Based on molecular imprinting and nanotechnology, a molecular imprinted polymer (MIP) coupled with Fe 3 O 4 À Au nanocomposite adsorbent has been successfully developed for rapid quantification of aflatoxin B1 in foodstuff. The characterization of the prepared Fe 3 O 4 À Au and Fe 3 O 4 À Au-MIP was studied by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and vibrating sample magnetometer (VSM). The limit of detection (LOD) and limit of quantification (LOQ) were estimated to be 6.12 and 18.6 μg L À 1 , respectively. The adsorption capacity was 8.975 mg g À 1 which revealed that the synthesized magnetic MIP (MMIP) presented high selective recognition property to AFB1. The selectivity adsorption experiments indicated that compared with three different compet-etive Aflatoxins, MMIP showed excellent affinity to AFB1. It was found that the adsorption capacity of MMIP for AFB1 remained essentially the same after 6 adsorption-desorption cycle which shows the acceptable repeatability and reusability of the MMIP. The proposed method was successfully applied to the estimation of AFB1 in non-alcoholic beer and barley samples with good recoveries (94.47-97.31 %). Also, the relative standard deviation (RSD %) values were lower than 3.7 %. The selectivity, stability and reproducibility of the proposed method were acceptable. The results revealed that Fe 3 O 4 À Au@MIP could be used as an efficient and reusable adsorbent for AFB1 in food samples. This study provides a simple, inexpensive, fast, accurate and environmentally-friendly technique instead of expensive and time-consuming procedures for determining AFB1 in food matrices.