Validation of an optimised protocol for quantification of microplastics in heterogenous samples: A case study using green turtle chyme

Caron, Alexandra G.M.; Thomas, Colette R.; Berry, Kathryn; Motti, Cherie A.; Ariel, Ellen; Brodie, Jon

doi:10.1016/j.mex.2018.07.009

Cited by 21 publications

(5 citation statements)

References 34 publications

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“…It is possible that this is because few studies have attempted to extract MPs from large lipid-rich biota, where saponification is more likely to occur. Studies that have investigated larger biota (e.g., tuna, turtle, dolphin, whale) have either focused on stomach contents and not the stomach tissue (Besseling et al, 2015;Lusher et al, 2015;Chagnon et al, 2018;Hernandez-Gonzalez et al, 2018) or strategically avoided digestion with KOH (Caron et al, 2018b). One of the few studies to note the occurrence of saponification reported that KOH digestion of cod stomachs produced a "Layer of black/brown slime afloat" and recommended using a combined KOH: NaClO digestion (Enders et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Solving a Sticky Situation: Microplastic Analysis of Lipid-Rich Tissue

Dawson

Motti

Kroon

2020

Front. Environ. Sci.

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Given current concerns regarding the extent of microplastic contamination in the environment, routine monitoring for microplastics in biological tissues is becoming increasingly common place. However, complex sample matrices, such as lipid-rich tissues, require multiple pre-treatment steps which may lead to increased sample processing time and costs, and a reduction in microplastic recovery rates thereby hindering monitoring efforts. Lipid-rich (fat) tissues often pose difficulties for traditional potassium hydroxide (KOH) digestion methods due to saponification. This reaction produces a suspension of glycerol and fatty acids (soaps), which may entrap microplastics inhibiting their recovery and clog filters thus reducing the efficiency of the filtration or inhibiting it altogether. In this study, the incorporation of 100% ethanol (EtOH) to existing KOH digestion methods was found to completely redissolve the viscous saponified gel formed in these reactions, with a digestion efficiency greater than 97% for all treated lipid-rich tissue samples. Recovery of spiked polyethylene and polystyrene fragments, and rayon and polyester fibers, ranged from 93% to 100%. The addition of EtOH did not induce physical or chemical degradation on these polymers. The inclusion of an ad hoc decision-making tool within the digestion workflow reduced pre-processing time for samples and allowed for solid saponified samples to be completely redissolved. This validated workflow facilitates high through-put sampling of biota, by enabling lipidrich tissues to be filtered with a high degree of efficiency thereby successfully separating microplastics from their gelatinous matrix.

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Section: Discussionmentioning

confidence: 99%

Solving a Sticky Situation: Microplastic Analysis of Lipid-Rich Tissue

Dawson

Motti

Kroon

2020

Front. Environ. Sci.

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“…In other cases, samples are mixed using a magnetic stirrer, usually as part of the digestion process, or the sample is pulverized with the aid of a mortar. (Leslie et al 2017;Caron et al 2018) The digestion stage is essential to eliminate any remaining organic matter that it may contain from the sample matrix, which could mask the precise amount of MPs. The choice of reagent used is crucial and should be optimized in each situation.…”

Section: ;mentioning

confidence: 99%

Microplastics: challenges of assessment in biological samples and their implication for in vitro and in vivo effects

Méndez Rodríguez,

Jiménez Avalos,

Fernández Macias

et al. 2023

Environ Sci Pollut Res

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Microplastics (MPs) have attracted global interest because they have been recognized as emerging pollutants that require urgent attention. MPs are plastic particles with a size between 1 micron and 5 millimeters (1 µm-5mm); those measuring less than 1 µm are known as nanoplastics (NPs). MPs are distributed in the environment in various physical forms that depend on the original morphology of the primary plastics, the degradation process, and the erosion factors to which they were subjected. Humans may be exposed to these pollutants mainly by ingestion or inhalation, which could adversely affect human health. This review focuses on exposure pathways, sources, and evidence of adverse effects reported in vitro and in vivo studies. In addition,we highlight the application of methods and techniques to assess the precise levels of exposure to MPs in biological samples to generate knowledge about their potential threat to human health.

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“…Keberadaan mikroplastik telah ditemukan di hampir semua wilayah perairan mulai dari wilayah pesisir yang dekat dengan aktivitas manusia sebagai sumber pencemaran plastik [4]- [6] hingga ke wilayah terpencil yang jauh dari aktivitas manusia [7], [8]. Mikroplastik di lingkungan perairan ini berpotensi mengancam kehidupan biota dari trofik level terendah seperti plankton [9], [10] hingga predator seperti ikan [11]- [13], penyu [14], [15] bahkan burung laut [16].…”

Section: Pendahuluanunclassified

Komposisi Mikroplastik Pada Organ Sardinella Lemuru Yang Didaratkan Di Pelabuhan Sendangbiru, Malang

Yona¹

2021

jfmr

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Mikroplastik sebagai hasil degradasi sampah plastik besar telah mencemari lingkungan perairan. Mikroplastik di perairan ini berpotensi untuk terakumulasi dalam tubuh organisme termasuk ikan. Penelitian ini bertujuan untuk menganalisis dan membandingkan mikroplastik pada insang, saluran pencernaan dan daging ikan lemuru (Sardinella lemuru) yang didaratkan di Pelabuhan Sendangbiru, Malang. Pengumpulan sampel ikan dilakukan pada Bulan Maret 2020 dengan membeli 10 ekor ikan hasil tangkapan nelayan dengan kisaran ukuran 11−15 cm. Analisis laboratorium dilakukan mulai dari mengukur panjang (cm) dan berat (g) ikan, melakukan pembedahan masing-masing organ, destruksi bahan organik dan identifikasi mikroplastik menggunakan mikroskop. Destruksi bahan organik dilakukan dengan merendam sampel organ yang sebelumnya telah ditimbang berat basahnya menggunakan larutan H 2 O 2 30 % dan Fe(II) 0.05 M. Sampel diinkubasi selama 24 jam hingga sampel beserta bahan organik hancur. Tiga jenis mikroplastik ditemukan di seluruh sampel organ ikan dengan dominansi jenis fiber (54 %), diikuti oleh fragmen (43 %) dan film (3 %). Keberadaan mikroplastik pada ikan dapat dikaitkan dengan keberadaan mikroplastik di perairan. Komposisi jenis mikroplastik berbeda untuk setiap organ. Fragmen ditemukan paling banyak pada insang, sedangkan pada saluran pencernaan dan daging didominasi oleh jenis fiber. Film ditemukan dengan jumlah yang paling sedikit pada ketiga organ. Keberadaan mikroplastik pada insang dan saluran pencernaan berkaitan dengan interaksi langsung ikan dengan lingkungan melalui pernapasan dan proses makan, sedangkan mikroplastik pada daging karena adanya proses translokasi atau perpindahan antar organ.

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Validation of an optimised protocol for quantification of microplastics in heterogenous samples: A case study using green turtle chyme

Abstract: Graphical abstract

Cited by 21 publications

References 34 publications

Solving a Sticky Situation: Microplastic Analysis of Lipid-Rich Tissue

Solving a Sticky Situation: Microplastic Analysis of Lipid-Rich Tissue

Microplastics: challenges of assessment in biological samples and their implication for in vitro and in vivo effects

Komposisi Mikroplastik Pada Organ Sardinella Lemuru Yang Didaratkan Di Pelabuhan Sendangbiru, Malang

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