Validation of an Immunochromatographic Assay for the Multiple Detection of Specific Antibodies against HIV, HBV, and HCV

Souza, Iury O.; Schaer, Robert; Lima, Fernanda Washington de Mendonça; Filho, José Tadeu Raynal Rocha; Sousa, Thiago J.; Silva, Aretha A.; Sardi, Silvia Inês; Meyer, Roberto; Portela, Ricardo Wagner

doi:10.1128/cvi.00485-12

Cited by 6 publications

(4 citation statements)

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“…Results can be visually displayed within a few minutes. Currently, several ICAs used for the detection of bacterial and viral pathogens have been reported [ 18 , 19 ]. However, the limitations of the ICA kits in HuNoVs detection were obvious, such as the specific detection of genotypes and low sensitivity [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Broad-range and effective detection of human noroviruses by colloidal gold immunochromatographic assay based on the shell domain of the major capsid protein

Lyu

et al. 2021

BMC Microbiol

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Background Human noroviruses (HuNoVs) are a major cause of nonbacterial gastroenteritis in all age groups worldwide. HuNoVs can be detected in vitro using molecular assays such as RT-PCR and RT-qPCR. However, these molecular-based techniques require special equipment, unique reagents, experienced personnel, and extended time to obtain results. Besides, the diversity of viral genotypes is high. Therefore, methods that are rapid, broad-range and effective in the detection of HuNoVs are desiderated for screening the feces or vomit of infected people during outbreaks. Results In this study, a colloidal-gold-based immunochromatographic assay (ICA) was developed for effective detection of HuNoVs in clinical samples. Monoclonal antibodies (MAbs) against the shell (S) domain in the major capsid protein of HuNoVs were used in the ICA. The limitations of detection for HuNoVs in clinical samples were 1.2 × 106 genomic copies per gram of stool sample (gc/g) and 4.4 × 105 gc/g for genogroup I and II (GI and GII) HuNoVs, respectively. A total of 122 clinical samples were tested for HuNoVs by ICA and compared against RT-qPCR. The relative sensitivity, specificity and agreement of ICA was 84.2% (95% CI: 83.6–84.8%), 100.0% (95% CI: 98.5–100.0%) and 87.7% (95% CI: 85.6–89.8%), respectively. No cross-reaction with other common enteric viruses or bacteria was observed. The ICA detected a broad range of genotypes, including GI.1, GI.3, GI.4, GI.6, GI.14, GII.2, GII.3, GII.4, GII.6, GII.13, and GII.17 HuNoVs. Conclusions This study demonstrates that ICA targeting the S domain of VP1 is a promising candidate for effectively identifying the different genotypes of HuNoVs in clinical samples with high sensitivity and specificity.

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Section: Introductionmentioning

confidence: 99%

Broad-range and effective detection of human noroviruses by colloidal gold immunochromatographic assay based on the shell domain of the major capsid protein

Lyu

et al. 2021

BMC Microbiol

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show abstract

“…Results can be visually displayed within a few minutes. Currently, several ICAs used for the detection of bacterial and viral pathogens have been reported [18,19]. However, the limitations of the ICA kits in HuNoVs detection were obvious, such as the specific detection of genotypes and low sensitivity [20].…”

Section: Introductionmentioning

confidence: 99%

Broad-range and Effective Detection of Human Noroviruses by Colloidal Gold Immunochromatographic Assay Based on the Shell Domain of the Major Capsid Protein

