Validation of Analytical Method of Marker Compounds in Extract of Pear Pomace as a Functional Health Ingredient

조은정,; 방미애,; 조승식,

doi:10.3746/jkfn.2015.44.11.1682

Cited by 1 publication

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“…Similarly, we previously reported that extraction efficiency of caffeic acid from pear pomace decreased with increasing percentage of ethanol. Besides, the extraction efficiency of chlorogenic acid from pear pomace increased with increasing amount of ethanol [22]. We concluded that phenolic extraction maybe affected by solvent combinations.…”

Section: Resultsmentioning

confidence: 99%

HPLC Analysis, Optimization of Extraction Conditions and Biological Evaluation of Corylopsis coreana Uyeki Flos

et al. 2016

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Abstract:A method for the separation and quantification of three flavonoids and one isocoumarin by reverse-phase high performance liquid chromatography (HPLC) has been developed and validated. Four constituents present in a crude ethanolic extract of the flowers of Coryloposis coreana Uyeki, were analyzed. Bergenin, quercetin, quercitrin and isosalipurposide were used as calibration standards. In the present study, an excellent linearity was obtained with an r 2 higher than 0.999. The chromatographic peaks showed good resolution. In combination with other validation data, including precision, specificity, and accuracy, this method demonstrated good reliability and sensitivity, and can be conveniently used for the quantification of bergenin, quercetin, quercitrin and isosalipurposide in the crude ethanolic extract of C. coreana Uyeki flos. Furthermore, the plant extracts were analyzed with HPLC to determine the four constituents and compositional differences in the extracts obtained under different extraction conditions. Several extracts of them which was dependent on the ethanol percentage of solvent were also analyzed for their antimicrobial and antioxidant activities. One hundred % ethanolic extract from C. coreana Uyeki flos showed the best antimicrobial activity against the methicillin-resistant Staphylococcus aureus (MRSA) strain. Eighty % ethanolic extract showed the best antioxidant activity and phenolic content. Taken of all, these results suggest that the flower of C. coreana Uyeki flos may be a useful source for the cure and/or prevention of septic arthritis, and the validated method was useful for the quality control of C. coreana Uyeki.

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Section: Resultsmentioning

confidence: 99%