triticum aestivum L)

Basnet

Juliana

Bhattarai

et al. 2022

Advances in Agriculture

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Wheat ranks first in the production and productivity of staple cereal crops in the world. Several diseases, including Stripe (Puccinia striiformis f. Sp. tritici), Black (Puccinia graminis f. Sp. tritici), and Brown (Puccinia recondita), have a major negative impact on wheat output, with 20 to 80% loss annually. Growing rust-resistant varieties is the most durable, cost-effective, and environmentally friendly way to combat rust pathogens. In the present review, we provide updated information on all black stem rust, yellow leaf rust, and brown leaf rust resistance genes including chromosomal position, those derived from different sources, nature of resistance type, and amino acid changes done by this gene against rust pathogen. This study summarized the 68 black stem rust, 101 leaf rust, and 108 stripe rust resistance genes from diverse cultivars of wheat and wheat primary and secondary gene pools. This review will be valuable to wheat breeders in cloning rust-resistant genes and developing leaf as well as stem rust-resistant wheat cultivars using gene pyramiding as well as frequency multiplication through introgression of the gene of interest for disease-free, sustainable grain production of wheat. The success of pyramiding genes from other sources to bread wheat depends on the nature of germplasm, the gap between flanking marker and targeted genes, the selection of genotypes in each generation, large number of gentoyes large genotype-environment interaction, etc., which is the future area of study.

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Genetic Control of Effective Seedling Leaf Rust Resistance in Aegilops biuncialis Vis. Accessions from the VIR Collection

Kolesova,

Tyryshkin

2024

Plants

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Leaf rust (caused by Puccinia triticina Erikss., Pt) is a severe foliar disease of cultivated wheat worldwide. Severe development of the disease results in significant losses in seed yield and quality. Growing immune varieties is the most rational method for Pt control in terms of effectiveness and ecological safety. However, the gene pool of cultivated wheat is very narrow for seedling Pt effective resistance genes, which hampers breeding for this trait. One of the well-known methods to broaden genetic diversity for resistance is the introgression of highly effective genes from wild relatives into the genomes of cultivated wheat. The Aegilops L. species have been proven to be perfectly suited for this purpose. No gene for Pt resistance has been transferred to wheat from Aegilops biuncialis Vis. (Lorent’s goatgrass) up to now. Previously, we selected eight accessions of the species from the VIR (N.I. Vavilov All-Russian Institute of Plant Genetic Resources) genebank that showed a perfect level of resistance to leaf rust. In this research, we studied the genetic control of resistance using hybridological, phytopathological, and molecular analyses. According to the F1–F3 hybrid evaluation results, each accession possesses one dominant gene for Pt resistance, and genes in different accessions are allelic or very tightly linked. Phytopathological test clone analysis showed that this gene is not identical to Lr9, Lr19, Lr24, Lr39, and Lr47, which are effective against Pt populations in some areas of Russia. This conclusion was partially supported by the results of the identification of DNA markers specific to these genes in bread wheat. Thus, we identified one dominant gene (temporarily symbolized as LrBi1) for effective seedling Pt resistance; it is recommended for introgression to cultivated wheat via interspecific hybridization.

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Validation of CAPS marker WR003 for the leaf rust resistance gene Lr1 and the molecular evolution of Lr1 in wheat

Cited by 3 publications

References 30 publications

Functional marker CAPS-799 of theTaPHT1;9-4Bgene is useful for screening phosphorus-efficient wheat cultivars

Functional marker CAPS-799 of theTaPHT1;9-4Bgene is useful for screening phosphorus-efficient wheat cultivars

A Review on Major Rust Resistance Gene and Amino Acid Changes on Wheat (Triticum aestivum L)

Genetic Control of Effective Seedling Leaf Rust Resistance in Aegilops biuncialis Vis. Accessions from the VIR Collection

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