Lyu

et al. 2021

Preprint

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Background: Human noroviruses (HuNoVs) are a major cause of nonbacterial gastroenteritis in all age groups worldwide. HuNoVs can be detected in vitro using molecular assays such as RT-PCR and RT-qPCR. However, these molecular-based techniques require special equipment, unique reagents, experienced personnel, and extended time to obtain results. Besides, the diversity of viral genotypes is high. Therefore, methods that are rapid, broad-range and effective in the detection of HuNoVs are desiderated for screening the feces or vomit of infected people during outbreaks.Results: In this study, a colloidal-gold-based immunochromatographic assay (ICA) was developed for effective detection of HuNoVs in clinical samples. Monoclonal antibodies (MAbs) against the shell (S) domain in the major capsid protein of HuNoVs were used in the ICA. The limitations of detection for HuNoVs in clinical samples were 1.2×106 genomic copies per gram of stool sample (gc/g) and 4.4×105 gc/g for genogroup I and II (GI and GII) HuNoVs, respectively. A total of 122 clinical samples were tested for HuNoVs by ICA and compared against RT-qPCR. The relative sensitivity, specificity and agreement of ICA was 84.2 % (95% CI: 83.6-84.8 %), 100.0 % (95 % CI: 98.5-100.0 %) and 87.7 % (95% CI: 85.6-89.8 %), respectively. No cross-reaction with other common enteric viruses or bacteria was observed. The ICA detected a broad range of genotypes, including GI.1, GI.3, GI.4, GI.6, GI.14, GII.2, GII.3, GII.4, GII.6, GII.13, and GII.17 HuNoVs.Conclusions: This study demonstrates that ICA targeting the S domain of VP1 is a promising candidate for effectively identifying the different genotypes of HuNoVs in clinical samples with high sensitivity and specificity.

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“…The results can be read visually within a couple of minutes. Currently, several ICAs used for the detection of bacterial pathogens and viral pathogens had been reported [18,19]. However, many limitations of the ICA kits in HuNoVs detection were obvious, such as the detection of only speci c genotypes of HuNoVs and the low sensitivity [20].…”

Section: Introductionmentioning

confidence: 99%

Broad-range and Effective Detection of Human Noroviruses by Colloidal Gold Immunochromatographic Assay Based on the Shell Domain of the Major Capsid Protein

et al. 2020

Preprint

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Background: Human noroviruses (HuNoVs) are a major cause of nonbacterial gastroenteritis in all ages worldwide. As the replication of HuNoVs in vitro is immature, the detection of HuNoVs is depended on molecular assays such as RT-PCR and RT-qPCR. However, these molecular-based techniques require special equipment, unique reagents, experienced operators to perform, and extended time to get results. In addition, the diversity of viral genotypes is high. Therefore, a method for rapidly, broad-range, and effective detectionf of HuNoVs was desiderated for screening the excrement or vomit from infected people when outbreaks occur.Results: In this study, a colloidal-gold-based immunochromatographic assay (ICA) was developed for highly effective detection of HuNoVs in clinical samples. Monoclonal antibodies (MAbs) against the shell (S) domain in the major capsid protein of HuNoVs were used in the ICA. The limitations of detection for HuNoVs in clinical samples were 1.2×106 genomic copies per gram of stool sample (gc/g) and 4.4×105 gc/g for genogroup I and II (GI and GII) HuNoVs, respectively. A total of 122 clinical samples were tested for HuNoVs by ICA and compared against that by RT-qPCR. The relative sensitivity, specificity and agreement of the ICA was 84.2 % (95% CI: 83.6-84.8 %), 100.0 % (95 % CI: 98.5-100.0 %) and 87.7 % (95% CI: 85.6-89.8 %), respectively. No cross-reaction with other common enteric viruses or bacteria was observed. The ICA could detect a broad range of genotypes, including GI.1, GI.3, GI.4, GI.6, GI.14, GII.2, GII.3, GII.4, GII.6, GII.13, and GII.17 HuNoVs.Conclusions: Our results demonstrated that ICA targeting the S domain of VP1 is a promising candidate for effectively improve identifying different genotypes of HuNoVs in clinical samples with high sensitivity and specificity.

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Validation of an Immunochromatographic Assay for the Multiple Detection of Specific Antibodies against HIV, HBV, and HCV

Cited by 6 publications

References 0 publications

Broad-range and effective detection of human noroviruses by colloidal gold immunochromatographic assay based on the shell domain of the major capsid protein

Broad-range and effective detection of human noroviruses by colloidal gold immunochromatographic assay based on the shell domain of the major capsid protein

Broad-range and Effective Detection of Human Noroviruses by Colloidal Gold Immunochromatographic Assay Based on the Shell Domain of the Major Capsid Protein

Broad-range and Effective Detection of Human Noroviruses by Colloidal Gold Immunochromatographic Assay Based on the Shell Domain of the Major Capsid Protein

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